Entrevista: Marco Antonio Cianciarullo

Cianciarullo, Marco Antonio

doi:10.11606/issn.1679-9836.v82i1-4p86-88

Cited by 2 publications

(4 citation statements)

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“…It is believed that the papillomavirus is capable of maintaining the expression of structural genes, under tight control of the regulatory mechanisms of transcription and translation (30). The L1 capsid protein of HPV, regardless of the presence or absence of L2, has the property of forming structures that mimic morphologically authentic virions, VLPs, which are being used as replacements for HPV infected cell culture in vitro (8, 14, 31, 32). However, the presence of L2 seems to confer greater stability to the virus and VLP, therefore, the viral capsid indeed seems to contribute to the virus’ infectivity (33).…”

Section: Discussionmentioning

confidence: 99%

“…In the present work, we produced HPV L1/L2 VLPs (14, 15) to investigate the possibility that these particles interact with human leukocytes, in order to understand possible mechanisms involved in these interactions. We have developed a method for the isolation of these cells (17), used in suspension at 37°C, thereby creating an ex vivo system as closely as possible to the natural conditions of HPV16 infection, thus conceiving a novel experimental model.…”

Section: Discussionmentioning

confidence: 99%

“…The production of VLPs was carried out throughout the recombinant protein expression HPV16 L1 and L2 in epithelial human cells of the HEK293T lineage, cultured as previously described (14). Transfection of these cells occur in a transient way using pUF3L1h and pUF3L2h vectors, which are regulated by the human cytomegalovirus promoter containing complete sequences of genes L1 and L2 – a method already described in detail (14, 15). In this study, besides being used to produce VLPs containing both capsid proteins, HEK293T cell line was also used as a control factor on interaction assays with human peripheral blood leukocytes.…”

Section: Methodsmentioning

confidence: 99%

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Human Papillomavirus Type 16 L1/L2 VLP Experimental Internalization by Human Peripheral Blood Leukocytes

Cianciarullo

Szulczewski

Kavati

et al. 2018

Preprint

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Human papillomavirus (HPV) accounts for hundreds of thousands of new cases of cervical cancer yearly, and half of these women die of this neoplasia. This study investigates the possibility of HPV16 to infect human peripheral blood leukocytes in ex vivo assays. We have developed a leukocyte separation method from heparinized blood samples aiming cellular integrity and viability. We have expressed humanized L1 and L2 viral capsid proteins in HEK293T epithelial human cells, transiently transfecting them with vectors encoding humanized HPVL1 and L2 genes. Recombinant L1/L2 capsid proteins and structured virus-like particles interacted with human peripheral blood mononuclear cells – lymphocytes and monocytes – and were internalised through a pathway involving CD71 transferrin receptors. This was observed, at a percentile of about 54% T-CD4, 47% T-CD8, 48% B-CD20, and 23% for monocytes-CD14. The group of polymorph nuclear cells: neutrophils-eosinophils-basophils group did not internalise any VLPs. Blockage assays with biochemical inhibitors of distinct pathways, like chlorpromazine, rCTB, filipin, nystatin, liquemin, and sodium azide also evidentiated the occurrence of virus-like particles’ indiscriminate entrance via membrane receptor on mononuclear cells. This study shows that HPV16 L1/L2 VLPs can interact with the plasma membrane surface and successfully enter lymphocytes without requiring a specific receptor.IMPORTANCEHuman papillomaviruses (HPVs) belong to the Papillomaviridae family and are classified in Alpha papillomavirus, Beta papillomavirus, Gamma papillomavirus, and Mu papillomavirus genera, based on DNA sequence of the L1 gene. They are associated with the development of benignant skin warts, cell transformation and malignant tumours. L1 is its major capsid protein, and L2 is the minor HPV capsid protein. HPV type 16 is considered a high-risk Alpha papillomavirus due to the association with 50% of cervical cancers worldwide. The most frequent HPV-associated cancer type is cervical cancer, but etiological association has also been demonstrated for carcinomas of the penis, vulva, vagina, anus and oropharynx – including base of the tongue and tonsils – regions. This study indirectly investigates the possibility of HPV16 to infect other cell types in vitro, particularly human peripheral blood leukocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Human Papillomavirus Type 16 L1/L2 VLP Experimental Internalization by Human Peripheral Blood Leukocytes

Cianciarullo

Szulczewski

Kavati

et al. 2018

Preprint

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“…Também conhecido como fator "terra", os recursos naturais podem ser compreendidos como aqueles existentes na natureza e representados pelos recursos minerais, hídricos e pelas florestas, dentre outros elementos pré-existentes no meio ambiente. Vasconcellos (2006) esclarece que os recursos naturais, como qualquer outro fator de produção, são recursos escassos, portanto finitos, e por isto deve-se fazer o emprego racional de suas propriedades, a fim de atingir maiores níveis de satisfação das necessidades e desejos da sociedade. Em razão de tal escassez, considera-se de importante valia a implementação da educação financeira para o consumo sustentável de bens e serviços, produzidos a partir do emprego dos recursos naturais que, por sua natureza, são limitados.…”

Section: Educação Financeira E Sustentabilidadeunclassified

Educação financeira à luz da BNCC: concepções de docentes do ensino profissional e tecnológico

Sousa,

Lobão,

Freitas

2023

Educ. Pesqui.

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Resumo Este estudo descreve os resultados de uma investigação a respeito das características do ensino da educação financeira, em uma das unidades de ensino do Instituto Federal de Educação, Ciência e Tecnologia do Acre (Ifac). Nesse sentido, objetivou-se verificar e caracterizar, sob a perspectiva docente, se e como ocorre a prática transversal da educação financeira no currículo dos cursos técnicos integrados, observando se tais práticas correspondem ao estabelecido pela nova Base Nacional Comum Curricular (BNCC). Trata-se de uma pesquisa com abordagem qualitativa e de caráter exploratório, da qual participaram dezesseis docentes. Os dados foram coletados via aplicação de um questionário organizado com perguntas abertas e fechadas. A análise e interpretação dos dados baseou-se na metodologia de tematização. O estudo mostrou que a prática de educação financeira, à luz da nova BNCC ainda não foi implementada no lócus de estudo e que ainda não há vestígios que apontem para iniciativas de inserção transversal da temática, o que acaba por desfavorecer a formação integral dos estudantes.

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Entrevista: Marco Antonio Cianciarullo

Cited by 2 publications

References 0 publications

Human Papillomavirus Type 16 L1/L2 VLP Experimental Internalization by Human Peripheral Blood Leukocytes

Human Papillomavirus Type 16 L1/L2 VLP Experimental Internalization by Human Peripheral Blood Leukocytes

Educação financeira à luz da BNCC: concepções de docentes do ensino profissional e tecnológico

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