Enterobacter asburiae E7, a Novel Potential Probiotic, Enhances Resistance to Aeromonas veronii Infection via Stimulating the Immune Response in Common Carp (
            <i>Cyprinus carpio</i>
            )

Li, Jing; Zhang, Zhao; Wu, Zhibin; Qu, Shenye; Wang, Gao‐Xue; Wei, Dongdong; Li, Pengfei; Ling, Fei

doi:10.1128/spectrum.04273-22

Cited by 8 publications

(3 citation statements)

References 78 publications

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“…Meanwhile, Enterobacter spp. has been demonstrated as a potential probiotic for aquaculture, exhibiting extensive resistance to bacterial infections, lacking pathogenicity toward the host, and showcasing robust environmental tolerance [ 42 ]. In a recent study by Tang et al, E .…”

Section: Discussionmentioning

confidence: 99%

Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh

Kawser,

Hoque,

Rahman

et al. 2024

PLoS ONE

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The field of fish microbiome research has rapidly been advancing, primarily focusing on farmed or laboratory fish species rather than natural or marine fish populations. This study sought to reveal the distinctive gut bacteriome composition and diversity within the anadromous fish species Tenualosa ilisha (hilsa), which holds the status of being the national fish of Bangladesh. We conducted an analysis on 15 gut samples obtained from 15 individual hilsa fishes collected from three primary habitats (e.g., freshwater = 5, brackish water = 5 and marine water = 5) in Bangladesh. The analysis utilized metagenomics based on 16S rRNA gene sequencing targeting the V3-V4 regions. Our comprehensive identification revealed a total of 258 operational taxonomic units (OTUs). The observed OTUs were represented by six phyla, nine classes, 19 orders, 26 families and 40 genera of bacteria. Our analysis unveiled considerable taxonomic differences among the habitats (freshwater, brackish water, and marine water) of hilsa fishes, as denoted by a higher level of shared microbiota (p = 0.007, Kruskal-Wallis test). Among the identified genera in the gut of hilsa fishes, including Vagococcus, Morganella, Enterobacter, Plesiomonas, Shigella, Clostridium, Klebsiella, Serratia, Aeromonas, Macrococcus, Staphylococcus, Proteus, and Hafnia, several are recognized as fish probiotics. Importantly, some bacterial genera such as Sinobaca, Synechococcus, Gemmata, Serinicoccus, Saccharopolyspora, and Paulinella identified in the gut of hilsa identified in this study have not been reported in any aquatic or marine fish species. Significantly, we observed that 67.50% (27/40) of bacterial genera were found to be common among hilsa fishes across all three habitats. Our findings offer compelling evidence for the presence of both exclusive and communal bacteriomes within the gut of hilsa fishes, exhibiting potential probiotic properties. These observations could be crucial for guiding future microbiome investigations in this economically significant fish species.

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Section: Discussionmentioning

confidence: 99%

Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh

Kawser,

Hoque,

Rahman

et al. 2024

PLoS ONE

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“…Enterobacter spp. has already been proved as a prospective probiotic for aquaculture applications, and showed extensive resistance to bacterial infection, no pathogenicity to the host, and stronger environmental tolerance 25 . In a recent study, Tang et al isolated E. asburiae C28 from the intestine of Carassius auratus, which reduced the load of potential pathogens, increased the number of potential probiotics in the host gut, and decreased the death rate of C. auratus challenged by A. hydrophila 26 .…”

Section: Discussionmentioning

confidence: 99%

Unveiling the Gut and Flesh Microbiome Signature and Diversity of the Bangladesh National Fish Hilsa (Tenualosa ilisha)

Hoque,

Kawser,

Rahman

et al. 2023

Preprint

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Fish microbiome science is progressing fast, but it is mostly restricted to farmed or laboratory fish species compared to natural or marine fish populations. The aim of this study was to unveil the gut and flesh microbiome signature and diversity of the anadromous fish, Tenualosa ilisha (hilsa), the national fish of Bangladesh. We analysed 18 samples including 15 gut (intestinal contents) and three flesh samples from 18 individual hilsa fishes collected from three major habitats (e.g., freshwater, brackish water and marine water) of hilsa in Bangladesh through 16S rRNA (V3 – V4 region) gene sequencing-based metagenomics. A total of 325 operational taxonomic units (OTUs) including 67 and 258 in flesh and gut samples, respectively were identified. The observed OTUs were represented by six phyla, nine classes, 19 orders, 26 families and 40 genera of bacteria. Our findings revealed substantial taxonomic variability between sample categories (i.e., gut and flesh; p = 0.0127; Kruskal Wallis test), and habitats (freshwater, brackish water, and marine water; (p = 0.007; Kruskal Wallis test) of the hilsa fishes, indicated by their higher degree of shared microbiota. Of the identified genera, Vagococcus, Morganella, Enterobacter, Plesiomonas, Shigella, Clostridium, Klebsiella, Serratia, Aeromonas, Macrococcus, Staphylococcus, Proteus, Hafnia etc. were the major bacterial genera detected in hilsa fish. Importantly, we detected six genera such as Sinobaca, Synechococcus, Gemmata, Serinicoccus, Saccharopolyspora, and Paulinella seem to be specific to the hilsa fishes. Our data provided evidence for the existence of both unique and shared bacteriomes with probiotics potentials in the gut and flesh of hilsa fishes, which might be taken into consideration for undertaking future microbiome study in this economically important fish species.

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“…However, with the rapid development of aquaculture, environmental pollution and aquatic product quality and safety issues are becoming increasingly prominent (Andrade et al, 2023). In the process of aquaculture, intensive stocking, excessive baiting, illegal use of drugs and arbitrary discharge of farming wastewater occur from time to time, resulting in more serious pollution of farming waters, leading to a steep rise in the outbreak of diseases in farmed animals, which not only brings serious economic losses but also causes great damages to the water ecosystem (Li et al, 2023).…”

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confidence: 99%

Phenotypic characteristics and immune response of Procypris merus following challenge with aquatic isolate of Klebsiella pneumoniae

Liao,

Wei,

Liu

et al. 2023

Journal of Fish Diseases

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Currently, aquaculture is a relatively mature industry; however, disease problems are continuously threatening the industry and hindering its development to a certain extent. Klebsiella pneumoniae is one of the zoonotic bacteria widely present in different hosts and has caused some degree of harm to the aquaculture industry, posing a potential threat to the water environment and indirectly also affecting human food safety issues. In this study, K. pneumoniae was isolated from the aquaculture environment, named as ELD, and subjected to pathogenic and immunological related studies. The results of the study showed that the strain carries at least four virulence‐related genes, magA, wabG, ureA and uge, and has developed resistance to at least seven antibacterial drugs, such as amoxicillin, doxycycline, rifampicin, and so on. Moreover, the strain is highly pathogenic and is capable of causing systemic clinical foci in Procypris merus. In addition, after infection with K. pneumoniae, the expression of IL‐1β, IL‐8, HSP70 and C2 was upregulated in P. merus as a whole, whereas the expression of TNF‐α did not change significantly in any of the tissues, which might be a kind of immune response of P. merus against K. pneumoniae infection. This study provides an important theoretical basis for the in‐depth exploration of the pathogenic mechanism of K. pneumoniae in fish and the immune response that occurs after the disease is contracted in fish, as well as theoretical support for the development of effective preventive and therapeutic strategies against K. pneumoniae‐infected aquatic animals in the future.

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Enterobacter asburiae E7, a Novel Potential Probiotic, Enhances Resistance to Aeromonas veronii Infection via Stimulating the Immune Response in Common Carp ( Cyprinus carpio )

Abstract: In the present study, we evaluated for the first time the efficiency of Enterobacter asburiae as a prospective probiotic for aquaculture applications. The E7 strain showed extensive resistance to Aeromonas , no pathogenicity to the host, and stronger environmental tolerance.

Cited by 8 publications

References 78 publications

Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh

Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh

Unveiling the Gut and Flesh Microbiome Signature and Diversity of the Bangladesh National Fish Hilsa (Tenualosa ilisha)

Phenotypic characteristics and immune response of Procypris merus following challenge with aquatic isolate of Klebsiella pneumoniae

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