Entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) screening for lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) control
Abstract:Alphitobius diaperinus (Coleoptera: Tenebrionidae), an important poultry insect pest, is difficult because of the insect’s behavior and biology and the restrictions for the used of synthetic insecticides. So, entomopathogenic nematodes are an alternative for A. diaperinus control. This study aimed to screen in laboratory entomopathogenic nematodes for A. diaperinus control. Fourteen strains were tested, and for the best LC50 and LC90 was estimated, as well as the control efficiency on adult insects at differen… Show more
“…The isolate was previously selected as the most virulent for G. spegazziniana (Formentini et al 2015). Conidia were obtained from a culture medium (ME) (10 days, 26 ± 1 °C, 14:10 light:dark photoperiod) (Alves et al 1998). The conidia were collected by scraping the medium surface and then transferred to a flask with a 0.05% Tween 80 solution in distilled water.…”
An ‘attract-and-infect’ device for controlling adult yerba mate ampoule Gyropsylla spegazziniana (Lizer & Trelles, 1919) (Hemiptera: Aphalaridae) was designed and tested in the lab. The device was constructed from plastic sheets containing small, recessed wells. The wells were filled with liquid culture medium and inoculated with conidia of Beauveria bassiana (Bals.-Criv.) Vuill.. After incubation, the devices were hung in cages containing a yerba mate seedling and adults of G. spegazziniana. For comparison, yellow cardboard cards impregnated with conidia were used. There were 28 times more conidia in plastic device than in the yellow card. Conidia had a germination level above 90% and infected approximately 56% of the insects. Further tests in the field are planned to evaluate its effectiveness for managing populations of G. spegazziniana in yerba mate.
“…The isolate was previously selected as the most virulent for G. spegazziniana (Formentini et al 2015). Conidia were obtained from a culture medium (ME) (10 days, 26 ± 1 °C, 14:10 light:dark photoperiod) (Alves et al 1998). The conidia were collected by scraping the medium surface and then transferred to a flask with a 0.05% Tween 80 solution in distilled water.…”
An ‘attract-and-infect’ device for controlling adult yerba mate ampoule Gyropsylla spegazziniana (Lizer & Trelles, 1919) (Hemiptera: Aphalaridae) was designed and tested in the lab. The device was constructed from plastic sheets containing small, recessed wells. The wells were filled with liquid culture medium and inoculated with conidia of Beauveria bassiana (Bals.-Criv.) Vuill.. After incubation, the devices were hung in cages containing a yerba mate seedling and adults of G. spegazziniana. For comparison, yellow cardboard cards impregnated with conidia were used. There were 28 times more conidia in plastic device than in the yellow card. Conidia had a germination level above 90% and infected approximately 56% of the insects. Further tests in the field are planned to evaluate its effectiveness for managing populations of G. spegazziniana in yerba mate.
“…The two most important and studied families are Steinernematidae and Heterorhabditidae, these are associated with bacteria of the genera Xenorhabdus and Photorhabdus, respectively (Vashisth et al, 2013). Different strains of EPN have been used as biological control agents for various pests, mainly in the agricultural sector for the protection of various crops (Parada et al, 2019), and in pests of livestock production animals (Alves et al, 2012), although it is necessary to deepen its study in the livestock sector, as well as the effect that the chemical products used for animals have on the populations of these nematodes (Negrete et al, 2018).…”
Objective: to determine the effect of coumaphos on Rhipicephalus microplus and entomopathogenic nematodes in bovine production units.
Design/methodology/approach: Two experiments were carried out: First) tick samples were collected and evaluated, using the Adult Immersion Test (10 ticks per humid chamber), the treatments were applied: 1) Control; 2) Coumaphos 0.1 %; 3) Coumaphos 0.2 %; 4) Coumaphos 0.4 %. An ANOVA was carried out with a completely randomized design (mortality dependent variable and treatment independent variable with four levels) and a multiple comparison of means (Tukey's test). Second experiment) 10 samples of livestock soil where coumaphos is used, which correspond to the Mariano Matamoros ejido, Tamaulipas, and 10 non-livestock soil from the Las Huastecas Experimental Field, were analyzed to determine the presence of entomopathogenic nematodes (EPN). a cross-frequency table between the sampled soils and the Chi-square test.
Results: Experiment 1) In counting the tick mortality percentages on the second day after application in group 1) control was 37.5 %, 2) 72.5 %, 3) 80 %, 4) 92.5 %, on day 7 and day 8 there was no difference with 90 % and 100 % mortality. Experiment 2) The soils positive to EPN were 35 % Non-livestock and 25 % Livestock.
Limitations on study/implications: it is important to make producers aware of the correct use of chemical products.
Findings/conclusions: R. Microplus was susceptible to coumaphos in cattle production units. A greater presence of EPN was found in non-livestock soils, which may be related to the use of chemical products to control parasites.
“…Ecofriendly tactics have been sought, including botanical insecticides, biocontrol with nematodes and entomopathogenic fungi, heat, alcoholic solution, gas (ozone and ammonium), and diatomaceous earth (DE) (ALVES et al, 2006(ALVES et al, , 2012(ALVES et al, , 2015FOGAÇA et al, 2017;OLIVEIRA et al, 2017;GEHRING et al, 2019).…”
The lesser mealworm (LMW) is the most important pest in poultry production. Insects are associated to avian pathogens, mainly Salmonella. Its control is based on chemical insecticide, with limited efficacy in population reduction. Also, insect populations are resistant to the main active ingredients used. Diatomaceous earth (DE) is a mineral dust that has activity against the LMW when used in dust application. No information is available about DE liquid preparation against this insect. Thus, bioassays were conducted aiming to develop a strategy for DE liquid preparation to this insect control. In laboratory the ideal concentration for poultry house experiment was estimated and the effect of insect contact with poultry litter or chicken feed after application was checked. In the poultry house, DE liquid preparation (10% in water, 1 L•m -2 ) was applied on the dirt soil of a cleaned and empty poultry house. In the control poultry house, a chemical insecticide was used (cypermethrin 15 g + chlorpyriphos 25 g + piperonyl butoxide 15 g) (1 L•m -2 ). DE at all concentrations was efficient mainly when insects were dried for 24 h before contact with a substrate. In poultry house, both treatments obtained similar results at 15 days after treatment (94% of reduction of insect population). At 45 days after treatment, the insect population with DE treatment increased 39% while the insect population remained 17% smaller than the initial population with a chemical insecticide. DE liquid preparation has potential to be used as a safe treatment in LMW population management, as a shock treatment.
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