Enteroviral meningitis in children in Turkey

Şensoy, Gülnar; Sel, Kutay; Ozkaya, E.; Çakır, Burçin; Vidinlisan, Sadi; Doğancı, Levent

doi:10.2478/s11536-008-0055-5

Cited by 6 publications

(13 citation statements)

References 33 publications

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“…These viruses are transmitted via oral-fecal route and mostly seen in fall and summer at temperate areas (4). Echovirus 30 as a member of enteroviruses was reported from some countries such as Kuwait, Turkey, Brazil, and Korea (2,(5)(6)(7). Cell culture was the gold standard and classical diagnostic method for detection and isolation of enteroviruses but as this method is very time consuming and needs much facilities, after the advent of molecular techniques such as PCR most studies are performed based on these techniques (8).…”

Section: Introductionmentioning

confidence: 99%

Relative Frequency of Echovirus 30 in Patients Suffering from Enterovirus Meningitis in Ahvaz

Rasti

Samarbafzadeh

Makvandi

et al. 2013

Jundishapur J Microbiol

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Background: Human enteroviruses are members of Picornaviridae family; they are non-enveloped, icosahedral viruses with positive RNA as genome. Echovirus 30 is an important member of enteroviruses that is recognized in outbreaks of enterovirus meningitis. Objectives: The aim of this study was to determine relative frequency of echovirus 30 as an important agent of aseptic meningitis among children referred to Aboozar hospital, Ahvaz, Iran. Patients and Methods: 34 cerebrospinal fluid samples from patients with enterovirus aseptic meningitis, negative bacterial culture, WBC (white blood cell) count > 5x106/mm3, and aseptic meningitis symptoms were entered in the study. These samples were collected in a year between May 2010 and May 2011. RNA of enteroviruses were extracted and investigated for echovirus 30 infection with RT-PCR (reverse transcription polymerase chain reaction) test. Samples also were cultured in RD (rhabdomyosarcoma) cell and positive results were approved by RT-PCR test with enterovirus specific primers. To recognize PCR-product, 440 bp RT-PCR product was sequenced and phylogenic tree was drawn based on Neighbor Joining method with 1000 replication bootstrap. Results: Echovirus 30 infection was not detected in any case. Just one CSF sample grew in RD cell culture. This sample was approved by RT-PCR and sequencing. Positive sample was recognized as coxsackie virus B3. Conclusions: There was no echovirus 30 in Ahvaz because of diverse nature of enteroviruses and several serotypes with various distribution patterns in different geographical regions, and the fact that echovirus 30 is mostly detected in outbreaks rather than endemism. Coxsackie virus B3 was responsible for aseptic meningitis of a child in this study. Based on another study conducted in Tehran, it seems that Coxsackie B viruses are among current agents causing enterovirus aseptic meningitis in Iran. Of course we need to conduct more studies in Ahvaz and other parts of the country to approve this hypothesis.

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Section: Introductionmentioning

confidence: 99%

Relative Frequency of Echovirus 30 in Patients Suffering from Enterovirus Meningitis in Ahvaz

Rasti

Samarbafzadeh

Makvandi

et al. 2013

Jundishapur J Microbiol

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“…In previous studies it has been reported that central nervous system infections are more common in men. Michos et al (13) reported M/F ratio in bacterial meningitis as 1.79, while Harald et al (14) found the M/F ratios as 1.33 in bacterial, and 1.84 in aseptic meningitis and in aseptic meningitis, respectively Şensoy et al (12) , and Ceyhan et al (10) from our country reported M/F ratios as 2.05, and 1.55 in aseptic meningitis, respectively. In our study, the M/F ratio was 1.78 in bacterial, and 0.37, in aseptic meningitis.…”

Section: Discussionmentioning

confidence: 67%

“…Taşkesen et al reported the average length of hospital stay as 10.5 days (22) . Michos et al (13) indicated the average length of hospitalization for aseptic meningitis as four days, Şensoy et al (12) stated the average length of hospital stay as 6.2 days for aseptic meningitis. Although there is no statistical difference between the groups in terms of length of hospital stay, it is noteworthy that the duration of hospitalization of patients with bacterial meningitis (average 10 days) was shorter than that of patients with aseptic meningitis (average 14.3 days).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Meningitis Cases in Pediatric Intensive Care Unit: 8-Year Single Center Experience

Hatipoğlu¹,

Şevketoğlu²

2020

BMJ

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Objective: The aim of the study was to evaluate clinical features, management of intensive care treatment, complications and mortality in children with meningitis treated in pediatric intensive care unit. Method: Data of 47 patients who were followed-up in the pediatric intensive care unit between January 2006 and December 2014 with the diagnosis of meningitis were retrospectively reviewed. Patients were screened for demographic features, etiological and predisposing factors, physical examination and laboratory findings, treatment, prognosis, mortality rate, acute and chronic complications, Glasgow coma scale and pediatric mortality risk score. Results: The age, sex distribution, hospitalization day, and chronic disease rate of aseptic meningitis and bacterial meningitis did not differ significantly. In bacterial meningitis, S. pneumoniae was detected in 12.5% of patients and N. meningitidis in 6.2% of patients. In aseptic meningitis, invasive ventilation, inotropic use rate and pediatric mortality risk score score were significantly lower than the bacterial meningitis (p=0.011, p=0.042 and p=0.043, respectively). Conclusion: Early diagnosis, initiation of appropriate antibiotic therapy, and provision of necessary life support in life-threatening complications may reduce the need for pediatric intensive care unit, morbidity and mortality in meningitis patients. We believe that multicenter and prospective studies are needed to reduce sequelae and mortality of meningitis cases observed in pediatric intensive care unit.

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“…They may cause several diseases such as conjunctivitis, upper and lower respiratory tract infections, meningitis, encephalitis, encephalomyelitis and acute flaccid paralysis [3,7,13]. …”

Section: Discussionmentioning

confidence: 99%

Improving the diagnosis of meningitis due to enterovirus and herpes simplex virus I and II in a tertiary care hospital

et al. 2013

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BackgroundEnterovirus and herpes simplex viruses are common causes of lymphocytic meningitis. The purpose of this study was to analyse the impact of the use molecular testing for Enteroviruses and Herpes simplex viruses I and II in all suspected cases of viral meningitis.MethodsFrom November 18, 2008 to November 17, 2009 (phase II, intervention), all patients admitted with suspected viral meningitis (with pleocytosis) had a CSF sample tested using a nucleic acid amplification test (NAAT). Data collected during this period were compared to those from the previous one-year period, i.e. November 18, 2007 to November 17, 2008 (phase I, observational), when such tests were available but not routinely used.ResultsIn total, 2,536 CSF samples were assessed, of which 1,264 were from phase I, and 1,272 from phase II. Of this total, a NAAT for Enterovirus was ordered in 123 cases during phase I (9.7% of the total phase I sample) and in 221 cases in phase II (17.4% of the total phase II sample). From these, Enterovirus was confirmed in 35 (28.5%, 35/123) patients during phase I and 71 (32.1%, 71/221) patients during phase II (p = 0.107). The rate of diagnosis of meningitis by HSV I and II did not differ between the groups (13 patients, 6.5% in phase I and 13, 4.7% in phase II) (p = 1.0), from 200 cases in phase I and 274 cases in phase II.ConclusionsThe number of cases diagnosed with enteroviral meningitis increased during the course of this study, leading us to believe that the strategy of performing NAAT for Enterovirus on every CSF sample with pleocytosis is fully justified.

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Enteroviral meningitis in children in Turkey

Cited by 6 publications

References 33 publications

Relative Frequency of Echovirus 30 in Patients Suffering from Enterovirus Meningitis in Ahvaz

Relative Frequency of Echovirus 30 in Patients Suffering from Enterovirus Meningitis in Ahvaz

Analysis of Meningitis Cases in Pediatric Intensive Care Unit: 8-Year Single Center Experience

Improving the diagnosis of meningitis due to enterovirus and herpes simplex virus I and II in a tertiary care hospital

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