Enterotoxigenicity and Antibiotic Resistance of Coagulase-Negative Staphylococci Isolated from Raw Buffalo and Cow Milk

Osman, Kamelia M.; Pires, Állan da Silva; Franco, Octávio Luiz; Orabi, Ahmed; Hanafy, Mai H; Marzouk, Eman; Hussien, Heba; Alzaben, Feras; Almuzaini, Abdulaziz M.; Elbehiry, Ayman

doi:10.1089/mdr.2019.0114

Cited by 13 publications

(7 citation statements)

References 49 publications

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“…The number of reports on the enterotoxigenicity of CoNS has been increasing. The method most commonly used for determining the presence of enterotoxin genes is PCR, allowing the detection of small copy numbers of a given gene [ 9 , 10 , 11 , 12 ]. However, there are insufficient data about the structure of genetic elements containing enterotoxin genes in the genomes of CoNS and the expression of these genes.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcal Enterotoxin Genes in Coagulase-Negative Staphylococci—Stability, Expression, and Genomic Context

Banaszkiewicz

Wałecka-Zacharska

Schubert

et al. 2022

IJMS

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In the current study, we screened a collection of coagulase-negative staphylococci (CoNS) isolates for orthologues of staphylococcal enterotoxins (SEs) involved in S. aureus-related staphylococcal food poisoning (SFP). The amplicons corresponding to SEs were detected in S. chromogenes, S. epidermidis, S. haemolyticus, S. borealis, S. pasteuri, S. saprophyticus, S. vitulinus, S. warneri, and S. xylosus. All amplicons were sequenced and identified as parts of known S. aureus or S. epidermidis SE genes. Quantitative real-time PCR allowed determining the relative copy number of each SE amplicon. A significant portion of the amplicons of the sea, seb, sec, and seh genes occurred at low copy numbers. Only the amplicons of the sec gene identified in three isolates of S. epidermidis displayed relative copy numbers comparable to sec in the reference enterotoxigenic S. aureus and S. epidermidis strains. Consecutive passages in microbiological media of selected CoNS isolates carrying low copy numbers of sea, seb, sec, and seh genes resulted in a decrease of gene copy number. S. epidermidis isolates harbored a high copy number of sec, which remained stable over the passages. We demonstrated that enterotoxin genes may occur at highly variable copy numbers in CoNS. However, we could identify enterotoxin genes only in whole-genome sequences of CoNS carrying them in a stable form at high copy numbers. Only those enterotoxins were expressed at the protein level. Our results indicate that PCR-based detection of enterotoxin genes in CoNS should always require an additional control, like analysis of their presence in the bacterial genome. We also demonstrate S. epidermidis as a CoNS species harboring SE genes in a stable form at a specific chromosome site and expressing them as a protein.

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Section: Introductionmentioning

confidence: 99%

Staphylococcal Enterotoxin Genes in Coagulase-Negative Staphylococci—Stability, Expression, and Genomic Context

Banaszkiewicz

Wałecka-Zacharska

Schubert

et al. 2022

IJMS

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“…Staphylococcal enterotoxins are primarily responsible for food poisoning caused by Staphylococcus aureus, often found in milk and dairy products [36,37]. Pasteurization effectively reduces the concentration of microorganisms in milk and, consequently, in its derivatives.…”

Section: Microbiological and Toxicological Characterizationsmentioning

confidence: 99%

“…Pasteurization effectively reduces the concentration of microorganisms in milk and, consequently, in its derivatives. Despite this, the detection of staphylococcal enterotoxins is necessary as it is thermoresistant and can cause outbreaks of food poisoning even in the absence of the pathogen [37,38].…”

Section: Microbiological and Toxicological Characterizationsmentioning

confidence: 99%

Elaboration and Characterization of Dulce de Leche with Reduced Sugar Content

Moebus,

Pinto,

Köptcke

et al. 2023

Dairy

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Dulce de leche is a South American traditional dairy product. However, due to the high sugar content, it is unsafe for consumers with special physiological conditions, such as diabetes. Light foods were developed to meet those demands whilst maintaining the sensory characteristics. The present study aimed to develop and characterize a formulation of light dulce de leche, using sweeteners to substitute sucrose, and gums as thickening and stabilizing agents. The physicochemical assays follow the AOAC manual, and the microbiological parameters were set according to MERCOSUL’s legislation. Thirty potential consumers perform the nine-point hedonic scale for product acceptance and the seven-point scale for buying intention. Compared to the traditional formulation, the product had better rheological properties, but a lighter color. All the raw materials and final products were considered safe by the recommended microbiological and toxicological standards; however, the product showed discrepancies to the physicochemical requirements. The formulation had an overall medium acceptance and low buying intention. The product had deficiencies, needing other compounds to reach the desired characteristics. Even though it leads to a more expensive final product, it is possible to adjust the product and allow access to more selective consumers or with restrictions.

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“…It is s well known that staphylococci, including methicillin-resistant strains, are an important cause of mastitis in dairy cows, ewes, buffalo and camels [ 114 , 147 , 148 ]. The bacteria are subsequently excreted into the milk without organoleptic alteration, allowing them to spread through the food chain if the milk is not treated properly [ 59 , 135 , 146 , 148 , 149 ]. MRSA has been reported in both raw and pasteurized milk from cattle and camels [ 115 , 135 , 149 , 150 ] ( Table 3 ).…”

Section: Methicillin-resistant Staphylococci (Mrs) In Animalsmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Methicillin-Resistant Staphylococci and Mammaliicoccus (MRNaS) Associated with Animals and Food Products in Arab Countries: A Review

Belhout

Elgroud

Butaye

2022

Veterinary Sciences

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The prevalence of methicillin resistance in staphylococci has been increasing globally and is currently one of the major public health concerns. In particular, treating infections caused by staphylococci with acquired antimicrobial resistance is problematic, as their treatment is more difficult. The resistance is found both in human and animal staphylococcal strains. Methicillin-resistant staphylococci (MRS) have also been increasingly reported in wildlife. In Arab countries, MRS has been detected in food producing animals and food products; however, the risk this poses is somewhat unclear, and still a significant lack of information on the trend and distribution of these pathogens in these countries, which have a specific ecosystem (desert) and traditions (Muslim culture). In this manuscript, we aim to provide an overview of the prevalence and the major MRS clonal lineages circulating in these specific countries and compare to them other situations with different ecosystems and cultures.

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Enterotoxigenicity and Antibiotic Resistance of Coagulase-Negative Staphylococci Isolated from Raw Buffalo and Cow Milk

Cited by 13 publications

References 49 publications

Staphylococcal Enterotoxin Genes in Coagulase-Negative Staphylococci—Stability, Expression, and Genomic Context

Staphylococcal Enterotoxin Genes in Coagulase-Negative Staphylococci—Stability, Expression, and Genomic Context

Elaboration and Characterization of Dulce de Leche with Reduced Sugar Content

Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Methicillin-Resistant Staphylococci and Mammaliicoccus (MRNaS) Associated with Animals and Food Products in Arab Countries: A Review

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