Enterovirulent E. coli in inflammatory and noninflammatory bowel diseases

Čurová, Katarína; Kmeťová, M; Sabol, Martin; Gombošová, Laura; Lazúrová, Ivica; Siegfried, Leonard

doi:10.1007/s12223-009-0012-y

Cited by 15 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Template DNA was extracted by the boiling-water method [17]. All E. coli strains were screened for presence of genes encoding toxins by a simplex and multiplex PCR assays using specific primers in appropriate conditions (Table 1).…”

Section: Virulence Factor Genesmentioning

confidence: 99%

Toxins of Extraintestinal Escherichia coli Isolated from Blood Culture

Čurová

KmeÅ¥ová²,

Vargová³

et al. 2014

Clin Microbiol

View full text Add to dashboard Cite

Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the main etiological agents of bloodstream infections caused by Gram-negative bacilli. ExPEC pathogenicity is due to the presence of genes, located on plasmids or chromosomes that encode virulence factors. E. coli virulence factors such as adhesins, toxins, invasins are able to modify the metabolism of host cell, contributing to disease. In this study, 80 strains of E. coli were isolated by hemoculture from septicemic patients and examinated by polymerase chain reaction to identify the virulence factors genes encoding toxins and to determine the phylogenetic group. We characterized genes encoding 3 different types of toxins: α-hemolysin (hlyA), cytotoxic necrotizing factor type 1 (cnf1) and five subtypes of cytolethal distending toxins (cdt-I to cdt-V). 23.75% of E. coli strains contained cnf1 gene and 22.5% hlyA. Genes cdt-I and cdt-IV were detected in 1 of 80 strains. Phylogenetic classification showed that E. coli strains fall into 4 groups (A, B1, B2, D) based on PCR detection (chuA and yjaA genes and DNA fragment TSPE4.C2). Virulent ExPEC belong mostly to groups B2 and D. Our results confirmed this fact: 56% of E. coli strains belonged to group B2 and 24% to group D.

show abstract

Section: Virulence Factor Genesmentioning

confidence: 99%

Toxins of Extraintestinal Escherichia coli Isolated from Blood Culture

Čurová

KmeÅ¥ová²,

Vargová³

et al. 2014

Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…EIEC and STEC were not found in collected samples. 17 Moreover, EAEC was detected in 2.5%, EPEC in 1.3% and ETEC in 1.5% of all IBD E. coli isolates in the study of Cǔrová et al The authors concluded that their low incidence was in agreement with the fact that they are associated with diarrhea, not to other GIT diseases, 18 while other studies found that all E. coli IBD isolates were negative for intestinal genes. 5,6 They justified the very low detection rate of classical E. coli intestinal genes by the previous observations confirming that E. coli from IBD patients are more closely related to ExPEC.…”

Section: Antimicrobial Susceptibility Resultsmentioning

confidence: 89%

Phylogenetic and pathotype analysis of Escherichia coli stool isolates from Egyptian patients with inflammatory bowel disease

Meheissen¹,

Header²,

Abdelaty³

2019

Germs

View full text Add to dashboard Cite

Introduction The role of Escherichia coli in the pathogenesis of inflammatory bowel disease (IBD) is still controversial. The study aimed to investigate the pathotypes and the phylogenetic groups of E. coli in Egyptian patients with IBD in an attempt to find an association between any type or group with the severity of the disease. Methods Thirty ulcerative colitis (UC), 30 Crohn's disease (CD), and 20 control subjects with normal colonoscopy were included in a cross-sectional study. E. coli were isolated from stool samples by culture. Eight intestinal virulence genes coding for diarrheagenic E. coli were investigated using multiplex PCR. Phylogenetic grouping was performed by a triplex PCR. Antimicrobial susceptibility of all isolates was done using disc diffusion method. Results Enteroaggregative E. coli (EAEC) were identified in 25% (15/60) of IBD cases and in none of the controls (p=0.013). Out the 60 IBD cases, 30 (50%) were from phylogenetic group B2. No statistically significant differences in the distribution of E. coli phylogenetic groups were found between study groups. However, 80% of EAEC were assigned to group B2 and D. No statistically significant differences in calprotectin level or in disease severity scores were reported between the four phylogenetic groups. E. coli from both UC and CD patients showed a high rate of resistance to most antimicrobials when compared to the control group. Conclusions The identification of EAEC belonging mainly to group B2 and D in IBD cases may indicate the importance of this pathotype in the pathogenesis of IBD in Egyptian patients. Keywords E. coli, enteroaggregative, IBD, phylogenetic group. Introduction1 Inflammatory bowel disease (IBD) constitutes a group of inflammatory gastrointestinal relapsing and remitting clinical conditions, of which Crohn's disease (CD) and ulcerative colitis (UC) are the most common forms. The pathogenesis of

show abstract

“…Malignancy gene profiles are inextricably related to the evolutionary origin of the strain 47 . Where B2 and D are predominant in patients with IBD, more commonly discovered virulence-linked genes indicative of ExPEC were found in IBD patients than in controls, depending on the abundance of phylogenetic groups, and without differences in other investigation types 50 . A shift in the phylotype distribution would result in increased abundance of E. coli with colonization factors, facilitating fixation and persistence in IBD patients.…”

Section: Pathogenic Properties Of E Colimentioning

confidence: 93%

“…Although inspecific genetic characteristics separate E. coli from the UC or CD gut mucosa, certain virulence factors have been discovered that are distributed variably amongst these IBD types. For instance, diarrhea-linked hemolytic E. coli, also known as cell-detaching E. coli (CDEC), has been detected in about 24 % of UC E. coli patients, while detected in only 4.7 % of CD E. coli patients 50 . Diarrhea-linked hemolytic E. coli usually carries pilus P, hemolysin, S-fimbria genes, and cytotoxic necrotizing factor 1.…”

Section: Pathogenic Properties Of E Colimentioning

confidence: 99%

“…Diarrhea-linked hemolytic E. coli usually carries pilus P, hemolysin, S-fimbria genes, and cytotoxic necrotizing factor 1. The uropathogenic-specific protein (USP) gene, which codes for the uropathogenic-specific protein discovered in UC patient E. coli, is more abundant than in CD patient E. coli 50 . The E. coli containing iro gene, which codes for an iron-chelating siderophores receptor, was newly seen to be more very often separated from inflammatory and uninflamed mucosa-inactive patients with UC.…”

Section: Pathogenic Properties Of E Colimentioning

confidence: 99%

See 1 more Smart Citation

Escherichia coli Strains in Patients with Inflammatory Bowel Diseases: A Review

Al-Amrah

AL-Otaibi

Sadiq

2022

Biosci., Biotech. Res. Asia

View full text Add to dashboard Cite

Gastrointestinal tract conditions, including inflammatory bowel diseases (IBDs) such as ulcerative colitis (UC) and Crohn’s disease, have been linked to adhesive invasive Escherichia coli (AIEC) pathotypes, with comparable pathogenic properties, although the incidence of AIEC with UC and CD is generally undetermined. While a significant advance has been made in understanding the pathogenic processes of AIEC since it was first characterized a decade ago, the molecular basis that determines the phenotypic features of AIEC pathotypes is still unknown. This article reviews studies that examine the prevalence of E. coli in patients with IBD and discusses its pathophysiological role.

show abstract

Enterovirulent E. coli in inflammatory and noninflammatory bowel diseases

Cited by 15 publications

References 31 publications

Toxins of Extraintestinal Escherichia coli Isolated from Blood Culture

Toxins of Extraintestinal Escherichia coli Isolated from Blood Culture

Phylogenetic and pathotype analysis of Escherichia coli stool isolates from Egyptian patients with inflammatory bowel disease

Escherichia coli Strains in Patients with Inflammatory Bowel Diseases: A Review

Contact Info

Product

Resources

About