Enterohemorrhagic
            <i>Escherichia coli</i>
            Adhesins

McWilliams, Brian D.; Torres, Alfredo G.

doi:10.1128/microbiolspec.ehec-0003-2013

Cited by 109 publications

(88 citation statements)

References 193 publications

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“…Fimbriae and autotransporters are adhesion factors associated with biofilm formation in STEC (55). Fimbria-encoding genes z1538 and lpf2, the passenger domain of the autotransporter gene ehaB, and the autotransporter gene espP are present in isolates producing significantly more biofilms (Fig.…”

Section: Discussionmentioning

confidence: 99%

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

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bForming biofilms may be a survival strategy of Shiga toxin-producing Escherichia coli to enable it to persist in the environment and the food industry. Here, we evaluate and characterize the biofilm-forming ability of 39 isolates of Shiga toxin-producing Escherichia coli isolates recovered from human infection and belonging to seropathotypes A, B, or C. The presence and/or production of biofilm factors such as curli, cellulose, autotransporter, and fimbriae were investigated. The polymeric matrix of these biofilms was analyzed by confocal microscopy and by enzymatic digestion. Cell viability and matrix integrity were examined after sanitizer treatments. Isolates of the seropathotype A (O157:H7 and O157:NM), which have the highest relative incidence of human infection, had a greater ability to form biofilms than isolates of seropathotype B or C. Seropathotype A isolates were unique in their ability to produce cellulose and poly-N-acetylglucosamine. The integrity of the biofilms was dependent on proteins. Two autotransporter genes, ehaB and espP, and two fimbrial genes, z1538 and lpf2, were identified as potential genetic determinants for biofilm formation. Interestingly, the ability of several isolates from seropathotype A to form biofilms was associated with their ability to agglutinate yeast in a mannose-independent manner. We consider this an unidentified biofilmassociated factor produced by those isolates. Treatment with sanitizers reduced the viability of Shiga toxin-producing Escherichia coli but did not completely remove the biofilm matrix. Overall, our data indicate that biofilm formation could contribute to the persistence of Shiga toxin-producing Escherichia coli and specifically seropathotype A isolates in the environment. Shiga toxin-producing Escherichia coli (STEC) strains, including enterohemorrhagic E. coli (EHEC), are food-borne and waterborne human enteric pathogens responsible for infections. They are associated with important public health concerns in developed countries. Symptoms associated with STEC infections range from abdominal cramps and bloody diarrhea to postinfectious complications, such as hemolytic-uremic syndrome (HUS). HUS, a life-threatening complication of STEC infections, is a consequence of Shiga toxin production. According to the Public Health Agency of Canada (PHAC), more than 30,000 cases of STEC infections occur each year in Canada (1). Environmentally, the main reservoir for STEC is cattle. Transmission of STEC to humans typically is associated with the consumption of contaminated food such as undercooked beef, fresh produce, unpasteurized milk, or contaminated drinking water (2), but these pathogens also can be transmitted from person to person or via direct contact with animals or their feces.The 2003 Karmali seropathotype model classifies STEC into seropathotypes based on their reported incidence in human disease, outbreaks, and/or association with the development of severe symptoms in humans (3). Serotypes frequently responsible for hemorrhagic colitis (HC) and HUS, ...

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Section: Discussionmentioning

confidence: 99%

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

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“…Of these, 5 have been frequently identified worldwide and are most pathogenic for humans together with O157:H7: O26:H11, O103:H2, O111:H-, O121:H19, and O145:H-. In addition to the phage-located stx1 and stx2 genes, those AE-STEC can harbour several other genes coding for virulence-associated factors: a set of genes on a pathogenicity island named the Locus of Enterocyte Effacement (LEE) code for a type three secretion system (T3SS), T3-secreted effectors and the outer membrane intimin adhesin (the eae gene); different chromosomal non LEE-located genes code for other T3-secreted effectors and for other adhesins or toxins; and still other genes located on a plasmid (pO157 plasmid) code for several virulence markers, including one enterohaemolysin (Ehly) (Mainil and Daube, 2005;Bugarel et al, 2010;Beutin and Fach, 2015;McWilliams and Torres, 2015;Stevens and Frankel, 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC

Fakih

Thiry

Duprez

et al. 2017

Veterinary Microbiology

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A B S T R A C TEscherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www.genomicepidemiology.org). Of 233 isolates, 206 were triplex PCR-positive: 119 AE-STEC (58%), 78 EPEC (38%) and 9 STEC (4%); and the stx1+eae+ AE-STEC (49.5%) were the most frequent. Of them, 120 isolates (84% of AE-STEC, 23% of EPEC, 22% of STEC) tested positive with one O serogroup PCR: 57 for O26 (47.5%), 36 for O111 (30%), 10 for O103 (8%) and 8 for O5 (7%) serogroups. The analysis of the draft sequences of 15 O5 AE-STEC could not identify any difference correlated to the host. As a conclusion, (i) the AE-STEC associated with diarrhoea in young calves still belong to the same serogroups as previously (O5, O26, O111) but the O103 serogroup may be emerging, (ii) the O5 AE-STEC from calves and humans are genetically similar.2016 Published by Elsevier B.V.

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“…Las adhesinas son un grupo de proteínas involucradas en la unión de E. coli tanto a superficies abióticas (plástico y acero), como a superficies vivas (McWilliams y Torres, 2014).…”

Section: Adhesión Bacteriana Y Sobrevivencia En Las Plantasunclassified

“…Adhesins are a group of proteins involved in the attachment of E. coli to abiotic surfaces such as plastic or steel, and also the colonization of biological surfaces (McWilliams and Torres, 2014).…”

Section: Adhesión Bacteriana Y Sobrevivencia En Las Plantasmentioning

confidence: 99%

Interacciones entre Escherichia coli O157:H7 y Plantas Comestibles. ¿Se han Desarrollado Mecanismos de Internalización Bacteriana?

Torres-Aguilar

Manjarrez-DomÃnguez

Acosta-MuÃ±iz

et al. 2015

RMF

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Abstract. Despite efforts to prevent microbial contamination, the occurrence of human pathogens in fresh fruit and vegetable is quite common. It is now known that E. coli O157:H7 can inhabit food plants passively. Here, we review the literature on phylloplane adhesion, survival and internalization of this bacterium, as well as some of the interaction mechanisms between it and the food plant, and other Resumen. La presencia de bacterias patógenas en frutas y hortalizas es muy común a pesar de los grandes esfuerzos realizados para prevenir la contaminación microbiologica. En la actualidad, se sabe (o se conoce) que E. coli O157:H7 puede colonizar de forma pasiva plantas comestibles. Este trabajo presenta una revisión de literatura sobre E. coli y su adhesión al filoplano, supervivencia e internalización, así como mecanismos de interacción bacteria -planta. Una vez, que este patógeno, se adhiere a la superficie de la planta, puede introducirse al tejido, para después movilizarse y multiplicarse. Esta revisión ofrece una visión general de los mecanismos de interacción entre el tejido vegetal y E. coli O157:H7. Se considera que este patógeno ha mejorado su competencia ecológica en plantas comestibles, sin perder su virulencia para el ser hu- Interactions between

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Enterohemorrhagic Escherichia coli Adhesins

Cited by 109 publications

References 193 publications

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC

Interacciones entre Escherichia coli O157:H7 y Plantas Comestibles. ¿Se han Desarrollado Mecanismos de Internalización Bacteriana?

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