Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses

Lauková, Andrea; Styková, Eva; Kubašová, Ivana; Strompfová, Viola; Gancarčíková, Soňa; Plachá, Iveta; Miltko, Renata; Bełżecki, Grzegorz; Valocký, Igor; Simonová, Monika Pogány

doi:10.1007/s12602-020-09655-6

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…Hydrolytic activities, amylolytic, cellulolytic, xylanolytic, pectinolytic and inulolytic (expressed in µmol/g/DM/min meaning in mikromol per gram of dry matter per minute) were processed as previously described Lauková et al [14]. The enzymes were extracted using the procedure of Huhtanen and Khali [15] and they were measured according to procedure by Miltko et al [16].…”

Section: Hydrolytic Activity Jejunal Morphometry Growth Performance O...mentioning

confidence: 99%

Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits

Lauková,

Chrastinová,

Focková

et al. 2024

Preprint

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Nowadays, animal breeders look for effective innovations to care animal health. Use of probiotics, postbiotics, parabiotics and the other beneficial natural substances are dominated. Mundticin-like substance EM 41/3 is thermo-stable bacteriocin (postbiotic) with a broad antimicrobial spectrum. In this study its effect in broiler rabbits was assessed. Rabbits represent food-derived animal and they are also suitable animal model. The parameters tested were analyzed by validated methods. Administration of MLS EM 41/3 produced by non-autochthonous strain Enterococcus mundtii EM 41/3 lead to significant increase of parameter immunity-phagocytic activity, while it did not influence microbiota composition. It also did not influence blood biochemistry and no oxidative stress was noted. Moreover, higher growth parameters were noted and also stimulated hydrolytic activity. Finally, no negative influence on rabbit meat quality was noted. Postbiotic MLS EM 41/3 administration in feeding strategy seems to be contributing fact for rabbit health status maintaining.

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Section: Hydrolytic Activity Jejunal Morphometry Growth Performance O...mentioning

confidence: 99%

Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits

Lauková,

Chrastinová,

Focková

et al. 2024

Preprint

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“…Bacteria commonly used as probiotics in other species (e.g., Lactobacillus , Enterococcus , Bacillus , Streptococcus and Bifidobacterium ) have been evaluated in horses [ 9 , 11 , 12 , 15 ] with underwhelming results. However, a recent study of non-authochtonous Enterococcus faecium AL41, which produces antimicrobial bacteriocin—enterocin M, showed sufficient colonization of the equine GIT, reduction in Aeromonas populations and increased activity of hydrolytic enzymes [ 66 ].…”

Section: Equine Probiotic Strainsmentioning

confidence: 99%

Gut Microbiota Manipulation in Foals—Naturopathic Diarrhea Management, or Unsubstantiated Folly?

2021

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Diarrhea in foals is a problem of significant clinical and economic consequence, and there are good reasons to believe microbiota manipulation can play an important role in its management. However, given the dynamic development of the foal microbiota and its importance in health and disease, any prophylactic or therapeutic efforts to alter its composition should be evidence based. The few clinical trials of probiotic preparations conducted in foals to date show underwhelming evidence of efficacy and a demonstrated potential to aggravate rather than mitigate diarrhea. Furthermore, recent studies have affirmed that variable but universally inadequate quality control of probiotics enables inadvertent administration of toxin-producing or otherwise pathogenic bacterial strains, as well as strains bearing transferrable antimicrobial resistance genes. Consequently, it seems advisable to approach probiotic therapy in particular with caution for the time being. While prebiotics show initial promise, an even greater scarcity of clinical trials makes it impossible to weigh the pros and cons of their use. Advancing technology will surely continue to enable more detailed and accurate mapping of the equine adult and juvenile microbiota and potentially elucidate the complexities of causation in dysbiosis and disease. In the meantime, fecal microbiota transplantation may be an attractive therapeutic shortcut, allowing practitioners to reconstruct a healthy microbiota even without fully understanding its constitution.

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“…Therefore, in this study more detaily different properties of EMo 1-1Nik strain have been studied with the aspect of its safety or its bacteriocin activity. As it is known, bacteriocins are proteinaceous substances with inhibitory effect against more or less relative bacteria (Nes et al 2014) which were successfully used in different niches such as food (Lauková and Vlaemynck 2003), environment (Lauková et al 2002) and also animals (Pogány Simonová et al 2020), horses involving (as formerly mentioned, Lauková et al 2020Lauková et al , 2022 to optimize their health status. EMo 1-1Nik looks as a promissing candidate strain.…”

Section: Introductionmentioning

confidence: 99%

Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain

et al. 2023

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Nowadays, developed more precisious identi cation techniques have allowed to identify and validate newer enterococcal species. Among those species, also the species Enterococcus moraviensis was validated, the representat of which was rstly isolated from surface waters. However, in this study, characteristic and bacteriocin potential of E. moraviensis strain EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted EMo 1-1Nik strain to the species E. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity with E. moraviensis NR113937.1 strain). The EMo 1-1Nik strain has been provided with GenBank accession number MW326085 (for nucleotide sequence SUB8683960 Seq1). EMo 1-1Nik strain is hemolysis-negative (γ -hemolysis), DNase negative and gelatinase negative; absent of virulence factor genes, low-grade bio lm-positive (0.133 ± 0.36), mostly susceptible to tested antibiotics. Moreover, 60% of EMo1-1Nik colonies were found as bacteriocin-producing against the principal indicator Enterococcus avium EA5. EMo1-1Nik concentrated substance pH 4.5 (CS) showed the inhibitory activity against EA5 strain (200 AU/mL); CSs with pH 6.3 and 7.3 reached inhibitory activity 100 AU/mL against EA5 strain.Oppositelly, EMo 1-1Nik was susceptible to Mundticin EM41/3 (800 AU/mL) produced by fecal horses strain E. mundtii EM41/3. In spite of the preliminary results, it has been shown bacteriocin potential of safe E. moraviensis EMo1-1Nik strain. The additional studies are in processing.

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Enterocin M-Producing Enterococcus faecium CCM 8558 Demonstrating Probiotic Properties in Horses

Cited by 11 publications

References 25 publications

Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits

Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits

Gut Microbiota Manipulation in Foals—Naturopathic Diarrhea Management, or Unsubstantiated Folly?

Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain

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