Entirely recombinant protein-based hydrogels for selective heavy metal sequestration

Kou, Songzi; Yang, Zhongguang; Luo, Jiren; Sun, Fei

doi:10.1039/c7py01206c

Cited by 31 publications

(30 citation statements)

References 32 publications

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“…Therefore, the uranyl ions can easily immigrate into the inner layer of the hydrogel . While other hydrogel‐based adsorbents have high uranium adsorption capacities or good selectivity, amidoxime‐functionalized hydrogels have both . However, to improve the hydrophilicity of the poly(amidoxime) (PAO) and other amidoxime‐functionalized polymers, additional hydrophilic hydrogel 3D networks are commonly introduced.…”

Section: Methodsmentioning

confidence: 99%

“…However, the proven uranium ore reserves on land are limited, and can only provide for about 70 years of consumption of global nuclear energy. [2] To efficiently recover uranium from seawater, for the long-term sustainable development of nuclear power, many types of uranium adsorbents have been developed, such as inorganic materials, [3][4][5] synthetic organic and polymeric materials, [6][7][8] various nanostructured adsorbents [9][10][11][12][13][14][15] including grafted polymeric porous supports, [9] metal-organic www.advmat.de www.advancedsciencenews.com or good selectivity, [18,40] amidoxime-functionalized hydrogels have both. [41,42] However, to improve the hydrophilicity of the poly(amidoxime) (PAO) and other amidoxime-functionalized polymers, additional hydrophilic hydrogel 3D networks are commonly introduced.…”

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confidence: 99%

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An Ion‐Crosslinked Supramolecular Hydrogel for Ultrahigh and Fast Uranium Recovery from Seawater

et al. 2020

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Large‐scale uranium extraction from seawater is a crucial but challenging part of nuclear power generation. In this study, a new ion‐crosslinked supramolecular Zn2+–poly(amidoxime) (PAO) hydrogel that can super‐efficiently adsorb uranium from seawater is explored. By simply mixing two solutions of zinc chloride and PAO, a supramolecular Zn2+–PAO hydrogel is achieved via the interaction between zinc cations and amidoxime anions. In contrast with existing amidoxime‐functionalized hydrogel‐based adsorbents having low PAO contents and fiber‐based adsorbents with weak hydrophilicity, the PAOs can be directly crosslinked using a small quantity of superhydrophilic zinc ion. Thus, a supramolecular hydrogel is formed, having both a high content of well‐dispersed PAOs and good hydrophilicity. Relative to reported adsorbents, this low‐cost hydrogel membrane exhibits outstanding uranium adsorption performance, reaching 1188 mg g-1 of MU/Mdry gel in 32 ppm uranium‐spiked water. More importantly, after immersion in natural seawater for only 4 weeks, the uranium extraction capacity of the Zn2+–PAO hydrogel membrane reaches 9.23 mg g-1 of MU/Mdry gel. This work can provide a general strategy for designing a new type of supramolecular hydrogel, crosslinked by various bivalent/multivalent cation‐crosslinkers and even many other superhydrophilic supramolecular crosslinkers, for the high‐efficient and massive extraction of uranium from seawater.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

An Ion‐Crosslinked Supramolecular Hydrogel for Ultrahigh and Fast Uranium Recovery from Seawater

et al. 2020

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“…These ranged from 10 3 to 10 7 for a series of the most common metal ions found in sea water (Zhang et al, 2014), suggesting that the protein might have potential for extracting UO22+ from seawater. SUP has also subsequently been incorporated into protein hydrogels (Kou et al, 2017a,b) and 2D protein multilayers (Zhang et al, 2018) with high UO22+ adsorption capacity. These materials could be used for the decontamination of depleted uranium, often found in groundwaters in former conflict areas or for the development of highly sensitive UO22+ biosensors.…”

Section: Introductionmentioning

confidence: 99%

Probing Metal Ion Discrimination in a Protein Designed to Bind Uranyl Cation With Femtomolar Affinity

Hoarau

Koebke

Chen

et al. 2019

Front. Mol. Biosci.

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The design of metal-binding sites in proteins that combine high affinity with high selectivity for the desired metal ion remains a challenging goal. Recently, a protein designed to display femtomolar affinity for UO22+, dubbed “Super Uranyl-binding Protein” (SUP), was described, with potential applications for removing UO22+ in water. Although it discriminated most metal ions present in seawater, the protein showed a surprisingly high affinity for Cu2+ ions. Here, we have investigated Cu2+ binding to SUP using a combination of electron paramagnetic resonance, fluorescence and circular dichroism spectroscopies. Our results provide evidence for two Cu2+ binding sites on SUP that are distinct from the UO22+ binding site, but one of which interferes with UO22+ binding. They further suggest that in solution the protein's secondary structure changes significantly in response to binding UO22+; in contrast, the crystal structures of the apo- and holo-protein are almost superimposable. These results provide insights for further improving the selectivity of SUP for UO22+, paving the way toward protein-based biomaterials for decontamination and/or recovery of uranium.

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“…57 Combining Spy and Snoop pairs also allowed layer-by-layer nanoassembly to harvest uranium from seawater. 58,59 To address the environmental impact of conventional lightemitting diode (LED) phosphors, a white LED was assembled through ligation of uorescent proteins emitting in different parts of the spectrum (Fig. 3C).…”

Section: Multiplexing Protein Functionmentioning

confidence: 99%

Power to the protein: enhancing and combining activities using the Spy toolbox

2020

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Entirely recombinant protein-based hydrogels for selective heavy metal sequestration

Abstract: Green mining and heavy metal remediation enabled by metalloprotein hydrogels.

Cited by 31 publications

References 32 publications

An Ion‐Crosslinked Supramolecular Hydrogel for Ultrahigh and Fast Uranium Recovery from Seawater

An Ion‐Crosslinked Supramolecular Hydrogel for Ultrahigh and Fast Uranium Recovery from Seawater

Probing Metal Ion Discrimination in a Protein Designed to Bind Uranyl Cation With Femtomolar Affinity

Power to the protein: enhancing and combining activities using the Spy toolbox

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