Entrapment of Ovalbumin into Liposomes—Factors Affecting Entrapment Efficiency, Liposome Size, and Zeta Potential

Brgles, Marija; Jurašin, Darija Domazet; Sikirić, Maja Dutour; Frkanec, Ruža; Tomašić, Jelka

doi:10.1080/08982100802312762

Cited by 57 publications

(33 citation statements)

References 41 publications

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“…Liposome prepared by using different lipids acquires different surface charge. Liposome employing pohosphatidylserine, stearylamine or dioleoyltrimethylammonium propane and phosphatidylcholine get negative, positive and neutral charge respectively (Brgles et al, 2008). On the contrary, in present study liposome prepared with phosphatidylcholine possess slightly negative charge (-1.93 mV) (Fig.…”

Section: Zeta Potentialcontrasting

confidence: 52%

Formulation and in vitro evaluation of berberine containing liposome optimized by 32 full factorial designs

Sailor¹,

Seth²,

Parmar³

et al. 2015

J App Pharm Sci

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The present study demonstrates the application of 3 2 full factorial design for optimization of berberine loaded liposome for oral administration. Thin film hydration method was used to prepare liposome and optimization was done by 3 2 full factorial designs combined with desirability function. Nine formulations were prepared by using different drug : lipid and soyphosphatidylcholine : cholesterol (SPC:CHOL) ratios and evaluated for entrapment efficiency and vesicle size. The statistical validity of model was done by analysis of variance (ANOVA). Response surface graph and contour plots were used to understand the effect of variables on responses. The optimized formulation with 0.782 desirability value was prepared and evaluated for responses. The results of entrapment efficiency and vesicle size were found to be very close with the predicted values. In addition, an optimized formulation was also characterized for zeta potential, in vitro drug release and morphology. The formulation was found to be spherical shape with an average diameter of 0.823 nm and -1.93 mV zeta potential and also shows sustained release pattern. These results support the fact that 3 2 full factorial designs with desirability function could be effectively used in optimization of berberine loaded liposome.

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Section: Zeta Potentialcontrasting

confidence: 52%

Formulation and in vitro evaluation of berberine containing liposome optimized by 32 full factorial designs

Sailor¹,

Seth²,

Parmar³

et al. 2015

J App Pharm Sci

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“…A suspension is deemed to be stable when the zeta potential is 30 mV, so the GO-NLCs created in this study were relatively stable. 23 Entrapment efficiency is the most important characteristic for estimating the quality of NLCs. In general, the methods used to separate free drugs from NLCs are ultracentrifugation, a Sephadex column, and dialysis.…”

Section: Physicochemical Characterization Of Go-nlcsmentioning

confidence: 99%

Preparation, characterization, and in vivo pharmacokinetics of nanostructured lipid carriers loaded with oleanolic acid and gentiopicrin

Zhang¹,

Lv²,

Li³

et al. 2013

IJN

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Background:The purpose of this work was to develop nanostructured lipid carriers (NLCs) loaded simultaneously with oleanolic acid and gentiopicrin. Methods: An aqueous dispersion of NLCs was prepared successfully using a film-ultrasonic method, with glycerin monostearate as the solid lipid and oleic acid as the liquid lipid. Poloxamer 188 was used as the surfactant. A central composite design was used to optimize the technologic parameters. The characteristics of the NLCs were then investigated. Results: The encapsulation efficiency was 48.34% ± 2.76%, drug loading was 8.06% ± 0.42%, particle size was 111.0 ± 1.56 nm, polydispersity index was 0.287 ± 0.01, and zeta potential was −23.8 ± 0.36 mV for the optimized NLCs. The other physicochemical properties were characterized by transmission electron microscopy and differential scanning calorimetry. Drug release followed first-order kinetics and release studies confirmed that oleanolic acid and gentiopicrin fitted a sustained-release model. Compared with NLCs loaded with oleanolic acid or gentiopicrin alone, NLCs loaded with both oleanolic acid and gentiopicrin produced drug concentrations which persisted for a significantly longer time in plasma, with a linear decrement following second-order kinetics. Aspartate and alanine aminotransferase levels were significantly lower on exposure to NLCs loaded with both oleanolic acid and gentiopicrin than in negative controls. Conclusion:The results of this study confirm that oleanolic acid and gentiopicrin can be loaded simultaneously into NLCs. Compared with oleanolic acid and gentiopicrin loaded alone, sustained release and protective effects against hepatic injury were observed using NLCs loaded with both oleanolic acid and gentiopicrin.

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“…Liposomes may acquire a negative, positive or neutral charge according to the type of lipid used in the preparation (Brgles et al, 2008). Although, in the present study, liposomes were prepared with phosphatidylcholine, which is a neutral lipid, they possessed a slight negative charge at higher cholesterol ratios and a slight positive charge at low cholesterol content (Table 1).…”

Section: Zeta Potentialmentioning

confidence: 99%

Development and characterization of polymer-coated liposomes for vaginal delivery of sildenafil citrate

2017

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Vaginal administration of sildenafil citrate has shown recently to develop efficiently the uterine lining with subsequent successful embryo implantation following in vitro fertilization. The aim of the present study was to develop sildenafil-loaded liposomes coated with bioadhesive polymers for enhanced vaginal retention and improved drug permeation. Three liposomal formulae were prepared by thin-film method using different phospholipid:cholesterol ratios. The optimal liposomal formulation was coated with bioadhesive polymers (chitosan and HPMC). A marked increase in liposomal size and zeta potential was observed for all coated liposomal formulations. HPMC-coated liposomes showed the greater bioadhesion and higher entrapment efficiency than chitosan-coated formulae. The in vitro release studies showed prolonged release of sildenafil from coated liposomes as compared to uncoated liposomes and sildenafil solution. Ex vivo permeation study revealed the enhanced permeation of coated relative to uncoated liposomes. Chitosan-coated formula demonstrated highest drug permeation and was thus selected for further investigations. Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) confirmed the successful coating of the liposomes by chitosan. Histopathological in vivo testing proved the efficacy of chitosan-coated liposomes to improve blood flow to the vaginal endometrium and to increase endometrial thickness. Chitosan-coated liposomes can be considered as potential novel drug delivery system intended for the vaginal administration of sildenafil, which would prolong system's retention at the vaginal site and enhance the permeation of sildenafil to uterine blood circulation.

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Entrapment of Ovalbumin into Liposomes—Factors Affecting Entrapment Efficiency, Liposome Size, and Zeta Potential

Cited by 57 publications

References 41 publications

Formulation and in vitro evaluation of berberine containing liposome optimized by 32 full factorial designs

Formulation and in vitro evaluation of berberine containing liposome optimized by 32 full factorial designs

Preparation, characterization, and in vivo pharmacokinetics of nanostructured lipid carriers loaded with oleanolic acid and gentiopicrin

Development and characterization of polymer-coated liposomes for vaginal delivery of sildenafil citrate

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