Entomopathogenic fungi and their biological control of Tetranychus urticae: Two-spotted spider mites

Khamis Al-Zahrani, Jawaher; Hassan Al-Abdalall, Amira; Aly Osman, Mohamed; Aldakheel, Lena A.; Faisal AlAhmady, Nada; Aldakeel, Sumayh A.; AbdulAzeez, Sayed; Francis Borgio, J.; ElNaggar, Medhat A.; Alabdallah, Nadiyah M.; Almustafa, Mona M.

doi:10.1016/j.jksus.2023.102910

Cited by 3 publications

(5 citation statements)

References 30 publications

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“…Elhakim et al [52] observed higher protease activity in the M. robertsii isolate compared to the B. bassiana isolate, which was correlated with the elevated virulence of the former strain against T. urticae. Furthermore, in a recent study, Khamis Al-Zahrani et al [37] found that the activity of protease in B. bassiana was the lowest vs. other fungi belonging to the genera Fusarium, Aspergillus, Scopulariopsis, and Penicillium after a screening performed on skimmed milk agar medium. All EPFs were obtained from Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) with the exception of one Fusarium isolate obtained from T. urticae.…”

Section: Discussionmentioning

confidence: 99%

“…Then, discs with the mites were sprayed with 1 mL [81] of the suspension (1 × 10 8 conidia/mL) of each isolate, using an airbrush (Master Multi-purpose Airbrush, USA). Control leaves were sprayed with 1 mL of H 2 O [37]. After spraying, leaves were left to air-dry for about 300 sec [82].…”

Section: Direct Action Of Beauveria Bassiana and Metarhizium Robertsi...mentioning

confidence: 99%

“…Mites were considered dead if they could not move their appendages when touched with a camel hair brush [82]. The dead mites were placed on new plates containing a wet filter paper and stored in the same conditions for 3 days to observe any fungal growth to confirm their mortality caused by EPF [37,84]. The experiment was repeated four times using new plant leaves (i.e., 3 subreplications × 4 isolates and control × 4 replications).…”

Section: Direct Action Of Beauveria Bassiana and Metarhizium Robertsi...mentioning

confidence: 99%

“…Totally, T. urticae is resistant to 96 active ingredients in 526 cases; therefore, it is considered one of the most resistant pests among the arthropods [35]. Several factors contribute to the rapid development of acaricide resistance in T. urticae, such as the ability to reproduce by arrhenotokous parthenogenesis, large number of offspring, and short life cycle [36,37].…”

Section: Introductionmentioning

confidence: 99%

“…In a biological control strategy, EPFs could replace conventional acaricides [41] or act synergistically with other biocontrol agents, such as predatory mites [42][43][44]. In addition, EPFs are not detrimental to non-target organisms or the environment, and are safe for humans [37]. Fungi are usually more advantageous over other microbial biopesticides (e.g., viruses and nematodes) because of their broader host selectivity since one isolate can attack and control different arthropod pests simultaneously [45][46][47][48].…”

Section: Introductionmentioning

confidence: 99%

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Impact of Three Entomopathogenic Fungal Isolates on the Growth of Tomato Plants—Ectoapplication to Explore Their Effect on Tetranychus urticae

Wakil,

Boukouvala,

Kavallieratos

et al. 2024

Agronomy

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Tetranychus urticae is an important pest of tomato crops globally, affecting plant yield and growth. Beauveria bassiana and Metarhizium robertsii have the potential to control T. urticae. We investigated the influence of two B. bassiana (i.e., WG-12 and WG-19) isolates and one M. robertsii (WG-02) isolate when colonizing different plant organs (leaves, stems, and roots) and their influence on the growth of tomato plants, through foliar, root-dipping, and seed-soaking application techniques. We also examined the acaricidal activity of the three isolates against T. urticae (female adults), spraying tomato leaf discs with each isolate separately. After 28 days, WG-12 and WG-19 colonized 97 and 91% of the leaves after foliar inoculation, whereas WG-02 exhibited the lowest leaf colonization (76%). The height of the tomato plants, the root length, the number of leaves, and the weight of the biomass above and below the ground were enhanced significantly after inoculation with WG-02 vs. B. bassiana isolates and control. The complete mortality of T. urticae was caused by WG-12 and WG-02 after 10 days, whereas WG-19 killed 94% of the adults. For the effective management of T. urticae, we propose the application of the WG-02 isolate since it provides complete protection and promotes the growth of tomato plants.

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Section: Discussionmentioning

confidence: 99%

Section: Direct Action Of Beauveria Bassiana and Metarhizium Robertsi...mentioning

confidence: 99%

Section: Direct Action Of Beauveria Bassiana and Metarhizium Robertsi...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impact of Three Entomopathogenic Fungal Isolates on the Growth of Tomato Plants—Ectoapplication to Explore Their Effect on Tetranychus urticae

Wakil,

Boukouvala,

Kavallieratos

et al. 2024

Agronomy

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Acaricidal Efficacy of Abamectin against Tetranychus urticae Populations When Combined with Entomopathogenic Fungi

Wakil,

Boukouvala,

Kavallieratos

et al. 2024

Horticulturae

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Tetranychus urticae (Acari: Tetranychidae) is a widespread and serious mite pest that infests tomato plants and causes economic losses worldwide. We investigated the acaricidal efficacy of two isolates of entomopathogenic fungi (EPF) Metarhizium robertsii (WG-7) and Beauveria bassiana (WG-12) alone and in combination with abamectin when applied topically to tomato leaf discs in the laboratory against T. urticae. We also evaluated the establishment and proliferation of T. urticae mite life stages on tomato plants in the greenhouse after application of each of the above treatments. The combination of abamectin with each EPF caused 100% mortality in T. urticae immatures after 2 days while each EPF or abamectin alone caused moderate mortality, not exceeding 74.2% 3 days post-exposure. Complete (100%) mortality of adults was observed after 5 days in leaf discs treated with M. robertsii plus abamectin whereas B. bassiana plus abamectin caused 100% mortality after 7 days. The mean number of eggs, emerged immatures, and adults were significantly reduced on both sides of the leaves (i.e., abaxial and adaxial sides) after using the combined application of M. robertsii or B. bassiana plus abamectin, compared to abamectin alone and controls. Our results reveal that the acaricidal efficacy of abamectin combined with either EPF was significantly better in managing the T. urticae life stages than either treatment alone under greenhouse conditions.

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Beauveria bassiana Vuillemin İzolatlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerine Ovisidal Etkisi

Uysal,

Obut,

Uzun Yiğit

2024

Afyon Kocatepe University Journal of Sciences and Engineering

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Bu çalışma ile Beauveria bassiana’nın BY2 (Burdur, Yeşilova), BIM-001 (Isparta, Merkez) ve IGÇ (Isparta, Merkez) izolatlarının üç farklı dozunun (1x106 spor/ml, 1x107 spor/ml ve 1x108 spor/ml) Tetranychus urticae üzerinde ovisidal etkisinin laboratuvar koşullarında belirlenmesi hedeflenmiştir. Ticari preparat Nostalgist (Agrobest Grup Ltd. Şti, Beauveria bassiana Bb-1 ırkı) ve kontrol olarak Tween 80 (% 0,1) içeren saf su kullanılmıştır. Denemeler plastik petrilerde (9 cm) 5 tekerrürlü olarak yürütülmüştür. Fasulye yaprak diskleri (4 cm) petri plakasında tutulan steril suya doymuş pamuk üzerinde yerleştirilmiştir. Daha sonra 10 ergin birey, yaprak disklere aktarılarak yumurta bırakmalarına izin verilmiştir. Her bir petri plakasında 20 yumurta/yaprak diski olacak şekilde ayarlanmıştır. Her bir entomopatojen fungus izolatına ait spor süspansiyonu 4 bar basınçta püskürtme sağlayan modifiye edilmiş bir düzenek yardımıyla 10 sn süre ile yumurta içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak yumurta ölümleri kaydedilmiştir. Sonuç olarak, B. bassiana BIM-001, BY2 ve IGÇ izolatlarının 1x108 spor/ml konsantrasyonları uygulandıktan 7 gün sonra T. urticae yumurtalarında meydana gelen ölüm oranları sırasıyla %71, %66 ve %59 olarak belirlenmiştir. Beauveria bassiana strain Bb-1 uygulaması diğer entomopatojen fungus izolatlarına ait uygulamalardan istatistiksel olarak anlamlı bulunmuştur. Bununla birlikte B. bassiana BIM-001 1x108, 1x107 spor/ml ve B. bassiana BY2 1x108 spor/ml uygulamalarındaki ölüm oranları arasında fark olmadığı saptanmıştır (p˂0.005).

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Entomopathogenic fungi and their biological control of Tetranychus urticae: Two-spotted spider mites

Cited by 3 publications

References 30 publications

Impact of Three Entomopathogenic Fungal Isolates on the Growth of Tomato Plants—Ectoapplication to Explore Their Effect on Tetranychus urticae

Impact of Three Entomopathogenic Fungal Isolates on the Growth of Tomato Plants—Ectoapplication to Explore Their Effect on Tetranychus urticae

Acaricidal Efficacy of Abamectin against Tetranychus urticae Populations When Combined with Entomopathogenic Fungi

Beauveria bassiana Vuillemin İzolatlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerine Ovisidal Etkisi

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