Entrap and release of Phe–Phe nanotubes in sol–gel derived silicate matrix: study through nanosilver interaction

Abstract

After the discovery of the ability of dipeptide self-assembly, numerous research activities have been reported on Phe-Phe dipeptide based nano-materials. However, entrapping peptide nanotubes in a silicate matrix has not been carefully studied. This paper reports on selfassembled Phe-Phe nanotubes entrapped in a silicate matrix derived by a simple, and modified acid mediated sol-gel approach. Here, interaction of silver nanoparticles with regained nanotubes from the silicate matrix is reported. Mass spectrometry data showed the self-assembly of peptide monomers. Scanning electron microscopy and transmission electron microscopy studies have been used to examine the shapes and sizes of the nano-structures, and the results provide a promising method for trapping peptide based nanotubes. UV-Visible spectra suggest that there are some effects of silicate environment around Phe-Phe NTs on its absorbance. Density functional calculations showed the probable type of interaction between peptide and Ag atoms. This study can be useful for future applications, which are based on immobilization strategy for drug carrying and sensing.

Silicate Matrix with Implanted Peptide Self-Assemblies for Smart Sensor Electrodes and Coated Glass Systems

“…The detailed synthetic procedure was adopted from the reported methods [1,4,5] and the same is shown as in Scheme 1.…”

“…Sol-gel technology is the well-known process for the production of nano structured materials and hence for several industrial and biological applications [1][2][3][4][5][6]. The capability of the self-assembly processes is an efficient methodology on finding out the simplest biomaterials like silicate entrapped biomolecules [2].…”

“…The capability of the self-assembly processes is an efficient methodology on finding out the simplest biomaterials like silicate entrapped biomolecules [2]. One example is the recognition of Alzheimer's sickness and its treatment option by bionano materials [4,5]. Phe-Phe nanotubes (NTs) show several special properties like consistent homogeneity, and stability at high temperatures [6][7][8].…”

“…Silicate matrix serve as reservoir for water, and it increases the ability to maintain the biological activity of enzymes, anti-bodies, and body fluid cells etc., [35][36][37][38][39]. These porous silicate matrices obtained by the low-temperature sol-gel method act as promising host matrix for the encapsulation of biomolecules [1,5]. So as a result, pore-size motif is one of the key elements which enables to accomplish the entrapment of wide range of bio-molecules and opens up different applications [40][41][42][43][44][45][46][47][48].…”

Simple size-selective separation and microscopic characterization of Phe–Phe nanotubes and study of its internalization inside CHO cells

“…The obtained NTs were kept for two weeks to see any changes in sizes and shapes of nanostructures. Aging caused slight increase in the length of the tubes [19]. Fluorescein isothiocyanate (FITC) dye was added to this NTs solution as 1:10 molar ratio and kept for 24 h for the tagging.…”