Enteropancreatic Circulation of Digestive Enzymes

C, Leibow; Rothman, Stephen

doi:10.1126/science.1154022

Cited by 97 publications

(37 citation statements)

References 18 publications

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“…If active trypsin had been present, one would have expected to find it bound to its major serum protease inhibitors, a1-antitrypsin and a%-macroglobulin (23). This finding also contradicts a possible absorption of pancreatic enzymes from the intestine in smokers (24).…”

Section: Discussioncontrasting

confidence: 46%

“…Immunoreactive cationic trypsinogen. The mean concentration of cationic trypsinogen in the test serum was 37 ,ugliter before the secretin injection with a corresponding value in the control group of 24 ,ug/liter (P < 0.01). 15 and 30 min after the secretin stimulation, the mean values in serum were 176 and 174 ,ug/liter, respectively (P < 0.001) (Fig.…”

Section: Resultsmentioning

confidence: 95%

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Elevated serum levels of pancreatic secretory proteins in cigarette smokers after secretin stimulation.

Balldin¹,

Borgström²,

Eddeland³

et al. 1980

J. Clin. Invest.

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A B S T R A C T The secretory pancreatic proteins in serum were analyzed in a group of cigarette smokers and a control group of nonsmokers before and after intravenous secretin stimulation. None of these persons had any signs of pancreatic disease. In the control group, serum total amylase activity, pancreatic isoamylase, cationic trypsinogen, and pancreatic secretory trypsin inhibitor concentrations varied within the normal range before and after secretin injection. In contrast, the concentrations of these pancreatic proteins in all the cigarette smokers elevated from normal to abnormally high serum concentrations after secretin stimulation. The results indicate a probable toxic effect of cigarette smoking on the exocrine pancreas.

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Section: Discussioncontrasting

confidence: 46%

Section: Resultsmentioning

confidence: 95%

Elevated serum levels of pancreatic secretory proteins in cigarette smokers after secretin stimulation.

Balldin¹,

Borgström²,

Eddeland³

et al. 1980

J. Clin. Invest.

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“…Due to the alteration of the intestinal permeability, endotoxins enter the general circulation [33], but the entry of digestive enzymes, recognised as normal (in humans) to some extent [8,26] can also be increased. The implication of the pancreas or pancreatic enzymes in acute abdominal disease in the horse has not, to date, been well documented.…”

Section: Introductionmentioning

confidence: 99%

Equine trypsin: purification and development of a radio-immunoassay

et al. 2003

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-Shock is accompanied by generalised splanchnic hypoperfusion, and splanchnic organs like the pancreas can be damaged, as shown in animal experimental models and in humans, by the presence of high plasma concentrations of trypsin and other pancreatic enzymes. In order to design a radioimmunoassay technique (RIA) for the measurement of equine trypsin-like immunoreactivity (TLI) in biological fluids, trypsin was purified (with purity 96 %) from the equine pancreas by extraction in an acid medium, ammonium sulfate precipitations, gel filtration chromatography and, after activation of trypsinogen into trypsin, affinity chromatography. Gel polyacrylamide electrophoresis showed a monomeric enzyme with a molecular weight of 27 kDa. The purified equine trypsin served for the immunisation of rabbits in order to obtain a specific antiserum, and the labelled antigen was prepared by iodination of equine trypsin with 125 I. The RIA was based on the binding of the antigen to the antibody followed by the separation of the antigen-antibody complex by immunoprecipitation in the presence of sheep anti-rabbit gammaglobulins and the assay of the radioactivity in the precipitate. The RIA showed good sensitivity, specificity, precision, accuracy and reproducibility. The reference mean value of TLI in the plasma of healthy horses (n = 20) was 30.01 ± 6.84 ng/mL (upper confidence limit 50.52 ng/mL; p < 0.01). Three horses with non strangulating intestinal obstruction without shock showed TLI values within normal limits whereas 5 of 7 horses with strangulation obstruction showed TLI levels above the upper confidence limit. Further studies using the RIA and the enzymatic assay should be performed in order to confirm the role of the pancreas in equine intestinal obstruction. equine trypsin / purification / radio-immunoassay / reference range / intestinal obstruction ³

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“…This phenomenon is not uncommon in physiologyf and recent pertinent literature indicates uptake of bovine serum albumin by the lateral line system of trout (1), of the digestive enzymes (chymotrypsin, amylase, and papain) or albumin from the intestine (7,22; Murachi, Abstr. 10th Int.…”

Section: Downloaded Frommentioning

confidence: 99%

Mechanism of Accumulation of the Antitumor Protein Antibiotic Neocarzinostatin in Bladder Tissue: Intravenous Administration, Urinary Excretion, and Absorption into Bladder Tissue

Maeda

Sakamoto

Ogata

1977

Antimicrob Agents Chemother

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Some aspects ofthe absorption, distribution, and excretion of neocarzinostatin (NCS), a proteinous antitumor antibiotic, were studied in rabbits. NCS was given intravenously (i.v.) via the auricular vein, or [14I]NCS was instilled directly into the cavity of the bladder by tubing. In both groups, ureterostomy was performed, so that the drug excreted in the urine did not pass through the bladder. The results showed extremely rapid renal clearance; namely, twothirds of the total recovered was excreted in the first 5 min. It was also shown that drug infused into the bladder cavity could be recovered in urine from the ureterostomized ureter. Also, the level of biological activity of NCS in bladder tissues after i.v. administration is significantly lower when ureterostomy is performed. Thus, evidence is presented for the absorption of NCS into bladder tissue from the lumen of the bladder. The high levels of NCS in bladder tissue are due to this effect as well as to accumulation via the iliac artery. These data should encourage further trials of NCS in bladder cancer. A study of urine containing NCS derived from i.v. administration showed an increase in antibacterial activity upon incubation, followed by a decrease. These effects are probably due to proteolysis, as shown by the appearance of a low-molecular-weight fragment and by the absence of such an increase in the presence of inhibitors of proteolysis.

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Enteropancreatic Circulation of Digestive Enzymes

Abstract: Intact digestive enzymes can be absorbed by the intestine and resecreted by the pancreas. The pancreas, therefore, appears to be able to recycle proteins much as the liver recycles bile salts, although the magnitude of this process remains uncertain.

Cited by 97 publications

References 18 publications

Elevated serum levels of pancreatic secretory proteins in cigarette smokers after secretin stimulation.

Elevated serum levels of pancreatic secretory proteins in cigarette smokers after secretin stimulation.

Equine trypsin: purification and development of a radio-immunoassay

Mechanism of Accumulation of the Antitumor Protein Antibiotic Neocarzinostatin in Bladder Tissue: Intravenous Administration, Urinary Excretion, and Absorption into Bladder Tissue

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