Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia

Peng, Guixiang; Zhang, Wu; LUO, HONGMEI; Xie, Haizhu; Lai, Weihao; Tan, Zhi Yuan

doi:10.1099/ijs.0.65484-0

Cited by 30 publications

(29 citation statements)

References 33 publications

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“…It was interesting to note that the DNA-DNA relatedness values between E. radicincitans LMG 23767 T and E. oryzae LMG 24251 T (79% ±6) and between E. radicincitans LMG 23767 T and E. arachidis LMG 26131 T (66% ±17), in our experiments, were much higher than those reported by the original authors [3]. Support for the robustness of our data is provided by the phylogenetic relationships revealed by the rpoB gene sequences, where E. radicincitans D5/23 T and E. arachidis Ah-143 T were 98.9% similar.…”

Section: Resultscontrasting

confidence: 68%

“…For instance, rice growth under conditions of low temperature, high salinity or desiccation may be favored. Moreover, endophytes can supply nitrogen to rice tissues [3]. In previous work, different bacteria, in particular belonging to the enterics, have been isolated from rice seeds [4,5], roots [3,6] and stems [7].…”

Section: Introductionmentioning

confidence: 99%

“…CBMB30, which have been isolated from respectively the phyllosphere of wheat, the rhizosphere of groundnut and the endosphere of rice species (i.e. Oryza latifolia and O. sativa ), can improve the fitness of their host plants and are therefore known as plant-growth-promoting bacteria (PGPB; [3,12,13]).…”

Section: Introductionmentioning

confidence: 99%

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The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

et al. 2013

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BackgroundSix independent Gram-negative, facultatively anaerobic, non-spore-forming, nitrogen-fixing rod-shaped isolates were obtained from the root endosphere of rice grown at the International Rice Research Institute (IRRI) and investigated in a polyphasic taxonomic study.ResultsThe strains produced fatty acid patterns typical for members of the family Enterobacteriaceae. Comparative sequence analyses of the 16S rRNA as well as rpoB genes allocated the strains to two well-defined groups within the genus Enterobacter, family Enterobacteriaceae. The analyses indicated Enterobacter radicincitans, Enterobacter arachidis and Enterobacter oryzae to be the closest related species. An RpoB (translated) protein comparison supported the placement in the genus Enterobacter and the relatedness of our isolates to the aforementioned species. Genomic DNA:DNA hybridization analyses and biochemical analyses provided further evidence that the novel strains belong to two new species within the genus Enterobacter. The two species can be differentiated from each other and from existing enteric species by acid production from L-rhamnose and D-melibiose, decarboxylation of ornithine and utilization of D-alanine, D-raffinose L-proline and L-aspartic acid, among other characteristics. Members of both species revealed capacities to colonise rice roots, including plant-growth-promoting capabilities such as an active supply of fixed nitrogen to the plant and solubilisation of inorganic phosphorus, next to traits allowing adaptation to the plant.ConclusionsTwo novel proposed enterobacterial species, denominated Enterobacter oryziphilus sp. nov. (type strain REICA_142T=LMG 26429T=NCCB 100393T) and Enterobacter oryzendophyticus sp. nov. (type strain REICA_082T=LMG 26432T =NCCB 100390T) were isolated from rice roots. Both species are capable of promoting rice growth by supplying nitrogen and phosphorus.

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Section: Resultscontrasting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

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The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

et al. 2013

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“…Both of the latter are nitrogen fixers (e.g. Chelius and Triplett 2000;Peng et al 2009). No reports exist of the diazotrophic nature of R. ornithinolytica (formerly Klebsiella ornithinolytica), nor did we detect the nifH gene, despite evidence of nitrogenase activity.…”

Section: Discussionmentioning

confidence: 52%

Nitrogen fixation associated with sago (Metroxylon sagu) and some implications

Shipton

Baker

Blaney³

et al. 2010

Letters in Applied Microbiology

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Aims: To determine the presence and contribution of diazotrophic bacteria to nitrogen concentrations in edible starch derived from the sago palm (Metroxylon sagu). Methods and Results: Isolation of diazotrophic bacteria and analysis of nitrogen fixation were conducted on pith, root and sago starch samples. Acetylene reduction showed that five of ten starch samples were fixing nitrogen. Two presumptive nitrogen‐fixing bacteria from starch fixed nitrogen in pure culture and five isolates were positive for the nif H gene. Nitrogen concentrations in 51 starch samples were low (37 samples <0·2 g kg−1; 14 ranging from 0·2 to 2·0 g kg−1). Conclusions: Nitrogen fixation occurs in sago starch, which undoubtedly plays a role in fermentation ecology. Nitrogen levels are considered too low to be of nutritional benefit and to protect against nutritional‐associated illnesses. Significance and Impact of the Study: Sago starch does not add significantly to the protein calorie intake and may be associated with susceptibility to nutritional‐associated illness.

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“…The Enterobacter species are gram-negative bacteria belonging to the family Enterobacteriaceae, and the genus Enterobacter has been one of the most rapidly expanding genera in the last decade [12]. Members of this genus are most commonly isolated from the environment, in particular from soil, plants, and fruits, but also from human sources [13]. Several strains of Enterobacter have been investigated as plant growth-promoting rhizobacteria that colonize plant roots and improve the health or yields of plants [14,15].…”

Section: Introductionmentioning

confidence: 99%

Nematicidal and Plant Growth-Promoting Activity of Enterobacter asburiae HK169: Genome Analysis Provides Insight into Its Biological Activities

Oh¹,

Han²,

Lee³

et al. 2018

Journal of Microbiology and Biotechnology

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In the course of screening for microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against the root-knot nematode Meloidogyne incognita, along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% while also increasing root and shoot weights by 251% and 160%, respectively, compared with an untreated control. The cell-free culture filtrate of the HK169 culture killed all juveniles of M. incognita within 48 h. In addition, the nematicidal activity of the culture filtrate was dramatically reduced by a protease inhibitor, suggesting that proteolytic enzymes contribute to the nematicidal activity of HK169. In order to obtain genomic information about the HK169 isolate related to its nematicidal and plant growth-promoting activities, we sequenced and analyzed the whole genome of the HK169 isolate, and the resulting information provided evidence that the HK169 isolate has nematicidal and plant growth-promoting activities. Taken together, these observations enable the future application of E. asburiae HK169 as a biocontrol agent for nematode control and promote our understanding of the beneficial interactions between E. asburiae HK169 and plants.

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Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia

Cited by 30 publications

References 33 publications

The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

Nitrogen fixation associated with sago (Metroxylon sagu) and some implications

Nematicidal and Plant Growth-Promoting Activity of Enterobacter asburiae HK169: Genome Analysis Provides Insight into Its Biological Activities

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