Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis

Codran, Audrey; Royer, Corinne; Jaeck, Daniel; Bastien-Valle, Michèle; Baumert, Thomas F.; Kiény, Marie Paule; Pereira, Carlos Augusto; Martín, J. P.

doi:10.1099/vir.0.81710-0

Cited by 70 publications

(56 citation statements)

References 49 publications

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“…To determine the roles of cytoskeleton in CCCM HCV transfer, we performed the coculturing experiments in the presence of the actin polymerization inhibitor cytochalasin D (44,45). The effects of cytochalasin D on actin polymerization were confirmed by immunofluorescence staining of F actin.…”

Section: Resultsmentioning

confidence: 99%

Cell-Cell Contact-Mediated Hepatitis C Virus (HCV) Transfer, Productive Infection, and Replication and Their Requirement for HCV Receptors

Liu

2013

J Virol

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bHepatitis C virus (HCV) infection is believed to begin with interactions between cell-free HCV and cell receptors that include CD81, scavenger receptor B1 (SR-B1), claudin-1 (CLDN1), and occludin (OCLN). In this study, we have demonstrated that HCV spreading from infected hepatocytes to uninfected hepatocytes leads to the transfer of HCV and the formation of infection foci and is cell density dependent. This cell-cell contact-mediated (CCCM) HCV transfer occurs readily and requires all these known HCV receptors and an intact actin cytoskeleton. With a fluorescently labeled replication-competent HCV system, the CCCM transfer process was further dissected by live-cell imaging into four steps: donor cell-target cell contact, formation of viral puncta-target cell conjugation, transfer of viral puncta, and posttransfer. Importantly, the CCCM HCV transfer leads to productive infection of target cells. Taken together, these results show that CCCM HCV transfer constitutes an important and effective route for HCV infection and dissemination. These findings will aid in the development of new and novel strategies for preventing and treating HCV infection.

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Section: Resultsmentioning

confidence: 99%

Cell-Cell Contact-Mediated Hepatitis C Virus (HCV) Transfer, Productive Infection, and Replication and Their Requirement for HCV Receptors

Liu

2013

J Virol

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“…Primary human hepatocytes (PHH) were isolated and cultured as described (21). Human hepatoma cells Huh7.5 have been described (18,22).…”

Section: Cellsmentioning

confidence: 99%

Hepatitis C Virus Infection Sensitizes Human Hepatocytes to TRAIL-Induced Apoptosis in a Caspase 9-Dependent Manner

Lin

Gorke

Rau

et al. 2008

The Journal of Immunology

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Apoptosis of infected cells represents a key host defense mechanism against viral infections. The impact of apoptosis on the elimination of hepatitis C virus (HCV)-infected cells is poorly understood. The TRAIL has been implicated in the death of liver cells in hepatitis-infected but not in normal liver cells. To determine the impact of TRAIL on apoptosis of virus-infected host cells, we studied TRAIL-induced apoptosis in a tissue culture model system for HCV infection. We demonstrated that HCV infection sensitizes primary human hepatocytes and Huh7.5 hepatoma cells to TRAIL induced apoptosis in a dose- and time-dependent manner. Mapping studies identified the HCV nonstructural proteins as key mediators of sensitization to TRAIL. Using a panel of inhibitors targeting different apoptosis pathways, we demonstrate that sensitization to TRAIL is caspase-9 dependent and mediated in part via the mitochondrial pathway. Sensitization of hepatocytes to TRAIL-induced apoptosis by HCV infection represents a novel antiviral host defense mechanism that may have important implications for the pathogenesis of HCV infection and may contribute to the elimination of virus-infected hepatocytes.

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“…HCV is enclosed by a host cell-derived membrane and belongs to the family Flaviviridae. Several reports suggest that HCV enters host cells through general endocytosis, such as by a clathrin-mediated pathway (5,6,22). However, HCV has been suggested to replicate on a detergent-resistant membrane (DRM), including some characteristic membrane structures such as lipid rafts and membranous webs (8,9,38).…”

mentioning

confidence: 99%

Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation

Okamoto¹,

Mori²,

Komoda³

et al. 2008

J Virol

100

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Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis

Cited by 70 publications

References 49 publications

Cell-Cell Contact-Mediated Hepatitis C Virus (HCV) Transfer, Productive Infection, and Replication and Their Requirement for HCV Receptors

Cell-Cell Contact-Mediated Hepatitis C Virus (HCV) Transfer, Productive Infection, and Replication and Their Requirement for HCV Receptors

Hepatitis C Virus Infection Sensitizes Human Hepatocytes to TRAIL-Induced Apoptosis in a Caspase 9-Dependent Manner

Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation

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