Entre a igualdade e a distinção

Farias, Rita de Cássia Pereira

doi:10.47749/t/unicamp.2010.477102

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on DTT and ß-mercaptoethanol, which are known as inhibitors of thiol groups, show that sulfhydryl groups do not have an important role in the active site of Aneurinibacillus sp. PDF24 esterase and suggesting that disulfide bonds are not obligatory to maintain the enzyme conformation [23]. As EDTA did not inhibit the enzyme, it can be suggested that this enzyme is not a metalloprotein [20].…”

Section: Effect Of Detergents Organic Solvents and Potential Inhibito...mentioning

confidence: 95%

Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24

KOLCU

Şal

Beldüz

et al. 2022

Sakarya University Journal of Science

View full text Add to dashboard Cite

In this study, esterase of Aneurinibacillus sp. PDF24 strain, a thermophilic bacteria, was purified to homogenity (5.25 fold purification) by column chromotography, and characterized. The molecular weight of Aneurinibacillus sp. PDF24 esterase was determined about 40 kDa. The maximum activity of the purified esterase was analyzed at 55 °C, pH 8.5. The esterase was found to be stable at 40 ºC, 50 ºC and 60 ºC for 1 hour. Km and Vmax values for p-nitrophenyl butyrate were determined as 0.120 mM and 3164.8 U/mg, respectively. In the presence of 1 mM and 5 mM metal salts of Mg2+, Li+, Ca2+, K+, no significant change occured in enzyme activity, The activity of Aneurinibacillus sp PDF24 esterase was found to be stable also in the presence of ethanol, DMSO, EDTA, DTT and ß-mercaptoethanol. The data obtained suggest that the enzyme is a serine esterase, not a metalloprotein, and that disulfide bonds are not required to maintain enzyme conformation, and therefore, depending on its features, this esterase may be a suitable candidate for industrial applications.

show abstract

Section: Effect Of Detergents Organic Solvents and Potential Inhibito...mentioning

confidence: 95%