2016
DOI: 10.1016/j.colsurfb.2016.06.008
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Entrapment of cross-linked cellulase colloids in alginate beads for hydrolysis of cellulose

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Cited by 33 publications
(9 citation statements)
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“…Alginate beads can be prepared by dissolving alginate in water and having it react with polyvalent metal cations such as calcium so that alginate biopolymer chains are cross-linked through the bivalent calcium cations to form an interconnected open pore network (Harper et al 2014;Klock et al 1997). This net-like three dimensional structure is suitable for the entrapment of a variety of compounds such cells (plant cells, animal cells, yeast, mold, and bacteria) (Branyik et al 2005;Verbelen et al 2006), enzymes (Nguyen et al 2016;Won et al 2005), or even synthetic chemicals.…”
Section: Introductionmentioning
confidence: 99%
“…Alginate beads can be prepared by dissolving alginate in water and having it react with polyvalent metal cations such as calcium so that alginate biopolymer chains are cross-linked through the bivalent calcium cations to form an interconnected open pore network (Harper et al 2014;Klock et al 1997). This net-like three dimensional structure is suitable for the entrapment of a variety of compounds such cells (plant cells, animal cells, yeast, mold, and bacteria) (Branyik et al 2005;Verbelen et al 2006), enzymes (Nguyen et al 2016;Won et al 2005), or even synthetic chemicals.…”
Section: Introductionmentioning
confidence: 99%
“…However, the stability of dextranase after entrapment was significantly improved compared to that of the soluble thermozyme. It has been previously computed that the thermal stability of the entrapped biocatalyst is enhanced due to the stabilization in 3D structure after entrapment [28]. The soluble enzyme reduced more than 30% of its original activity at temperature above 50°C while entrapped dextranase retained 65% residual activity at 80°C even after 2.0 hours (Figures 4(a) and 4(b)).…”
Section: Stability Of Soluble and Entrapped Dextranasementioning
confidence: 87%
“…However, it has been used in many instances to design biosensors (Cosnier ; Gupta and Chaudhury ). Moreover, enzyme leakage is a problem of this immobilization strategy, which has been usually solved by increasing the size of the enzyme molecule (attaching the enzyme to a polymer, or making an enzyme aggregate) (Wilson and others ; Matto and Husain, ; Cui and others ; Nguyen and others ). Covalent immobilization may produce a high rigidification if an intense multipoint covalent attachment is achieved, but after enzyme inactivation both enzyme and support will be discarded and the immobilization protocols are then more sophisticated (Mateo and others , Garcia‐Galan and others ).…”
Section: Improving Proteases For Biotechnology Applicationsmentioning
confidence: 99%