Enteropathogenic
 Escherichia coli
 Strains Carrying Genes Encoding the PER-2 and TEM-116 Extended-Spectrum β-Lactamases Isolated from Children with Diarrhea in Uruguay

Vignoli, Rafael; Varela, Gustavo; Mota, María Inés; Cordeiro, Nicolás F.; Power, Pablo; Ingold, Elizabet; Gadea, Pilar; Sirok, Alfredo; Schelotto, Felipé; Ayala, Juan A.; Gutkind, Gabriel

doi:10.1128/jcm.43.6.2940-2943.2005

Cited by 55 publications

(43 citation statements)

References 19 publications

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“…We did not detect bla OXA , bla CTX-M-2 , or bla CTX-M-8 in any sample ( Table 1). The prevalence of b-lactamase genes in EPEC isolated in this study differed from that isolated in the study by Vignoli et al (27), who reported the detection of bla PER-2 and bla TEM-116 b-lactamase genes in all 13 ampicillin-resistant EPEC strains isolated from children with diarrhea. Poeta et al (28) demonstrated bla TEM gene mediated ampicillin-resistance in EPEC.…”

Section: Discussioncontrasting

confidence: 99%

Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic Escherichia coli Isolated from Children with Diarrhea in Southern China

et al. 2015

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SUMMARY:The main objective of this study was to investigate the epidemiology, drug resistance and b-lactamase genotype distribution of enteropathogenic Escherichia coli (EPEC) isolated from pediatric patients with diarrhea in southern China. The prevalence of EPEC in children with diarrhea was 3.53z. The commonest serotypes were O55:K59 and O126:K71, and the typical EPEC were more prevalent than atypical EPEC (51 vs 7). Isolates from this region were most commonly found to be resistant to ampicillin and cotrimoxazole, followed by chloramphenicol, ceftriaxone, and ceftazidime. More than 96z of the strains were susceptible to cefoperazone/sulbactam and imipenem. The most common b-lactamase genotypes identified in 58 strains were bla CTX-M-1 (60.3z), bla TEM (56.9z), bla CTX-M-9 (27.6z), and bla SHV (15.5z). Among 58 isolates, 22 strains were found to harbor one b-lactamase gene, and the proportions of resistance to ampicillin, cotrimoxazole, chloramphenicol, ceftriaxone, and ceftazidime, were 81.8z, 63.6z, 40.9z, 18.2z, and 9.1z, respectively. A further 30 strains carrying multiple blactamase genes had increased resistance to the above antimicrobial agents (100z, 83.3z, 70.0z, 60.0z, and 30.0z, respectively). In contrast, antibiotic resistance in the last 6 strains without a detectable b-lactamase gene was substantially reduced. Drug resistance may be associated with the b-lactamase gene number, with a greater the number of b-lactamase genes resulting in higher antibiotic resistance.

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Section: Discussioncontrasting

confidence: 99%

Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic Escherichia coli Isolated from Children with Diarrhea in Southern China

et al. 2015

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“…EPEC strains encoding for ESBLs isolated from children have widely been reported in various parts of the world [56][57][58][59]. Nevertheless, no ESBL-producing EPEC strain was detected in this study.…”

Section: Discussionmentioning

confidence: 53%

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Ifeanyi

Ikeneche

Bassey

et al. 2017

J Infect Dev Ctries

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Introduction: Enteropathogenic Escherichia coli (EPEC) causes infectious diarrhea among children in developing countries. However, in Nigeria, due to limited laboratory resources, the genetic diversity of its virulence factors, which include intimin subtypes, remains undefined. Methodology: EPEC isolates from diarrheic children 60 months of age and younger in Abuja, Nigeria, were analyzed. Polymerase chain reaction (PCR) for EPEC virulence gene, Hep-2 cell adherence, and serotyping were performed. EPEC strains were further subtyped by PCR for the identification of intimin subtype genes α (alpha), β (beta), γ1 (gamma-1), and έ (epsilon). Antibiotic resistance and extended-spectrum beta-lactamase (ESBL) production was determined by Clinical and Laboratory Standards Institute guidelines. Results: Overall, 18 (4.5%) out of 400 children with acute diarrhea had EPEC infection. Typical EPEC (tEPEC) strains were detected in 14 (3.5%), whereas 4 (1.1%) were atypical EPEC (aEPEC). A total of 15 (83.3%) of the EPEC isolated belonged to β intimin subtype gene, while the remaining 3 EPEC isolates possessed the intimin έ subtype. No α and γ intimin subtypes were detected. Traditional EPEC serotypes O114:H14 were detected only in tEPEC strains. Marked resistance to β-lactam agents were observed but no ESBL-producing tEPEC or aEPEC was detected. Conclusions: This is the first report of intimin subtype genes in Abuja, Nigeria. EPEC isolates of diverse serotypes resistant to β-lactam antimicrobial agents were observed. These data will be useful in facilitating the characterization of intimin variants of EPEC and some Shiga toxin-producing E. coli (STEC) in humans and other animal species.

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“…Encoding genes for the SHV family were detected in 10% of the isolates. Most enzymes from this group are ESBL, except for SHV-1 and SHV-11 [25][26]. However, the enzymes from this group cannot be confirmed to be ESBLencoding genes, mostly in strains presenting more than one bla gene.…”

Section: Discussionmentioning

confidence: 87%

“…Therefore, it was not possible to determine if they were responsible for the ESBL phenotype or not; they could be non-ESBL encoding genes associated with another gene expressing the extended-spectrum phenotype [27]. On the other hand, co-production of different ESBLs by a unique strain has been a common finding in several studies [18,[25][26]28], so the carriage of two or three ESBL-encoding genes by the TEM-producing strains of this study could also have occurred.…”

Section: Discussionmentioning

confidence: 87%

Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria

Nedjai

Barguigua

Djahmi

et al. 2013

J Infect Dev Ctries

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Introduction: Expended spectrum β-lactamase (ESBL)-producing Enterobacter cloacae is an important nosocomial pathogen. In this study, the prevalence and the molecular epidemiology of ESBL producing E. cloacae strains isolated from various hospitals in Annaba, Algeria were investigated. Methodology: The study involved 63 isolates of E. cloacae obtained during 2009 at the four hospitals in Annaba. The detection of ESBL was performed using the double-disk synergy test and the combined disk test. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. The presence of bla CTX-M , bla SHV , bla TEM , and bla DHA β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis (PFGE) analysis. The clinical and microbiological data were entered into the EpiI Info database. Results: Thirty isolates (47.6%) had an ESBL phenotype. Bla CTX-M group1 (76%); bla TEM (70%) were the most prevalent, followed by bla DHA (16.6%) and bla SHV (10%). Eighteen strains expressed at least two bla genes. MICs revealed a high level of resistance to cefotaxime, ceftazidime, and cefepime. PFGE revealed an epidemic clonal dissemination of these isolates. Various risk factors associated with the occurrence of ESBL-producing E. cloacae were detected. Conclusions: A higher frequency of ESBL-producing isolates and a diversity of β-lactamases were detected among ESBL-producing E. cloacae; these resulted from an epidemic clonal dissemination and high transference of ESBL genes between bacteria in hospital settings. Strict measures will be required to control the further spread of these pathogens in hospital settings.

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Enteropathogenic Escherichia coli Strains Carrying Genes Encoding the PER-2 and TEM-116 Extended-Spectrum β-Lactamases Isolated from Children with Diarrhea in Uruguay

Cited by 55 publications

References 19 publications

Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China

Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria

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Enteropathogenic Escherichia coli Strains Carrying Genes Encoding the PER-2 and TEM-116 Extended-Spectrum β-Lactamases Isolated from Children with Diarrhea in Uruguay

Cited by 55 publications

References 19 publications

Epidemiology, Antimicrobial Resistance and &beta;-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China

Epidemiology, Antimicrobial Resistance and &beta;-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria

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Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China

Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic <i>Escherichia coli</i> Isolated from Children with Diarrhea in Southern China