Entrance and survival of Salmonella typhimurium and Yersinia enterocolitica within human B- and T-cell lines

Verjans, Georges M. G. M.; Ringrose, Jeffrey H.; Alphen, L. Van; Feltkamp, T. E. W.; Kusters, Johannes G.

doi:10.1128/iai.62.6.2229-2235.1994

Cited by 36 publications

(15 citation statements)

References 41 publications

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“…Salmonella typhimurium proliferates inside several cell types, such as macrophages and epithelial cells, but not in lymphoid cells. 1,2 Spondyloarthropathies, including ReA, are strongly associated with the class I allotype human leucocyte antigen (HLA)-B27. 3 The pathogenetic role of this molecule remains unknown, but several hypotheses have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Infection with Salmonella typhimurium has no effect on the composition and cleavage specificity of the 20S proteasome in human lymphoid cells

et al. 2007

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Section: Introductionmentioning

confidence: 99%

Infection with Salmonella typhimurium has no effect on the composition and cleavage specificity of the 20S proteasome in human lymphoid cells

et al. 2007

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“…One of the mechanisms to alter regulation of immune and inflammatory responses, as demonstrated in murine macrophages, is to induce apoptosis by suppressing the cellular activation of NF‐kB, which in turn inhibits production of TNFα[11]. For human B and T cells, adherence of Yersinia , but not invasion, was found in significant amounts as well [12] .…”

Section: Introductionmentioning

confidence: 99%

Dendritic cell function is perturbed byYersinia enterocoliticainfectionin vitro

Schoppet

Bubert

Huppertz

2000

Clinical and Experimental Immunology

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SUMMARYInfection with Yersinia enterocolitica is the cause of intestinal or extraintestinal diseases. We investigated the role of dendritic cells (DC), the most potent antigen-presenting cell (APC), in the course of infection with Y. enterocolitica in vitro. For these studies, DC were isolated from human peripheral blood and infected with green fluorescent protein (GFP)-labelled Y. enterocolitica. Bacteria were found within DC by FACS analysis and viable bacteria could be cultured from lysed cells. Within 24 h after infection, DC upregulated CD83 and CD86 followed at day 3, indicating maturation of DC. In contrast, for MHC class II, a marked but transient downregulation was observed at day 3 after infection, and downregulation to a lesser extent for CD80 at day 5. To assess the immunostimulatory capacity of DC, viable infected and uninfected DC were incubated with autologous T cells in the presence of phytohemagglutinin A (PHA). T cell proliferation was significantly reduced at days 4±6 after infection but not thereafter, whereas nonpathogenic Escherichia coli was not able to mimick this suppressive effect of Y. enterocolitica. The same suppression could be observed when infected DC were used in a mixed leucocyte reaction with allogeneic T cells. Thus Y. enterocolitica is able to invade DC, does not induce necrosis or apoptosis, but affects maturation of DC. However, MHC class II-molecules are downregulated initially, which coincides with a diminished immunostimulatory capacity of DC infected with Y. enterocolitica. The diminished immunostimulatory capacity of DC following infection with Y. enterocolitica in vitro might impair or delay elimination of bacteria thereby contributing to pathogenesis of bacterial enteritis or extraintestinal manifestations such as reactive arthritis.

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“…It is well established that B cells are targets of Salmonella infection. [22][23][24][25][26][27][28] In murine models, we observed that Salmonella…”

Section: Spi-5 Encodes a Series Of Proteins Responsible For Enteropatmentioning

confidence: 99%

“…22 In vitro assays have shown that human B cell lines JY and JP and mouse B cell line A20 are able to internalize bacteria. 24,26 Additionally, it has been reported that Salmonella typhi is able to infect the immortalized B lymphoblastoid cell line (LCL). The infection is macropinocytosis-dependent because the LCL treated with amiloride (a macropinocytosis inhibitor)…”

Section: Spi-5 Encodes a Series Of Proteins Responsible For Enteropatmentioning

confidence: 99%

Beyond the antibody: B cells as a target for bacterial infection

García-Gil

Lopez-Bailon

Ortiz-Navarrete

2019

Journal of Leukocyte Biology

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It is well established that B cells play an important role during infections beyond antibody production. B cells produce cytokines and are APCs for T cells. Recently, it has become clear that several pathogenic bacterial genera, such as Salmonella, Brucella, Mycobacterium, Listeria, Francisella, Moraxella, and Helicobacter, have evolved mechanisms such as micropinocytosis induction, inflammasome down‐regulation, inhibitory molecule expression, apoptosis induction, and anti‐inflammatory cytokine secretion to manipulate B cell functions influencing immune responses. In this review, we summarize our current understanding of B cells as targets of bacterial infection and the mechanisms by which B cells become a niche for bacterial survival and replication away from extracellular immune responses such as complement and antibodies.

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Entrance and survival of Salmonella typhimurium and Yersinia enterocolitica within human B- and T-cell lines

Cited by 36 publications

References 41 publications

Infection with Salmonella typhimurium has no effect on the composition and cleavage specificity of the 20S proteasome in human lymphoid cells

Infection with Salmonella typhimurium has no effect on the composition and cleavage specificity of the 20S proteasome in human lymphoid cells

Dendritic cell function is perturbed byYersinia enterocoliticainfectionin vitro

Beyond the antibody: B cells as a target for bacterial infection

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