Enterococcus villorum sp. nov., an enteroadherent bacterium associated with diarrhoea in piglets.

Abstract

The taxonomic positions of five enteroadherent bacterial pig isolates, showing phenotypic characteristics most similar to those of Enterococcus durans and Enterococcus hirae, were investigated in a polyphasic study that included 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base-ratio determinations, whole-cell protein fingerprinting, D11344-primed PCR typing and an extensive examination of phenotypic properties. The results demonstrated that the organisms represent a new species in the Enterococcus faecium species group, for which the name Enterococcus villorum sp. nov. is proposed. The type strain is LMG 12287 T (l CCM 4887 T ).

Lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons

“…For determination of the DNA base composition, DNA was enzymically degraded into nucleosides and then separated by HPLC as described previously (Vancanneyt et al, 2001).…”

“…High-molecular-mass native DNA was prepared as described above for determination of the DNA base composition. DNA-DNA hybridizations were performed as described by Vancanneyt et al (2001) by using a microplate method and fluorescence measurements for calculation of the binding values. Hybridizations were performed at 41˚C.…”

Emended description of Streptococcus ferus isolated from pigs and rats

“…Before addition of GES reagent, proteinase K (200 mg ml 21 ; Merck) was added and the mixture was incubated for 15 min. DNA was enzymically degraded into nucleosides and separated by HPLC as described previously (Vancanneyt et al, 2001). The G+C contents of strains LMG 19829 and LMG 19830 were respectively 43?0 and 42?7 mol%.…”

Fluorescent in situ hybridization with specific DNA probes offers adequate detection of Enterococcus faecalis and Enterococcus faecium in clinical samples

“…A positive result from using this probe on clinical samples will almost always indicate detection of E. faecium, because E. hirae and E. mundtii have been isolated only rarely from human sources and then usually as colonizers of humans and not from blood cultures or other clinically important materials (Facklam & Collins, 1989;Gilad et al, 1998;Kaufhold & Ferrieri, 1991;Ruoff et al, 1990). E. saccharolyticus and E. villorum have not been isolated from human sources (Facklam et al, 1999;Vancanneyt et al, 2001). When using FISH with the Enfm93 probe on very complex mixed bacterial populations, e.g.…”