Enterocolitis in piglets caused by Cryptosporidium sp. purified from calf faeces

Tzipori, Saul; Smith, Mickey C.; Makin, T.; Halpin, C. G.

doi:10.1016/0304-4017(82)90033-4

Cited by 50 publications

(37 citation statements)

References 9 publications

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“…The relationship of infections with clinical symptoms, primarily diarrhoea, varies in pigs. Both C. parvum (calf origin) and C. hominis (human origin) have been reported to cause clinical cryptosporidiosis in pigs (diarrhoea) (Moon and Bemrick 1981;Moon et al 1982;Tzipori et al 1981Tzipori et al , 1982Argenzio et al 1990;Vítovec and Koudela 1992;Ebeid et al 2003), but no diarrhoeal outbreaks caused in these species have ever been reported in naturally infected pigs. Also, Xiao et al (1994) and Quílez et al (1996) observed severe and intense infections with Cryptosporidium spp.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Němejc¹,

Sak²,

Květoňová³

et al. 2012

Parasitol Res

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From 2009 to 2011, the occurrence of Cryptosporidium spp. was investigated on 22 farms in the Czech Republic. A total of 1,620 individual faecal samples of pigs of all age categories (preweaned, starters, pre-growers, growers, and sows) were evaluated for presence of Cryptosporidium spp. by standard microscopy and molecular tools. Genotyping was done through PCR amplification and characterization of the SSU rRNA (species-specific protocols) and GP60 loci. Cryptosporidium spp. was found on 16 of 22 farms with a range 0.9-71.4 %. Overall, 194 (12 %) specimens were positive by microscopy and 353 (21.8 %) by PCR. While RFLP and direct sequencing of the PCR-amplified products showed presence of Cryptosporidium suis (142), kvac@centrum.cz. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptCryptosporidium scrofarum (195), Cryptosporidium muris (3) and 13 samples had mixed infections with C. suis and C. scrofarum, species-specific molecular tools identified C. suis (224), C. scrofarum (208), Cryptosporidium parvum subtype IIa A16G1R1b (1), and C. muris (3). In addition, a total of 82 pigs had concurrent infections with C. suis and C. scrofarum. The analysis by age showed that C. suis was primarily detected among pre-weaned, whereas C. scrofarum was mostly detected among starters, especially those weaned at a younger age. Moreover, C. scrofarum never has been detected in animals younger than 6 weeks of age. Also, piglets weaned at 3 weeks of age were twice more likely to be infected with C. scrofarum than piglets weaned at an older age. Pigs raised on straw bedding were more likely to have Cryptosporidium than pigs raised on slats/slurry systems. The infections with different species were not associated with loose faeces or intensity of oocyst shedding, even when comparing different age groups.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Němejc¹,

Sak²,

Květoňová³

et al. 2012

Parasitol Res

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“…Pig cryptosporidiosis can be caused by three distinct species/genotypes of Cryptosporidium: Cryptosporidium parvum, Cryptosporidium suis, or Cryptosporidium pig genotype II. Although natural infections with C. parvum on the pigs farms have been described only rarely (Morgan et al 1999;Zintl et al 2007), the infectivity and pathogenicity of C. parvum have been experimentally confirmed on conventional and gnotobiotic piglets (Moon and Bemrick 1981;Tzipori et al 1982;Vítovec and Koudela 1992). The other two species/genotypes, C. suis and Cryptosporidium pig genotype II, are frequently found in pigs.…”

Section: Introductionmentioning

confidence: 94%

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

et al. 2008

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A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

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“…Neither oocysts nor positive titers of serum IgG antibodies were detected in suckling piglets, although we cannot rule out the possibility that these animals received antibodies via passive transfer, since all of them were more than 15 days old and the maternal antibody levels drop before that time (Salmon et al 1990). Indeed, the high percentage of sows found to be seropositive (53.3%) suggests that suckling piglets received a good dose of maternally derived lactogenic immunity, which may protect them up to the age of 12-15 days, after which they usually become inherently resistant (Tzipori et al 1982), a condition stated by Tzipori (1985) to explain the low prevalence of natural infection in suckling piglets.…”

Section: Ifa Titermentioning

confidence: 99%

Comparison of oocyst shedding and the serum immune response to Cryptosporidium parvum in cattle and pigs

et al. 1996

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A comparison was made between oocyst shedding and the presence of specific serum IgG antibodies to Cryptosporidium parvum in 108 bovines and 90 pigs. Oocysts were detected by a commercial immunofluorescence assay in feces from 26.8% of bovines and 34.4% of pigs, whereas positive titers as determined by an indirect fluorescent antibody method were found in sera from 12.9% and 48.9% of the respective animals. Infection was significantly most frequent in suckling calves (82.7%) and weaned piglets (87.5%). By contrast, the numbers of seropositives were highest in weaned calves (17.1%) and fattening pigs (76.6%). The results of coprological and serological analysis corresponded in 65.7% of bovines and 56.7% of pigs. When used to diagnose the shedding of cryptosporidial oocysts, the detection of specific IgG antibodies had a sensitivity ranging from 10.3% (cattle) to 58.1% (pigs) and a specificity of 86.1% (cattle) and 55.9% (pigs).

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Enterocolitis in piglets caused by Cryptosporidium sp. purified from calf faeces

Cited by 50 publications

References 9 publications

Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Comparison of oocyst shedding and the serum immune response to Cryptosporidium parvum in cattle and pigs

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