Entrapment of lipases in hydrophobic magnetite-containing sol-gel materials: magnetic separation of heterogeneous biocatalysts1Dedicated to Prof. Herman van Bekkum on the occasion of his 65th birthday.1

Reetz, Manfred T.; Zonta, Albin; Vijayakrishnan, V.; Schimossek, Klaus

doi:10.1016/s1381-1169(98)00043-0

Cited by 123 publications

(55 citation statements)

References 73 publications

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“…Due to their special properties, magnetic nanoparticles have been used in immunology [11] and cell separation process [12]. The successful applications of magnetic nanoparticles in the immobilization of biomolecular have been reported [13,14]. Because of their good biocompatibility, strong superparamagnetic, low toxicity and easy preparation process, magnetic nanoparticles have been employed to immobilize enzyme in different matrices [15].…”

Section: Introductionmentioning

confidence: 99%

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Zhang¹,

Yang²,

Xu³

et al. 2010

Am. J. Biomed. Sci.

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A tyrosinase biosensor based on Fe 3 O 4 -chitosan nanocomposite has been developed for the amperometric detection of dopamine by the biocatalytically liberated dopaquinone at −0.25V vs. saturated calomel electrode. The obtained bio-nanoparticles, which were attached to the surface of a glassy carbon electrode (GCE), showed excellent electrochemical characteristics and at the same time acted as mediator to transfer electrons between the enzyme and the electrode. Under optimal conditions, the biosensor showed broad linear response of 2.0×10 −8 to 7.5×10 −5 mol L −1 , with the low detection limit of 6.0×10 −9 mol L −1 and the high sensitivity of 46 μA/mM for the determination of dopamine in the presence of ascorbic acid. Such tyrosinase biosensor exhibits great promise for rapid, simple and cost-effective analysis of dopamine in the samples. This immobilization approach effectively improved the stability of the electron transfer mediator and is promising for construction of biosensor and bioelectronic devices.

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Section: Introductionmentioning

confidence: 99%

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Zhang¹,

Yang²,

Xu³

et al. 2010

Am. J. Biomed. Sci.

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“…Materiais sol-gel têm sido empregados como suporte para a imobilização de enzimas tanto por encapsulação 11,12 , como por ligação covalente 13,14 .…”

Section: Introductionunclassified

Comparação do desempenho da lipase de candida rugosa imobilizada em suporte híbrido de polissiloxano-polivinilálcool empregando diferentes metodologias

Paula¹,

Moreira²,

Braga³

et al. 2008

Quím. Nova

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a. Sara Mesquita, 2270, 60511-110 Fortaleza -CE, Brasil Recebido em 16/10/06; aceito em 25/5/07; publicado na web em 9/11/07 COMPARATIVE PERFORMANCE OF Candida rugosa LIPASE IMMOBILIZED ON POLYSILOXANE POLYVINYL ALCOHOL HYBRID SUPPORT USING DIFFERENT METHODOLOGIES. The efficiency for immobilizing microbial Candida rugosa lipase on a hybrid matrix of polysiloxane polyvinyl alcohol, by adsorption, covalent coupling and encapsulation was compared. The activities of immobilized derivatives were evaluated using p-nitrophenylpalmitate (hydrolysis) and butyric acid and butanol (esterification) as substrates. Operational stability and storage tests were also performed. Among the procedures tested, the proposed matrix was efficient for immobilizing C. rugosa lipase by adsorption and covalent coupling techniques and unsuitable for encapsulation purposes. The results reveal that better catalytic properties in both aqueous and organic media were demonstrated by the covalent coupling POS-PVA immobilized lipase, including also satisfactory half-life and good storage stability.Keywords: lipase; hybrid support; adsorption. INTRODUÇÃOEnzimas na sua forma nativa (enzimas livres) têm sido usadas por séculos na indústria de alimentos e mais recentemente nas indústrias farmacêuticas e químicas 1 . Sua estrutura e modo de ação vêm sendo gradativamente elucidados por métodos experimentais bem estabelecidos, como técnicas clássicas de Raio-X e avançadas de Ressonância Nuclear Magnética (RNM). Modernas metodologias de engenharia genética têm possibilitado a produção de enzimas em larga escala ou a modificação de sua estrutura primária, alterando, portanto, algumas de suas características físico-químicas e biológicas 2 . De igual significância são as recentes técnicas de evolução direcionada, as quais têm permitido, via modificação do DNA, a preparação de enzimas especialmente dirigidas para uma determinada finalidade, isto é, enzimas que atuam em valores extremos de pH e temperatura, bem como em presença de meios não convencionais (solventes orgânicos, fase gasosa e CO 2 supercrítico) 3 .O valor de venda de enzimas para uso em indústrias de alimentos, detergentes, especialidades químicas perfizeram um total de aproximadamente US$ 700 milhões em 1992 e aumentaram para US$ 2 bilhões em 2004 4 . De acordo com as projeções, o crescimento no mercado de enzimas é de aproximadamente 4-5% ao ano, acompanhada pela redução de preço, devido ao grande núme-ro de empresas que vêm comercializando enzimas com preços mais competitivos. Como resultado, o mercado previsto para 2009 é da ordem de 2,4 bilhões 4 .De interesse particular é o aumento do uso de enzimas imobilizadas, termo empregado por Katchalski-Katzir 5 na primeira Conferência de Engenharia Enzimática realizada em Henniker, NH, EUA em 1971, para designar enzima fisicamente confinada ou localizada numa certa região do espaço, com retenção de sua atividade catalítica, podendo ser usada repetida e continuamente 6 . Nesta técnica, a enzima fica retida no interior (poros) ou na superfície de u...

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“…The entrapment process can protect enzyme activity because of the indirect contact with the confined environment, which minimizes the effects of gas bubbles, mechanical sheer, and hydrophobic solvents [18]. Entrapment immobilizations using nanoparticles are generally based on the reverse-micelle or sol-gel technique [30][31][32][33][34]. Reetz et al [32] reported the simultaneous entrapment of a lipase Amano PS (from Pseudomonas cepacia) and nanostructured magnetite (Fe 3 O 4 ) containing hydrophobic sol-gel material.…”

Section: Entrapment Immobilizationmentioning

confidence: 99%

“…Entrapment immobilizations using nanoparticles are generally based on the reverse-micelle or sol-gel technique [30][31][32][33][34]. Reetz et al [32] reported the simultaneous entrapment of a lipase Amano PS (from Pseudomonas cepacia) and nanostructured magnetite (Fe 3 O 4 ) containing hydrophobic sol-gel material. The colloidal magnetite-containing lipase was characterized by enzyme activity and was 2-3 times higher than that of free enzyme.…”

Section: Entrapment Immobilizationmentioning

confidence: 99%

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

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Abstract:The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.

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Entrapment of lipases in hydrophobic magnetite-containing sol-gel materials: magnetic separation of heterogeneous biocatalysts1Dedicated to Prof. Herman van Bekkum on the occasion of his 65th birthday.1

Cited by 123 publications

References 73 publications

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Comparação do desempenho da lipase de candida rugosa imobilizada em suporte híbrido de polissiloxano-polivinilálcool empregando diferentes metodologias

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

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