Enterocin M in Interaction in Broiler Rabbits with Autochthonous, Biofilm-Forming Enterococcus hirae Kr8 Strain

Lauková, Andrea; Chrastinová, Ľubica; Micenková, Lenka; Bino, Eva; Kubašová, Ivana; Kandričáková, Anna; Gancarčíková, Soňa; Plachá, Iveta; Holodová, Monika; Grešaková, Ľubomíra; Formelová, Z.; Simonová, Monika Pogány

doi:10.1007/s12602-022-09941-5

Cited by 2 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the biofilm-forming ability of the Kr8 + strain as a virulence factor, we hypothesized a “Kr8 + attack” on the health and performance of rabbits and a lower tolerance of the host organism to this strain. However, based on higher weight gains, we can assume slower biofilm formation and/or a stimulated immune response in the hostile environment to defend against the spoilage/pathogen agent [ 27 ]. On the other hand, the lowest FCR was noted in the EG2 group that administered Ent M ( P < 0.001).…”

Section: Discussionmentioning

confidence: 99%

Application of Autochthonous Biofilm-Forming Enterococcus hirae Kr8 Strain in Relation with Enterocin M in Broiler Rabbits and Their Effect on the Rabbit Meat Quality: Risk or Protection?

Simonová

Chrastinová

Bino

et al. 2023

Probiotics & Antimicro. Prot.

Self Cite

View full text Add to dashboard Cite

Around weaning, rabbits are sensitive to gastrointestinal diseases, mostly of bacterial origin, including enterococci (Enterococcus hirae), clostridia, and coliforms. Preventive use of postbiotics—enterocins—as feed additives can reduce this problem. Therefore, simulation of spoilage/pathogenic environment applying the autochthonous, biofilm-forming E. hirae Kr8+ strain in rabbits and its influence on rabbit meat quality as well as the protective effect of Ent M on rabbit meat properties and quality in infected animals was tested. Ninety-six rabbits aged 35 days, both sexes, meat line M91 breed were divided into one control (CG) and three experimental (EG1, EG2, and EG3) groups. The rabbits in CG received standard diet, without any additives, rabbits in EG1 received 108 CFU/mL of Kr8+ strain (at a dose of 500 μL/animal/day), to rabbits in EG2 the Ent M (50 μL/animal/day), and in EG3, combination of the Kr8+ and Ent M was applied in their drinking water during 21 days. The experiment lasted 42 days. The Kr8+ strain did not attack the gastrointestinal tract and have any adverse effect on the meat quality of rabbits. Moreover, improved weight gains, carcass parameters, and higher essential fatty acid (EAA) and amino acid (EAA) content of rabbit meat point rather to its possible beneficial potential in rabbit nutrition. Administration of Ent M improved most of the tested parameters: animal weight and meat physicochemical and nutritional properties, with a focus on EFA and EAA. During combination of both additives, their synergistic impact was noted, improving the nutritional quality, mostly the EAA content of rabbit meat.

show abstract

Section: Discussionmentioning

confidence: 99%

Application of Autochthonous Biofilm-Forming Enterococcus hirae Kr8 Strain in Relation with Enterocin M in Broiler Rabbits and Their Effect on the Rabbit Meat Quality: Risk or Protection?

Simonová

Chrastinová

Bino

et al. 2023

Probiotics & Antimicro. Prot.

Self Cite

View full text Add to dashboard Cite

show abstract

The effect of enterocin A/P dipeptide on growth performance, glutathione-peroxidase activity, IgA secretion and jejunal morphology in rabbits after experimental methicillin-resistant Staphylococcus epidermidis P3Tr2a Infection

Pogány Simonová,

Chrastinová,

Ščerbová

et al. 2023

Vet Res Commun

View full text Add to dashboard Cite

The increasing frequency of methicillin-resistant (MR) staphylococci in humans and animals need special attention for their difficult treatment and zoonotic character, therefore novel antimicrobial compounds on a natural base against antibiotic-resistant bacteria are requested. Currently, bacteriocins/enterocins present a new promising way to overcome this problem, both in prevention and treatment. Therefore, the preventive and medicinal effect of dipeptide enterocin EntA/P was evaluated against MR Staphylococcus epidermidis SEP3/Tr2a strain in a rabbit model, testing their influence on growth performance, glutathione-peroxidase (GPx) enzyme activity, phagocytic activity (PA), secretory (s)IgA, and jejunal morphometry (JM). Eighty-eight rabbits (aged 35 days, meat line M91, both sexes) were divided into experimental groups S (SEP3/Tr2a strain; 1.0 × 105 CFU/mL; dose 500µL/animal/day for 7 days, between days 14 and 21 to simulate the pathogen attack), E (EntA/P; 50 µL/animal/day, 25,600 AU/mL in two intervals, for preventive effect between days 0 and 14; for medicinal effect between days 28 and 42), E + S (EntA/P + SEP3/Tr2a; preventive effect; SEP3/Tr2a + EntA/P; medicinal effect) and control group (C; without additives). Higher body weight was recorded in all experimental groups (p < 0.001) compared to control data. The negative influence/attack of the SEP3Tra2 strain on the intestinal immunity and environment was reflected as decreased GPx activity, worse JM parameters and higher sIgA concentration in infected rabbits. These results suggest the promising preventive use of EntA/P to improve the immunity and growth of rabbits, as well as its therapeutic potential and protective role against staphylococcal infections in rabbit breeding.

show abstract

Enterocin M in Interaction in Broiler Rabbits with Autochthonous, Biofilm-Forming Enterococcus hirae Kr8 Strain

Cited by 2 publications

References 30 publications

Application of Autochthonous Biofilm-Forming Enterococcus hirae Kr8 Strain in Relation with Enterocin M in Broiler Rabbits and Their Effect on the Rabbit Meat Quality: Risk or Protection?

Application of Autochthonous Biofilm-Forming Enterococcus hirae Kr8 Strain in Relation with Enterocin M in Broiler Rabbits and Their Effect on the Rabbit Meat Quality: Risk or Protection?

The effect of enterocin A/P dipeptide on growth performance, glutathione-peroxidase activity, IgA secretion and jejunal morphology in rabbits after experimental methicillin-resistant Staphylococcus epidermidis P3Tr2a Infection

Contact Info

Product

Resources

About