Entrapment of Enzymes and Microorganisms in Synthetic Cross-linked Polymers and their Application in Column Techniques.

Mosbach, Klaus; Mosbach, Rolf; Wentrup, Curt; Møller, Jørgen; Sjöberg, Berndt; Bunnenberg, E.; Djerassi, Carl; Records, Ruth

doi:10.3891/acta.chem.scand.20-2807

Cited by 164 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…which laid the foundation of the current field specially for biomolecule imprinting. [19] The imprinting of organic polymers by covalent approach was first reported by Wulff's group in 1972, which involves covalent complex formation between template and the surrounding polymer.…”

Section: Molecular Imprintingmentioning

confidence: 99%

See 1 more Smart Citation

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Yin

Ulbricht

2013

J. Mater. Chem. B

View full text Add to dashboard Cite

Molecularly imprinted polymers (MIP) offer in principle a robust, cost-efficient alternative to antibodies, but it is still a challenge to develop such materials for protein recognition. Here we report the molecular imprinting of a functional polymeric hydrogel layer with whole protein lysozyme as template in two-step grafting procedure by a novel initiation approach on track-etched (TE) polyethylene terephthalate (PET) and cellulose membrane surfaces.This two-step grafting strategy is based on surface functionalization with aliphatic C-Br groups which can be used as initiator for surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated copolymerization. At first, the scaffold poly(methacrylic acid) (PMAA) was obtained through SI-ATRP of poly(tert.-butyl methacrylate) (PtBMA) and subsequent hydrolysis. Thereafter, it was assembled with the template to form a stable PMAA/protein complex. In the second step, a polyacrylamide I declare that this dissertation represents my own work, except where due acknowledgement is made.

show abstract

Section: Molecular Imprintingmentioning

confidence: 99%

“…The cooperative interactions of these multimers, monomers and polymers, with template 19 are predicted to generate imprinted polymers with higher affinity and capacity for the target molecule.…”

Section: Scaffold Imprintingmentioning

confidence: 99%

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Yin

Ulbricht

2013

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…Another widely used procedure involves covalent coupling of enzymes to glass [22]. (For further reading see in particular H. Weetall in [20] [23,24] or of intact microbial cells [24], usually by polymerisation of enzyme-acrytamide mixtures, is another widely employed technique. Prior to polymerisation the enzyme is dissolved in a solution of acrylamide and cross-linking agents such as (bis)-acrylamide.…”

mentioning

confidence: 99%

Immobilised enzymes

Mosbach¹

1976

FEBS Letters

Self Cite

View full text Add to dashboard Cite

“…Indeed the phosphate solubilization system lost more than 54 % of its solubilization efficiency at the 4 th cycle of treatment, and practically cancelled at the end of the 6 th cycle (data not shown). This limitation of the biological activity is due to the existence of a favorable microenvironment inside the gel matrix and the presence of residual monomer that leads to a toxicity of microbial cell [32].…”

Section: Resultsmentioning

confidence: 99%

Biosolubilization of Mineral Insoluble phosphates by Immobilized Fungi (Aspergillus Niger) in Fluidized Bed Bioreactor

Zeroual¹,

Chadghan²,

Hakam³

et al. 2012

J Biotechnol Biomaterial

View full text Add to dashboard Cite

Phosphorus (P) is one of the most essential macronutrients required for the growth of plants and is added to soil in the form of phosphatic fertilizers. However, because of mineral re-precipitation, large amount of applied phosphate fertilizer may become unavailable to the plant. The ability of soil microorganisms to transform insoluble forms of phosphorus to an accessible form is an important path in plant growth-promoting for increasing plant yields. In this study, Aspergillus Niger, a fungal strain isolated from agricultural soil samples, was tested for its ability to solubilize different phosphated matrixes (TCP, DCP, phosphates rock). The isolated fungus exhibits high capacities to solubilize all tested phosphates. The solubilization of insoluble phosphates was associated with a drop in the pH of the culture medium. The fungal biomass was entrapped in alginate and polyacrylamide gels and was used for solubilizing mineral phosphates in fluidized bed bioreactor. The highest specific solubilization rates were obtained when A. Niger was entrapped in alginate beads. The use of the bioreactor in consecutive cycles of solubilization showed the interest of the biomass immobilization in the stability of the bioreactor. Immobilized cells in alginate continuously solubilize phosphate even after 5 cycles of solubilization without loss of activity. The phosphorus biosolubilization performances of isolated strains may open new possibilities for their biotechnology application and allow the use of this fungus in the soil fertilization.

show abstract

Entrapment of Enzymes and Microorganisms in Synthetic Cross-linked Polymers and their Application in Column Techniques.

Cited by 164 publications

References 0 publications

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Immobilised enzymes

Biosolubilization of Mineral Insoluble phosphates by Immobilized Fungi (Aspergillus Niger) in Fluidized Bed Bioreactor

Contact Info

Product

Resources

About