Entrapment of air microbubbles by ice crystals during freezing exacerbates freeze-induced denaturation of proteins

Dao, Huy Minh; Sahakijpijarn, Sawittree; Chrostowski, Robert; Peng, Han-Hsuan; Moon, Chaeho; Xu, Haiyue; Mangolini, Filippo; Huy, Hoang; Cui, Zhengrong; Williams, Robert O.

doi:10.1016/j.ijpharm.2022.122306

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…In addition, our analysis of change rates found that most samples had low change rates, but each type of allergen had 1–2 samples with change rates exceeding 30%, without close correlation to storage duration or positive level. We considered that this might be due to (1) the phenomenon of "cold denaturation", where when serum samples are stored for a long time at temperatures far below the freezing point of water, the non-polar groups inside the antibody protein are exposed to water, causing changes in the intermolecular interactions of free energy and reducing the hydrophobic interaction of proteins and changes in tertiary structure 35 , 36 ; (2) long-term freezing may lead to crystal formation of the buffering component in serum, resulting in changes in pH 37 ; (3) different reagent batches used for the previous measurements may cause errors. (4) During the thawing process, ice recrystallization can affect the structural integrity of nearby proteins, leading to protein denaturation 38 ; (5).…”

Section: Discussionmentioning

confidence: 99%

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Chang,

Hu,

Pan

et al. 2023

Sci Rep

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Research has shown that the concentration and composition of biological samples may change after long-term ultra-low temperature storage. Consequently, this study examined the effect of ultra-low temperature storage on serum sIgE detection by comparing sIgE concentrations at various durations from the time of sample storage to subsequent testing. We selected 40 serum samples from the Guangzhou Medical University Affiliated First Hospital Biobank, which had been tested for house dust mites, dog hair, tobacco mold, cockroaches, and cow milk allergen sIgE. Samples were categorized by storage duration: 14 samples stored for 10 years, 12 for 5 years, and 14 for 3 years. They were also classified by sIgE positive levels: 15 samples at levels 1–2, 15 at levels 3–4, and 10 at levels 5–6. The allergen sIgE of these samples was retested using the same technology. Regardless of the type of allergen or the level of positivity, the majority of sIgE concentrations measured at the time of storage were higher than the current measurements, but the difference was not statistically significant. The correlation between the sIgE results at the time of storage and the current results was high for samples stored for 10 years (rs = 0.991, P < 0.001) and 5 years (rs = 0.964, P < 0.001). Serum allergen sIgE is stable when stored under ultra-low temperature conditions, making the construction of a biological sample bank for allergic diseases feasible. This will facilitate researchers in quickly obtaining samples, conducting technical research, and translating findings, thereby promoting the development of the field of allergy through integration of industry, academia, and research.

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Section: Discussionmentioning

confidence: 99%

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Chang,

Hu,

Pan

et al. 2023

Sci Rep

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“…This indicates that the aggregation state of NaCl/CA-2 was changed during the freeze-drying process, and this phenomenon was somewhat similar to the protein freeze-drying process. Research shows 19 proteins undergo denaturation during freeze-drying, possibly due to the amino groups in proteins exposing to a waterdeficient environment during the sublimation of the solid ice formed on the surface of proteins in the freezing process. This changes the aggregation state of the final protein and causes its degeneration.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…In addition, our group has obtained a composite precursor of glutaraldehyde and dicyandiamide through hydrogen-bonding interactions, and this precursor structure obtains a catalyst of closer structure between carbon and carbon nitride; it is also shown that the structure of the precursor will influence the structure of the final catalyst . Interestingly, we have found that freeze-drying can also alter the structure of precursors in existing studies; for example, freeze-drying can induce protein denaturation and cause the protein to undergo a degree of aggregation . Therefore, we plan to use a freeze-drying method to treat the precursor and investigate whether it will affect the structure of the final catalyst and its impact on catalytic performance.…”

Section: Introductionmentioning

confidence: 99%

“…18 Interestingly, we have found that freezedrying can also alter the structure of precursors in existing studies; for example, freeze-drying can induce protein denaturation and cause the protein to undergo a degree of aggregation. 19 Therefore, we plan to use a freeze-drying method to treat the precursor and investigate whether it will affect the structure of the final catalyst and its impact on catalytic performance.…”

Section: ■ Introductionmentioning

confidence: 99%

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Preparation and Photocatalytic Degradation Performance of Amine-Rich Carbon Nitride with Structural Modulation of the Precursor

Shi,

Yu,

et al. 2023

Langmuir

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The photocatalytic performance of graphitic phase carbon nitride (g-C3N4) is strongly influenced by its own microstructure as well as the precursor structure that causes the microstructure changes. In this paper, a composite precursor of sodium chloride and cyanamide (NaCl/CA-2) was obtained by freeze-drying, which possess an aggregated state different from that of the non-freeze-drying method. This new aggregation state with the introduction of sodium ions into the cyanamide lattice results in a higher activation energy of NaCl/CA-2 in the thermal polycondensation process of the molten salt-assisted preparation of g-C3N4, which prevented the condensation of two cyanamides to one dicyandiamide, ultimately obtaining FD-CN with an amino-rich structure. The nitrogen atoms on the amino group can provide the photocatalyst with more unpaired electrons that can participate in the photoexcitation process, further improving its electron–hole separation ability and charge transfer efficiency, thus effectively enhancing its photocatalytic activity. Compared to the original g-C3N4, the photocatalytic activity of FD-CN for the degradation of methylene blue increased 2.19 times.

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“…Contact angle goniometry is one of the experimental methods available for quantitatively evaluating the surface energy of solids . This has made it a vital tool for the development of a number of technologies across several science and engineering fields (e.g., materials science and engineering, environmental science, medicine, petroleum engineering), such as superhydrophobic coatings for aircraft wings and processing methods for vaccines . The process of performing a sessile drop contact angle measurement has greatly been simplified by the advent of commercially available, user-friendly, and multimodal instruments, which, for example, can automatically deposit arrays of liquid droplets onto a substrate, image the drops with a high-speed camera, and simultaneously analyze the images in a matter of seconds.…”

Section: Introductionmentioning

confidence: 99%

Effect of Camera Parallax Angle on the Accuracy of Static Contact Angle Measurements

Johnson,

Mangolini

2024

Langmuir

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Measuring the contact angle at the solid/liquid/ vapor triple point in sessile drop experiments is one of the most popular and simple ways to quantify the wettability of surfaces and determine the surface free energy. Despite decades of technical advancements in contact angle measurements, which allowed for improving the precision of sessile drop measurements below ±1°, an often overlooked source of experimental error in these measurements originates from the camera's parallax angle (PA) − the angle between the camera optical axis and the sample stage surface. Here, we quantified the systematic errors in the measurement of contact angles due to the acquisition of drop images at finite PA values by simulating sessile drop experiments in which synthetic drops were created using the Young−Laplace equation. The absolute contact angle error induced by imaging drops at nonzero PAs was found to increase as the true contact angle (TCA) deviates from 90°and resulted in an overestimation (underestimation) of the contact angle for drops having TCAs lower (higher) than 90°. The computed absolute contact angle error reaches values as high as −20°(+12.2°) for drops having a TCA of 175°(5°) when imaged with a PA of 10°, thus indicating the importance of considering the PA when accurately quantifying contact angles in sessile drop experiments. The shape and, by extension, volume of the sessile drop was also found to affect the magnitude of the absolute contact angle error as sessile drops with higher apex curvatures exhibited lower absolute error than those with lower curvatures at any given PA. The outcomes of this work provide guidelines for minimizing systematic errors in sessile drop measurements due to the collection of drop images at nonzero PAs.

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Entrapment of air microbubbles by ice crystals during freezing exacerbates freeze-induced denaturation of proteins

Cited by 10 publications

References 31 publications

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Preparation and Photocatalytic Degradation Performance of Amine-Rich Carbon Nitride with Structural Modulation of the Precursor

Effect of Camera Parallax Angle on the Accuracy of Static Contact Angle Measurements

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