Enterohemorrhagic E. coli effector NleL disrupts host NF-κB signaling by targeting multiple host proteins

Sheng, Xiangpeng; You, Qing; Zhu, Hongnian; Li, Qingrun; Gao, Hong; Wang, Haifeng; You, Chunping; Meng, Qingchang; Nie, Yingjie; Zhang, Xiangyan; Hu, Ronggui

doi:10.1093/jmcb/mjaa003

Cited by 10 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lncRNA with the highest expression level was TCONS_00211357, which was predicted to target several NF-κB pathway-related genes such as TRIM38 [NF-κB activator, ( 37 )] and NFKBID [NF-κB inhibitor, ( 38 )]. Numerous studies have shown that the NF-κB pathway is involved in the host inflammatory response to E. coli ( 39 – 41 ). Our results suggest that there is a certain probability that TCONS_00211357 act as a key regulator of NF-κB pathway in immune response to E. coli F17 infection.…”

Section: Discussionmentioning

confidence: 99%

Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17

Chen

Zhang

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

It has long been recognized that enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for vomiting and diarrhea. E. coli F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying E. coli F17 infection has not been fully understood. In the present study, RNA sequencing was conducted to explore the expression profiles of mRNAs and long non-coding RNAs (lncRNAs) in the jejunum of lambs who were identified as resistant or sensitive to E. coli F17 that was obtained in a challenge experiment. A total of 772 differentially expressed (DE) mRNAs and 190 DE lncRNAs were detected between the E. coli F17—resistance and E. coli F17-sensitive lambs (i.e., TFF2, LOC105606142, OLFM4, LYPD8, REG4, APOA4, TCONS_00223467, and TCONS_00241897). Then, a two-step machine learning approach (RX) combination Random Forest and Extreme Gradient Boosting were performed, which identified 16 mRNAs and 17 lncRNAs as potential biomarkers, within which PPP2R3A and TCONS_00182693 were prioritized as key biomarkers involved in E. coli F17 infection. Furthermore, functional enrichment analysis showed that peroxisome proliferator-activated receptor (PPAR) pathway was significantly enriched in response to E. coli F17 infection. Our finding will help to improve the knowledge of the mechanisms underlying E. coli F17 infection and may provide novel targets for future treatment of E. coli F17 infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17

Chen

Zhang

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…NleL has been studied for its contributions to the attaching and effacing mechanism of EHEC, but conflicting results make the exact function of NleL in this pathway unclear (Piscatelli et al , 2011; Sheng et al , 2017). Additionally, another recent study provided evidence that NleL may be targeting components of the NF‐κB immune signaling pathway (Sheng et al , 2020).…”

Section: Ubiquitin Ligation By Cysteine‐dependent E3 Ligasesmentioning

confidence: 99%

Ubiquitin‐targeted bacterial effectors: rule breakers of the ubiquitin system

2023

View full text Add to dashboard Cite

Regulation through post‐translational ubiquitin signaling underlies a large portion of eukaryotic biology. This has not gone unnoticed by invading pathogens, many of which have evolved mechanisms to manipulate or subvert the host ubiquitin system. Bacteria are particularly adept at this and rely heavily upon ubiquitin‐targeted virulence factors for invasion and replication. Despite lacking a conventional ubiquitin system of their own, many bacterial ubiquitin regulators loosely follow the structural and mechanistic rules established by eukaryotic ubiquitin machinery. Others completely break these rules and have evolved novel structural folds, exhibit distinct mechanisms of regulation, or catalyze foreign ubiquitin modifications. Studying these interactions can not only reveal important aspects of bacterial pathogenesis but also shed light on unexplored areas of ubiquitin signaling and regulation. In this review, we discuss the methods by which bacteria manipulate host ubiquitin and highlight aspects that follow or break the rules of ubiquitination.

show abstract

“… 38 A recent work revealed that NleL disrupted the host NF-κB signaling by targeting several components of the NF-κB pathway, including TRAF2, TRAF5, TRAF6, IKKα, and IKKβ. 39 …”

Section: Ois Encoding Effectorsmentioning

confidence: 99%