Entrainment of Cellular Circadian Rhythms in Lactuca sativa L. Leaf by Spatially Controlled Illuminations

Seki, Naoki; Ukai, Kazuyo; Higashi, Takanobu; Fukuda, Hirokazu

doi:10.4172/2155-6210.1000186

Cited by 5 publications

(3 citation statements)

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“…Although individual amplitudes have a direct influence on the network amplitude, the level of the cellular synchrony also determines the amplitude of the network dynamics. For instance, a gradual decrease in the oscillation amplitude of plant circadian systems under constant conditions is known to be partially due to desynchronized oscillations of the individual circadian cells (14)(15)(16)(17)(18)(19)(20). Therefore, our study focused on network synchrony as the key parameter controlling the network amplitude.…”

Section: Resultsmentioning

confidence: 99%

Multicellularity enriches the entrainment of Arabidopsis circadian clock

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Multicellularity enriches the entrainment of Arabidopsis circadian clock

et al. 2017

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“…Previous studies have reported that the plant circadian clock in leaves is also spontaneously desynchronized (Wenden et al, 2012; Muranaka and Oyama, 2016). Other studies have demonstrated that desynchronization among cells can be caused by artificial light control (Fukuda et al, 2013; Seki et al, 2015). Hence, this method of measuring PRCs can be applied to other plant organs, such as the leaf or stem, by artificially controlling the environment.…”

Section: Discussionmentioning

confidence: 99%

Unstable Phase Response Curves Shown by Spatiotemporal Patterns in the Plant Root Circadian Clock

Masuda

Fukuda

2021

J Biol Rhythms

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Phase response curves (PRCs) play important roles in the entrainment of periodic environmental cycles. Measuring the PRC is necessary to elucidate the relationship between environmental cues and the circadian clock. Conversely, the PRCs of plant circadian clocks are unstable due to multiple factors such as biotic/abiotic noise, individual differences, changes in amplitude, growth stage, and organ/tissue specificity. However, evaluating the effect of each factor is important because PRCs are commonly obtained by determining the response of many individuals, which include different amplitude states and organs. The plant root circadian clock spontaneously generates a spatiotemporal pattern called a stripe pattern, whereby all phases of the circadian rhythm exist within an individual root. Therefore, stimulating a plant root expressing this pattern enables phase responses at all phases to be measured using an individual root. In this study, we measured PRCs for thermal stimuli using this spatiotemporal pattern method and found that the PRC changed asymmetrically with positive and negative temperature stimuli. Individual differences were observed for weak but not for strong temperature stimuli. The root PRC changed depending on the amplitude of the circadian rhythm. The PRC in the young root near the hypocotyl was more sensitive than those in older roots or near the tip. Simulation with a phase oscillator model revealed the effect of measurement and internal noises on the PRC. These results indicate that instability in the entrainment of the plant circadian clock involves multiple factors, each having different characteristics. These results may help us understand how plant circadian clocks adapt to unstable environments and how plant circadian clocks with different characteristics, such as organ, age, and amplitude, are integrated within individuals.

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“…[41][42][43] Furthermore, rGO is a preferable matrix relative to GO for enzyme immobilization because of its higher enzyme loading, stability, and more desirable interactions with enzymes. [44,45] Although rGO films can prepare from GO by reducing agents, [46][47][48] the electrochemical reduction is a more favourable method to produce rGO from GO as it is more environmentally friendly, faster, and easily controllable than other methods. [49,50] Thus, this work aims to develop a sensitive biosensor by immobilization of HRP and rGO on the surface of glassy carbon electrode (GCE) for cyanide detection.…”

Section: Introductionmentioning

confidence: 99%

A sensitive biosensor for cyanide detection using modified glassy carbon electrode with reduced graphene oxide and immobilization of horseradish peroxidase

Ghanavati,

Keshavarz Moraveji,

Iranshahi

2023

Can J Chem Eng

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Cyanide is a highly poisonous and hazardous substance which may release into the environment from natural sources or industrial effluent; therefore, cyanide detection is a fundamental step to prevent environmental pollution and secure health and safety. In this study, we prepared a sensitive amperometric inhibition biosensor for cyanide detection by immobilization of horseradish peroxidase (HRP) enzyme and reduced graphene oxide (rGO) on the surface of glassy carbon electrode (GCE). To do so, we performed the amperometric measurement by modified GCE to test its efficiency in detecting cyanide. The optimum conditions of pH equal to 7.5, −100 mV applied potential, 0.7 μM mediator concentration, and 0.5 mM substrate concentration were found. Then, experiments were performed at different boundary conditions in a range of 0.1 to 10 μM cyanide concentration at optimal conditions and a low detection limit of 0.01 μM was obtained. Also, the possible mechanism of inhibition was analyzed based on the Michalis–Menten equation and non‐competitive inhibition was observed. Due to high sensitivity, low detection limit, and low cost, this biosensor is proposed as a useful method for cyanide determination in real samples.

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Entrainment of Cellular Circadian Rhythms in Lactuca sativa L. Leaf by Spatially Controlled Illuminations

Cited by 5 publications

References 0 publications

Multicellularity enriches the entrainment of Arabidopsis circadian clock

Multicellularity enriches the entrainment of Arabidopsis circadian clock

Unstable Phase Response Curves Shown by Spatiotemporal Patterns in the Plant Root Circadian Clock

A sensitive biosensor for cyanide detection using modified glassy carbon electrode with reduced graphene oxide and immobilization of horseradish peroxidase

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