Entstehung von SRS-C in der durchströmten Meerschweinchenlunge durch Phospholipase A

Vogt, W.; Meyer, Uwe; Kunze, H.; Lufft, E.; Babilli, S.

doi:10.1007/978-3-662-39718-3_10

Cited by 7 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The appearance of prostaglandins in the effluents from guinea-pig lungs perfused with bee venom phospholipase A was recently reported (Vogt et al 1969). Formation of lipid-soluble spasmogenic principles with characteristics similar to those reported on in the present study occurred when guinea-pig lung tissue was incubated with bee venom phospholipase A (Fredholm and Strandberg 1969).…”

Section: Discussionsupporting

confidence: 86%

Efflux of Slow Reacting Substance (SRS) and Prostaglandins from Cat Paws Perfused with d‐Tubocurarine and n‐Octylamine

Strandberg

1971

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

STRANDBERG, K. Efflux of slou! reacting substance (SRS) and prostaglandins fromcat paws perfused with d-tubocurarine and n-octylamine. Acta physiol. scand. 1971. 82. 509-520.Release of histamine and formation of lipid-soluble smooth muscle stimulating principles occurred when cat paws were perfused with d-tubocurarine or n-octylamine. Regardless of releaser the lipid-soluble spasmogenic material was found to consist of two components, onc of which chromatographically behaved as prostaglandin En. Incubation of this principle with 15-hydroxy prostaglandin dehydrogenase in the presence of NAD' resulted in complete loss of the biological activity. The more polar component elicited slow, sustained contractions of the guinea-pig ileum; effects associated with SRS. The chromatographic behaviour and chemical properties of this principle were the same as those of compound 48/80 induced SRS. The doseresponse relations and the time courses for the appearance of histamine and SRS induced by d-tubocurarine and n-octylamine were studied. The release processes were inhibited by Nethylmaleimide, potassium cyanide, dinitrophenol and disodium ethylene diamine tetraacetate (EDTA). The results indicate that in the cat paw d-tubocurarine and n-octylamine release histamine and induce the formation of identical or very similar lipid-soluble spasmogenic principles by activating a common Ca"-dependent energy-requiring mechanism.

show abstract

Section: Discussionsupporting

confidence: 86%

Efflux of Slow Reacting Substance (SRS) and Prostaglandins from Cat Paws Perfused with d‐Tubocurarine and n‐Octylamine

Strandberg

1971

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

show abstract

“…These are present mostly esterified to the 2-position in phospholipids. In agreement with this view is the finding that venoms containing phospholipase A stimulates prostaglandin formation in a variety of tissues (Eliasson 1959, Vogt et al 1969. It is of interest here to recall the suggestion by Chakravarty ~t al.…”

Section: Erik Anggkrd and Kjell Strandbergsupporting

confidence: 62%

Efflux of Prostaglandin E, from Cat Paws Perfused with Compound 48/80

Änggård

Strandberg

1971

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

ANGGKRD, E. and K. STRANDBERG. Efflux of prostaglandin E, from cat paws perfused with compound 48/80. Acta physiol scand. 1971. 82. 333-344. Effluents from cat paws perfused with compound 48 /80 contained histamine and lipid-soluble smooth muscle stimulating principles. Fractionation of the lipid-soluble material on silicic acid resulted in a separation into two major components with different biological properties. One component was of polar character and elicited a slow, sustained contraction of the isolated guinea-pig ileum. These are effects associated with "slow reacting substance" (SRS) by definition. The other component being less polar was examined by a variety of chromatographic procedures in combination with bioassay. I n silicic acid chromatography with linear gradient elution and in reversed phase partition chromatography all biological activity followed the radioactivity due to added 3H-labelled prostaglandin Ez (PGEz). On thin layer chromatography the smooth muscle stimulating compound cochromatographed with reference PGEz. When the spasmogenic compound isolated by silicic acid chromatography was incubated with 15hydroxy prostaglandin dehydrogenase from swine lung in the presence of NAD' complete biological inactivation occurred. Determination of ester-bound Czn fatty acids in cat skin by gas chromatography revealed that arachidonate, precursor of PGE2, was the most abundant prostaglandin precursor acid. When 3H-PGEz was infused into cat paws the parent compound as well as two metabolites, 13,14-dihydro-PGEz and 15-keto-13,14-dihydro-PGEz, appeared in the effluent. I t is concluded that PGEz constitutes part of the lipid-soluble spasmogenic material appearing in connection with histamine release in the cat paw induced by compound 48 I80.

show abstract

“…The levels of these compounds are regulated by the availability of free arachidonate, which must be liberated mainly from esterified lipids [22,69,176,309,311]. The levels of these compounds are regulated by the availability of free arachidonate, which must be liberated mainly from esterified lipids [22,69,176,309,311].…”

Section: Arachidonate and Its Metabolitesmentioning

confidence: 99%