Enterotoxigenic Escherichia coli in a population of infants with diarrhea in Chile

Agüero, M E; L, Reyes; Prado, Valeria; Ørskov, Ida; Ørskov, Frits; Cabello, Felipe C.

doi:10.1128/jcm.22.4.576-581.1985

Cited by 33 publications

(8 citation statements)

References 45 publications

(55 reference statements)

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“…Outer membrane proteins were extracted and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1,11). Colony DNA hybridization and Southern hybridization of restriction enzyme-digested chromosomal DNA were performed as previously described (2,11,12). The DNA probe for the 36-kDa porin gene was an intragenic PvuII-PstI DNA fragment of 800 base pairs from plasmid pST13 (1).…”

Section: Methodsmentioning

confidence: 99%

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

1990

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We examined a representative collection of Salmonella typhi strains from Chile, Peru, Mexico, India, and England for the presence of several properties. Ail strains had a conserved pattern of outer membrane proteins, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The electrophoresis profiles of chromosomal DNA digested with EcoRI and PstI restriction enzymes were similar for all the strains. A conserved pattern of hybridization was observed when digested chromosomal DNA was hybridized with DNA probes for the 36-kilodalton porin, enterobactin synthesis, and enterobactin receptor genes. AUl the strains produced enterobactin but not aerobactin in bioassays. None of the strains produced heat-labile toxin, as measured by an enzyme-linked immunosorbent assay. Colony and Southern hybridizations with DNA probes for aerobactin synthesis and its receptor and heat-labile toxin genes were negative. These results indicate that S. typhi strains from different origins have similar phenotypic and genetic properties and, as has been suggested, constitute a clone.

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Section: Methodsmentioning

confidence: 99%

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

1990

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“…An initial study found that 86 % of ETEC isolated from students becoming ill when visiting Mexico produced CFA/I [21] but when a collection of ETEC from several countries and of different serotypes were tested a much lower proportion of strains were found to produce this antigen (22). Surveys of ETEC for the production of CFA/I, CFA/II and in some cases CFA/IV again gave varying results, from 79 % colonization factor positive in a small study in Chile [23], 75% in Bangladesh [16], 29% in Thailand [14,17] to 23% in travellers returning to Japan from South-East Asia [24].…”

Section: Introductionmentioning

confidence: 98%

Surveys of human enterotoxigenicEscherichia colifrom three different geographical areas for possible colonization factors

et al. 1991

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Enterotoxigenic Escherichia coli (ETEC) from Burma, central Africa (Rwanda and Zaire) and Peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (CFAs) and putative colonization factors (PCFs): CFA/I, CFA/II, which consists of three coli surface-associated (CS) antigens, CS1, CS2 and CS3, CFA/III, CFA/IV (CS4, CS5, CS6), CS7, PCFO9, PCFO159. H4, PCFO166, and CS17. The highest proportion of ETEC with identifiable colonization factors (71%) were found in the strains from Burma, which were mainly positive for CFA/I (38%), but strains producing CFA/II (4%), CFA/IV (11%), CS7 (10%), CS17 (4%), PCFO159, H4 (2%) and PCFO166 (2%) were also found. Sixty-nine percent of the ETEC from central Africa were positive for known colonization factors. While CFA/I positive strains were important (12%), a higher number of ETEC producing CFA/IV (33%) and CS17 (24%) were found. Fifty-two percent of the Peruvian strains produced identifiable colonization factors. The largest group of strains produced antigens of the CFA/IV complex (17%), while ETEC producing CFA/II (6%), CFA/III and CS6 (2%), CS7 (6%), PCFO9 (6%), PCFO166 (8%) and CS17 (7%) were also found. These surveys show that there is a considerable variation in the proportions and types of colonization factor found in different geographical areas. From 29 to 48% of the ETEC did not possess an identifiable colonization factor. These were particularly of the LT only producing type. These results have important implications for vaccine formulation.

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“…Lanes: 1, BColV15; 2, BColV136; 3, BColV118; 4, BColV125; 5, BColV128; 6, ColV-CA7 (control); 7, DColV66; 8, DColV65; 9, DColV120; 10, DColV121; 11, DColV166; 12, DColV169. Molecular size standards: 95 megadaltons, ColV2; 56.6 megadaltons, ColVi; 65 and 42.6 megadaltons, plasmids from E. coli TR438 (5,7,13).…”

Section: Resultsmentioning

confidence: 99%

Virulence-related genes in ColV plasmids of Escherichia coli isolated from human blood and intestines

et al. 1990

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DNA probes for the colicin V, traT, iss, and iu genes were used in this study of four representative ColV plasmids together with 200 Escherichia coli strains isolated from the stools of patients with diarrhea and 146 E. coli strains isolated from the blood of patients with bacteremia. The study indicated that the ColV plasmids are heterogeneous. Southern and colony hybridization analyses showed that in most of the colicin V-producing intestinal E. coli strains, the colicin V genes are located in the chromosome (14 of 16); in most of the colicin V-producing E. coli strains isolated from the blood, they are located in plasmids (18 of 22). In both intestinal and blood E. coli isolates, the traT, iss, and aerobactin receptor genes were present at similar frequencies, but the frequency of the aerobactin synthesis genes was significantly different. The aerobactin receptor gene was present in 25% of the intestinal E. coli strains that lack the aerobactin synthesis gene. In the blood isolates, the aerobactin synthesis and receptor genes were present at almost equal frequencies. Among the colicin V-producing isolates, the iss, traT, and iu genes were present in 95.5, 86.4, and 90.9% of the blood isolates and in only 68.8, 43.8, and 81.3% of the intestinal isolates, respectively. The ColV plasmids from blood isolates that were tested for the presence of traT, iss, and iu genes were homogeneous and had DNA sequences that hybridized with each of the probes. On the other hand, the two intestinal strains containing ColV genes in a plasmid were heterogeneous in regard to the carriage of these genes. The presence of ColV is not restricted to specific O types.

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Enterotoxigenic Escherichia coli in a population of infants with diarrhea in Chile

Cited by 33 publications

References 45 publications

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

Chromosomal DNA, iron-transport systems, outer membrane proteins, and enterotoxin (heat labile) production in Salmonella typhi strains

Surveys of human enterotoxigenicEscherichia colifrom three different geographical areas for possible colonization factors

Virulence-related genes in ColV plasmids of Escherichia coli isolated from human blood and intestines

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