Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment

Abstract

A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629 T 5LMG 26676 T 5CCUG 48799 T ), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630 T 5LMG 26678 T 5CCUG 61593 T ), inhabiting water, insects (mosquitoes) and plants, are proposed.

Biotic Interactions and Sunlight Affect Persistence of Fecal Indicator Bacteria and Microbial Source Tracking Genetic Markers in the Upper Mississippi River

“…Recreational water quality is typically assessed using culturebased enumeration of fecal indicator bacteria (FIB) (e.g., Escherichia coli and enterococci) worldwide and, more recently, quantitative real-time PCR (qPCR) in the United States (4), neither of which provides information about the pollution source, since these organisms are commensal inhabitants of the gastrointestinal tracts of mammals, birds, and various insects (5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Microbial source tracking (MST) has emerged in response to the increasing need to identify and manage sources of fecal pollution, and it aims to remedy the situation through identification of contributing fecal sources by targeting genetic markers thought to be closely associated with particular animal hosts (15).…”

Vancomycin resistant Enterococcus colonization: Is isolation the only precaution?

“…The genus Enterococcus belongs to the Enterococcaceae family along with the Atopobacter, Catellicoccus, Melissococcus, Pilibacter, Tetragenococcus and Vagococcus genera [7]. The Enterococcus genus currently consists of 35 recognised species, with eight more species (Enterococcus lactis, Enterococcus plantarum, Enterococcus quebecensis, Enterococcus rivorum, Enterococcus rotai, Enterococcus ureasiticus, Enterococcus ureilyticus, and Enterococcus viikiensis) likely to be added [8][9][10][11][12][13].…”

Innovative Analysis of the Sequenced Patterns of Vancomycin-ResistantEnterococciStrains to Determine Clonal Transmission in a Hospital Setting

“…Repetitive sequence-based polymerase chain reaction (REP-PCR) strain typing has been used in other studies to discriminate isolates as genetically identical. [7][8][9] To perform REP-PCR using a UltraClean Microbial DNA Isolation Kit (MO-BIO Laboratories, Solana Beach, CA), genomic DNA was extracted from 1 µL of organism. A working concentration of 25-50 ng/mL DNA was verified using a NanoDropTM ND-1000 spectrophotometer.…”