Enterococcus faecium; a Suitable Probiotic Candidate for Modulation of Immune Responses Against Pathogens

Khalkhali, Soodabeh; Mojgani, Naheed

doi:10.15171/ijbsm.2017.15

Cited by 10 publications

(10 citation statements)

References 29 publications

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“…More specifically, activation of human and murine DCs by E. faecalis was demonstrated [95]. However, few data are available about the effects exerted on the immune system by E. faecium [96]. Here, we tested both living and heat-killed cells (HK) since the experiment with the living cells damaged the DCs cultures.…”

Section: Possible Interaction With the Hostmentioning

confidence: 99%

“…When specifically considering their biological functions, both CD80 and CD83 relate to T lymphocytes activation. In the literature, experiments describing the effects of probiotic E. faecium strains on the host immune system only refer to increased neutrophil phagocytosis and humoral immunity [96]. Therefore, no comparison with known data is possible.…”

Section: Possible Interaction With the Hostmentioning

confidence: 99%

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Serotonin Exposure Improves Stress Resistance, Aggregation, and Biofilm Formation in the Probiotic Enterococcus faecium NCIMB10415

Scardaci

Manfredi

Barberis

et al. 2021

Microbiology Research

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The role of the microbiota–gut–brain axis in maintaining a healthy status is well recognized. In this bidirectional flux, the influence of host hormones on gut bacteria is crucial. However, data on commensal/probiotics are scarce since most reports analyzed the effects of human bioactive compounds on opportunistic strains, highlighting the risk of increased pathogenicity under stimulation. The present investigation examined the modifications induced by 5HT, a tryptophan-derived molecule abundant in the intestine, on the probiotic Enterococcus faecium NCIMB10415. Specific phenotypic modifications concerning the probiotic potential and possible effects of treated bacteria on dendritic cells were explored together with the comparative soluble proteome evaluation. Increased resistance to bile salts and ampicillin in 5HT-stimulated conditions relate with overexpression of specific proteins (among which Zn-beta-lactamases, a Zn-transport protein and a protein involved in fatty acid incorporation into the membrane). Better auto-aggregating properties and biofilm-forming aptitude are consistent with enhanced QS peptide transport. Concerning interaction with the host, E. faecium NCIMB10415 enhanced dendritic cell maturation, but no significant differences were observed between 5HT-treated and untreated bacteria; meanwhile, after 5HT exposure, some moonlight proteins possibly involved in tissue adhesion were found in higher abundance. Finally, the finding in stimulated conditions of a higher abundance of VicR, a protein involved in two-component signal transduction system (VicK/R), suggests the existence of a possible surface receptor (VicK) for 5HT sensing in the strain studied. These overall data indicate that E. faecium NCIMB10415 modifies its physiology in response to 5HT by improving bacterial interactions and resistance to stressors.

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Section: Possible Interaction With the Hostmentioning

confidence: 99%

Section: Possible Interaction With the Hostmentioning

confidence: 99%

Serotonin Exposure Improves Stress Resistance, Aggregation, and Biofilm Formation in the Probiotic Enterococcus faecium NCIMB10415

Scardaci

Manfredi

Barberis

et al. 2021

Microbiology Research

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“…All types of microorganisms that developed have positive characteristics. Enterococcus faecium has probiotic properties because of its ability to modulate the immune system of the host and thus improve responses against pathogens (Khalkhali and Mojgani 2017). Streptomycetes sp.…”

Section: Difference In the Effect Of The Three Compostsmentioning

confidence: 99%

Effect of three types of liquid compost combined with Avicennia marina leaves on growth and survival of tiger prawns (Penaeus monodon)

et al. 2019

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The sustainability of prawn farming in brackish water ponds is controversial because of low yields and a history of mangrove clearing. Low yields are due largely to insufficient preparation of pond bottoms. Mangrove trees are often planted on pond bunds as window dressing. This study examines the effect of three types of liquid compost from vegetable, fruit, and both vegetable and fruit in tanks to which whole or chopped Avicennia marina leaves have been added to mimic local pond conditions. In a split-plot design, 28 square tanks were each stocked with one hundred 15-day-old post-larvae tiger prawns (Penaeus monodon). Four tanks were used as controls and 24 were assigned to the treatments, 12 with whole and 12 with chopped leaves. Of the treatment tanks, 4 received liquid compost from vegetable, 4 received fruit, and 4 received mixed vegetable and fruit. Shrimp were weighed at the start, halfway point, and the end of the 50-day trial, and fed at 5% of the estimated total weight; survival was counted at the end. The survival rates of treatments and controls (65–76%) were not significantly different. Shrimp in water with vegetable compost grew significantly faster (2.7% day−1) than in both treatments with fruit (2.5% day−1), while all treatments were associated with significantly faster growth than were the controls (2.0% day−1). The lower growth rate of shrimp fed fruit compost may have been due to dinoflagellates, which are known to negatively affect shrimp. Shrimp in tanks with chopped mangrove leaves grew slightly better than shrimp in tanks with whole mangrove leaves.

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“…Enterococcus faecium showed antibacterial activity [50], probably because of having bacteriocin as possessed by Enterococci in general [51]. This bacterium had also cholesterol-lowering activity [52] and survived in acidic conditions [53].…”

Section: Analysis Of 16s Rrna Sequencesmentioning

confidence: 98%

Potential Next-Generation Probiotics Isolated from Romaine Lettuce (Lactuca sativa L. var. longifolia) Fermented Brine

Tallei¹,

Fatimawali²,

Sumual³

et al. 2021

Advances in Biological Sciences Research

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Nowadays, the search for potential probiotics from lactic acid bacteria continues to develop exclusively for pharmaceutical applications. This study aimed at evaluating the potency of lactic acid bacteria (LAB) isolated from fermented brine of romaine lettuce as next-generation probiotics. The study began with romaine lettuce fermentation in a 10% salt solution for four days at room temperature in the dark. The LAB from the fermentation liquid were grown on MRS agar supplemented with CaCo3 then purified. Purified colonies were identified using Gram-staining, catalase test, and 16S rRNA gene marker, and tested for their ability to be developed as the next-generation probiotics which included the following criteria: antibacterial activity, cholesterol assimilation, and their survival at pH3. The cholesterol-lowering ability was evaluated by incubating the bacteria in MRS agar supplemented with 0.5% taurodeoxycholic acid (TDCA). Well diffusion method was used to assess the antibacterial activity. Their ability to withstand acid environment at pH 3 was also evaluated. This study showed that all isolates (AS1, AS2, AS3, and AS4) survived at pH 3 for 2 hours and grew until the fifth day. Isolates AS2, AS3, and AS4 inhibited the growth of Staphylococcus aureus, while AS1, AS3, and AS4 inhibited the growth of Escherihia coli. All isolates had the ability to lower cholesterol. Isolate AS3 was molecularly identified as Enterococcus faecium. This isolate was chosen to be identified as it showed the best characteristics among all the isolates tested. We concluded that AS3 can be further developed as next-generation probiotic.

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Enterococcus faecium; a Suitable Probiotic Candidate for Modulation of Immune Responses Against Pathogens

Cited by 10 publications

References 29 publications

Serotonin Exposure Improves Stress Resistance, Aggregation, and Biofilm Formation in the Probiotic Enterococcus faecium NCIMB10415

Serotonin Exposure Improves Stress Resistance, Aggregation, and Biofilm Formation in the Probiotic Enterococcus faecium NCIMB10415

Effect of three types of liquid compost combined with Avicennia marina leaves on growth and survival of tiger prawns (Penaeus monodon)

Potential Next-Generation Probiotics Isolated from Romaine Lettuce (Lactuca sativa L. var. longifolia) Fermented Brine

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