This chapter examines the transformative implications of artificial intelligence (AI) and machine learning (ML) on eCommerce security and operations. AI and ML technologies are transforming the eCommerce sector by streamlining supply chains, enhancing inventory management, and providing better customer experiences through personalized recommendations. Using real-time data and predictive algorithms, businesses can efficiently manage stock levels and forecast demand. In terms of security, machine learning (ML) and artificial intelligence (AI) provide effective means of detecting and mitigating fraud, protecting personal data, and ensuring secure transactions. This chapter delves into the numerous applications and benefits of artificial intelligence (AI) and machine learning (ML) in eCommerce, highlighting their critical roles in increasing efficiency, improving consumer satisfaction, and protecting operations in a fast-paced online market.