Enterotoxigenic Escherichia coli and Other Gram-Negative Bacteria of Infantile Diarrhea: Surface Antigens, Hemagglutinins, Colonization Factor Antigen, and Loss of Enterotoxigenicity

Bäck, Erik; Möllby, R.; Kaijser, B; Stintzing, G; Wadström, Torkel; Habte, Demissie

doi:10.1093/infdis/142.3.318

Cited by 63 publications

(36 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that toxins produced by other enterobacteria may be less stable than that produced by E. coli (19). This appears not to be an absolute finding, based on our results.…”

Section: Fig 1 T I M E Course O F Enterotoxic Activities O F E Colcontrasting

confidence: 68%

Interspecific Plasmid Transfer and Modification of Heat-Stable Enterotoxin Expression by Klebsiella pneumoniae from Infants with Diarrhea

et al. 1993

View full text Add to dashboard Cite

ABSTRACT. We have previously described two heatstable enterotoxins (ST) produced by Citrobacter freundii and by Klebsiella pneumoniae. To see whether transfer of toxigenic ability may occur between different bacterial species, conjugal mating experiments were performed using strains isolated from children. Donors and recipients were incubated together. S T production by the recipients was tested by the suckling mouse assay, which is the standard biologic method for detecting ST, and also by an ELISA test. The latter is based on MAb directed to Escherichia coli 18-or 19-amino acid toxin. Citrobacter and E. coli strains producing an ELISA-positive ST were used as plasmid donors. Recipients were E. coli, Klebsiella P89, and Klebsiella AL55. The latter had been previously shown to be capable of producing an ELISA-negative ST, but had spontaneously lost this ability. After conjugation, all strains gave positive results in the suckling mouse assay. However, E. coli and Klebsiella P89 ST were positive in the ELISA test, whereas Klebsiella AL55 S T was negative. The same three strains were transformed by inserting the plasmid pSLM004 encoding an ELISA-positive ST. Klebsiella P89 and E. coli became capable of producing an ELISA-positive ST, whereas Klebsiella AL55 cells were positive in the suckling mouse assay but negative in the ELISA. To assess whether the toxigenicity acquired was itself transmissible, the new ST-producing strains were incubated with a nontoxigenic Citrobacter strain. The latter became capable of producing an ELISA-positive S T in all cases. In conclusion: 1 ) different enterobacteria may transmit ST plasmids by conjugation; 2) the ability to produce ST is acquired together with the ability to transfer it to other bacteria; and 3) selected strains of Klebsiella are capable of modifying toxin expression through a posttranslational, yet unknown, mechanism. (Pediatr Res 33: 205-208,1993) Abbreviations ST, heat-stable enterotoxin SMA, suckling mouse assay E,scherichiu coli that produce heat-stable toxins are among the most common causes of infantile diarrhea both in developing ( I) and in industrialized (2) countries.The E. coli ST 1 or STa comprises a family of low-molecularweight peptides capable of inducing diarrhea. The genes for ST 1

show abstract

“…It has been reported that toxins produced by other enterobacteria may be less stable than that produced by E. coli (19). This appears not to be an absolute finding, based on our results.…”

Section: Fig 1 T I M E Course O F Enterotoxic Activities O F E Colcontrasting

confidence: 68%

Interspecific Plasmid Transfer and Modification of Heat-Stable Enterotoxin Expression by Klebsiella pneumoniae from Infants with Diarrhea

et al. 1993

View full text Add to dashboard Cite

show abstract

“…Heat-stable enterotoxin is produced by other bacteria considered autochthonous, such as Klebsiella pneumoniae (127), Enterobacter cloacae (128), and Proteus spp. (10). Thus, certain plasmidborne virulence genes may readily be exchanged by several bacterial genera; some may be transferred along with antibiotic resistance on resistance transfer factors (204).…”

Section: Microbial Ecology Of the Human Gastrointestinal Tractmentioning

confidence: 99%

Contemporary issues: diseases with a food vector

1988

View full text Add to dashboard Cite

Foodborne disease has become a contemporary issue. Several large, well-publicized outbreaks of foodborne disease have heightened public awareness that harmful microorganisms may be present in food and that chronic as well as acute disease may be caused by foodborne microbes. The field of food microbiology has likewise experienced a resurgence of interest. New tools, such as recombinant deoxyribonucleic acid technology and monoclonal antibody production, used to elucidate microbial virulence factors have facilitated identification of disease-causing microbes once thought to be harmless and demonstrated the complexity of individual virulence mechanisms previously considered to be well understood. Foodborne pathogens are also causing disease via some surprising food vectors, such as chopped, bottled garlic and sauteed onions. In addition to acute gastrointestinal disturbances, certain microorganisms may, through complex interactions with the human immune response, cause chronic diseases that affect several major organ systems. These microbes are serving as models in studies of molecular mimicry and genetic interrelatedness of procaryotes and eucaryotes. Other recently recognized attributes of foodborne microorganisms, such as the heat shock phenomenon and the possible nonculturability of some bacteria, may affect their ability to cause disease in humans. Because foodborne disease is a major cause of morbidity and mortality, the study of these diseases and their causative microorganisms presents a unique challenge to many professionals in the subdisciplines of microbiology, epidemiology, and clinical medicine.

show abstract

“…Acute diarrhea is a significant worldwide health problem, especially for residents of, and travellers to, less developed nations. ETEC is a major cause of diarrheal disease in developing countries [1][2][3][4]. There is general agreement that maximal immunoprotection against ETEC is elicited by natural clinical infection, in which there is a high degree of intimate contact between bacterial anti-0920-8534/88/$03.50 ~ 1988 Federation of European Microbiological Societies gens, including virulence factors, and the intestinal immune system [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Immunoprotective oral whole cell vaccine for enterotoxigenic Escherichia coli diarrhea prepared by in situ destruction of chromosomal and plasmid DNA with colicin E2

Evans

1988

FEMS Microbiology Letters

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli and Other Gram-Negative Bacteria of Infantile Diarrhea: Surface Antigens, Hemagglutinins, Colonization Factor Antigen, and Loss of Enterotoxigenicity

Cited by 63 publications

References 45 publications

Interspecific Plasmid Transfer and Modification of Heat-Stable Enterotoxin Expression by Klebsiella pneumoniae from Infants with Diarrhea

Interspecific Plasmid Transfer and Modification of Heat-Stable Enterotoxin Expression by Klebsiella pneumoniae from Infants with Diarrhea

Contemporary issues: diseases with a food vector

Immunoprotective oral whole cell vaccine for enterotoxigenic Escherichia coli diarrhea prepared by in situ destruction of chromosomal and plasmid DNA with colicin E2

Contact Info

Product

Resources

About