Entomotoxic effects of fungal lectin from Rhizoctonia solani towards Spodoptera littoralis

Hamshou, Mohamad; Damme, Els J.M. Van; Smagghe, Guy

doi:10.1016/j.mycres.2009.10.002

Cited by 41 publications

(26 citation statements)

References 33 publications

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“…Homodimer 4 Gal na Entomotoxic (Hamshou et al 2013;Hamshou et al 2010b;Hamshou et al 2012;Skamnaki et al 2013) Bleuler-Martinez et al 2011;Fujihashi et al 2003;Fukumori et al 1990;Kochibe and Furukawa 1980;Olausson et al 2010;Olausson et al 2008;Tateno et al 2009;Wimmerova et al 2003) GlcNAc-β1,4-X na nd (Cioci et al 2006;Ueda et al 1999a;Ueda et al 2002;Ueda et al 1999b;Ueda et al 2003) Entomotoxic (Birck et al 2004;Bleuler-Martinez et al 2011;Damian et al 2005;Francis et al 2003;Jaber et al 2008;Jaber et al 2007;…”

Section: β-Trefoilmentioning

confidence: 99%

“…Strong entomotoxicity affecting development and survival of the economically important plant pests cotton leafworms Spodoptera littoralis and S. litura was shown for β-trefoil-type lectin RSA and actinoporin-type lectin SRL, respectively, both binding Gal/GalNAc-containing glycans (Hamshou et al 2010b;Vishwanathreddy et al 2014). Additionally, RSA exhibited toxicity toward the red flour beetle T. castaneum, an important pest of stored products (Walski et al 2014).…”

Section: Potential Applicationsmentioning

confidence: 99%

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Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Sabotič

Ohm

Künzler

2015

Appl Microbiol Biotechnol

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Fruiting bodies or sporocarps of dikaryotic (ascomycetous and basidiomycetous) fungi, commonly referred to as mushrooms, are often rich in entomotoxic and nematotoxic proteins that include lectins and protease inhibitors. These protein toxins are thought to act as effectors of an innate defense system of mushrooms against animal predators including fungivorous insects and nematodes. In this review, we summarize current knowledge about the structures, target molecules, and regulation of the biosynthesis of the best characterized representatives of these fungal defense proteins, including galectins, beta-trefoil-type lectins, actinoporin-type lectins, beta-propeller-type lectins and beta-trefoil-type chimerolectins, as well as mycospin and mycocypin families of protease inhibitors. We also present an overview of the phylogenetic distribution of these proteins among a selection of fungal genomes and draw some conclusions about their evolution and physiological function. Finally, we present an outlook for future research directions in this field and their potential applications in medicine and crop protection.

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Section: β-Trefoilmentioning

confidence: 99%

Section: Potential Applicationsmentioning

confidence: 99%

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Sabotič

Ohm

Künzler

2015

Appl Microbiol Biotechnol

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“…Their agglutination ability was inhibited by various carbohydrates, the best inhibition being observed when galactose or raffinose was used. The significance of the lectins in fungal pathogens is still unclear, being purposed the idea of a possible role in mycoparasitism or as a storage protein (6). In our experiments was examined how the presence in culture media of the specific carbohydrates (that inhibited in vitro the agglutinating lectins activity) influenced the interactions with antagonistic strains (Trichoderma spp.TV1, Trichoderma spp.P8 and Trichoderma spp.S37).…”

Section: Antagonistic Interactions Between Trichoderma Spp Isolates mentioning

confidence: 99%

Antifungal Action of NewTrichoderma Spp.Romanian Isolates on Different Plant Pathogens

Cornea

A²,

Matei

et al. 2009

Biotechnology & Biotechnological Equipment

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The genus Trichoderma comprises various fungal strains that can act as biological control agents against a large diversity of plant pathogens. A number of commercial products are available but the diversity of plant pathogens, and their increased resistance to the current control products (chemical or biological products) determined the search of new strains, potentially useful for biological control. New strains of Trichoderma spp. were isolated from Romanian soils and their antagonistic activity against Phytium spp and Rhizoctonia spp. was examined. The in vitro biocontrol activity of Trichoderma spp., as well as of other antagonistic fungi (Penicillium chrysogenum Gliocladium roseum and Eppicoccum purpurescens) on the plant pathogens was increased in the presence of FeCl 3 . The molecular analysis realized by ITS-RFLP and PCR with specific primers allow the confirmation of previous taxonomic determination of T.harzianum and T.viride. However, an increased intraspecific molecular polymorphism was observed using several arbitrary primers (RAPD analysis). The interactions between fungal strains (plant pathogens and antagonistic strains) were also examined, in order to determine the mechanism of action of the antifungal strains. It was observed that all of the Trichoderma strains were able to produce large amount of hydrolytic enzymes (chitinase, cellulases and proteases) and to act as mycoparasites for pathogens. The involvement of fungal lectins in the interactions was also examined. Fungal extracts obtained from the best antagonists were tested for the induction of plant (soybean) resistance against pathogens. Higher levels of plant enzymes PAL, POX and chitinase were observed, and correlated with an increased resistance to artificial infection. The results obtained could be used in further experiments to establish new approaches of "plant immunization" with microbial products.

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“…In animals, intracellular lectins are involved in protein quality control (Lederkremer 2009), while cell surface lectins play a role in adhesion, cell-to-cell interactions, and innate immunity (Fujita 2002). Plant lectins, which are mostly extracellular, are believed to play a role in interactions with other organisms, either in defence or in recognition, and Jacalin-related lectins have been described to play a role as storage proteins and/or in defence ( van Damme et al 2004; (Hamshou et al 2010). Although we cannot rule out the possibility that P. viticola lectins play a role in host recognition and cellular adhesion, the low level of expression found for one of the P. viticola Jacalin-like lectins in infected tissue (Fig 3) suggests that, rather than being involved in the interaction with the plant, these proteins might function as storage proteins in zoospores, with putative roles in defence against pathogens in the early stages of P. viticola development.…”

Section: Discussionmentioning

confidence: 99%

Identification of effector genes from the phytopathogenic Oomycete Plasmopara viticola through the analysis of gene expression in germinated zoospores

2012

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Grapevine downy mildew caused by the Oomycete Plasmopara viticola is one of the most important diseases affecting Vitis spp. The current strategy of control relies on chemical fungicides. An alternative to the use of fungicides is using downy mildew resistant varieties, which is cost-effective and environmentally friendly. Knowledge about the genetic basis of the resistance to P. viticola has progressed in the recent years, but little data are available about P. viticola genetics, in particular concerning the nature of its avirulence genes. Identifying pathogen effectors as putative avirulence genes is a necessary step in order to understand the biology of the interaction. It is also important in order to select the most efficient combination of resistance genes in a strategy of pyramiding. On the basis of knowledge from other Oomycetes, P. viticola effectors can be identified by using a candidate gene strategy based on data mining of genomic resources. In this paper we describe the development of Expressed Sequence Tags (ESTs) from P. viticola by creating a cDNA library from in vitro germinated zoospores and the sequencing of 1543 clones. We present 563 putative nuclear P. viticola unigenes. Sequence analysis reveals 54 ESTs from putative secreted hydrolytic enzymes and effectors, showing the suitability of this material for the analysis of the P. viticola secretome and identification of effector genes. Next generation sequencing of cDNA from in vitro germinated zoospores should result in the identification of numerous candidate avirulence genes in the grapevine/downy mildew interaction.

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Entomotoxic effects of fungal lectin from Rhizoctonia solani towards Spodoptera littoralis

Cited by 41 publications

References 33 publications

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Antifungal Action of NewTrichoderma Spp.Romanian Isolates on Different Plant Pathogens

Identification of effector genes from the phytopathogenic Oomycete Plasmopara viticola through the analysis of gene expression in germinated zoospores

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