Enteroaggregative Escherichia coli from humans and animals differ in major phenotypical traits and virulence genes

Uber, Ana Paula; Trabulsi, Luíz Rachid; Irino, Kinue; Beutin, Lothar; Ghilardi, Angela C. R.; Gomes, Tânia A. T.; Liberatore, Ana Maria Alvim; Castro, Antonio Fernando Pestana de; Elias, Waldir P.

doi:10.1111/j.1574-6968.2006.00124.x

Cited by 68 publications

(51 citation statements)

References 34 publications

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“…Remarkably, individuals with an AA rather than an AT or TT genotype at the Ϫ251 position of the interleukin-8 (IL-8) gene generated greater fecal IL-8 in response to EAEC and were associated with increased infection with EAEC (601). While atypical EAEC has also been identified in calves, piglets, and horses, animals are not an important reservoir of human-pathogenic typical EAEC (602). However, because EAEC isolates are so heterogeneous, it is premature to rule out animal reservoirs for undercharacterized lineages of EAEC.…”

Section: Epidemiologymentioning

confidence: 99%

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

et al. 2013

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SUMMARY Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. Pathogenic variants of E. coli (pathovars or pathotypes) cause much morbidity and mortality worldwide. Consequently, pathogenic E. coli is widely studied in humans, animals, food, and the environment. While there are many common features that these pathotypes employ to colonize the intestinal mucosa and cause disease, the course, onset, and complications vary significantly. Outbreaks are common in developed and developing countries, and they sometimes have fatal consequences. Many of these pathotypes are a major public health concern as they have low infectious doses and are transmitted through ubiquitous mediums, including food and water. The seriousness of pathogenic E. coli is exemplified by dedicated national and international surveillance programs that monitor and track outbreaks; unfortunately, this surveillance is often lacking in developing countries. While not all pathotypes carry the same public health profile, they all carry an enormous potential to cause disease and continue to present challenges to human health. This comprehensive review highlights recent advances in our understanding of the intestinal pathotypes of E. coli .

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Section: Epidemiologymentioning

confidence: 99%

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

et al. 2013

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“…The pAA plasmid contains several virulence genes, including aggR, aap and aat, but not all EAEC carry the same complement of such genes. While "typical" EAEC strains, such as VT-producing EAEC O104:H4, carry the pAA plasmid or at least the aggR gene, "atypical" strains lack the pAA plasmid and the aggR gene, though they still adhere to the HEp-2 cells in a stacked-brick pattern (Uber et al 2006, Weintraub 2007, EFSA 2011. EAEC appear to have adapted specifically to the human host (Wieler et al 2011): typical EAEC have not been found in animals, and atypical EAEC, although identified in calves, piglets and horses (Uber et al 2006), are not considered zoonotic pathogens (Okhuysen and DuPont 2010).…”

Section: Introductionmentioning

confidence: 99%

Ruminants are not a reservoir of enteroaggregative Escherichia coli

Orden

Domínguez‐Bernal

Horcajo

et al. 2017

Austral j. vet. sci.

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ABSTRACT. Enteroaggregative Escherichia coli (EAEC) causes both acute and persistent diarrhoea among children and adults in developing and developed countries. In addition, the large outbreak of food-borne illness in Europe in the summer of 2011 was caused by a verotoxin-producing EAEC O104:H4 strain. The public health threat posed by EAEC makes it important to identify animal reservoirs that might lead to epidemics in humans. Based on only one study in sheep and a few in cattle, researchers have suggested that EAEC are not found in ruminants. To expand the available data on this question, the present study applied polymerase chain reaction to faecal samples from 920 cattle, sheep and goats to detect EAEC. None of the samples tested positive for any of the EAEC genes tested, suggesting that goats, like cattle and sheep, are unlikely to be a significant reservoir of EAEC.Key words: enteroaggregative E. coli, ruminants. RESUMEN.Las Escherichia coli enteroagregativas (ECEA) causan tanto diarrea aguda como persistente en niños y adultos en países desarrollados y en vías de desarrollo. Además, el gran brote de transmisión alimentaria que sucedió en el verano de 2011 fue causado por una cepa de ECEA O104:H4 productora de verotoxinas. La amenaza a la Salud Pública debida a las ECEA hace importante identificar los reservorios animales a partir de los cuales se podrían originar epidemias o brotes en humanos. Basados en un único estudio en ovejas y unos pocos en vacas, algunos investigadores han sugerido que los ECEA no se aíslan de rumiantes. Para ampliar los datos disponibles acerca de esta cuestión, en este estudio se usó la reacción en cadena de la polimerasa para detectar ECEA en 920 muestras fecales de vacas, ovejas y cabras. Ninguna de las muestras resultó positiva a los genes de ECEA analizados, sugiriendo que las cabras, al igual que las vacas y ovejas, no son probablemente un reservorio importante de ECEA.

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“…Thus, since this survey used a strain collection that is likely to hold pathogenic, nonpathogenic commensals and possible cross-contaminating isolates, the significance of this finding is unclear. Recently, Uber et al (35) characterized a collection of EAEC strains of human and animal specimens in order to investigate a possible role of animals as reservoir for EAEC human infections. In their work they included some of the EAEC strains used in the present study.…”

Section: Discussionmentioning

confidence: 99%

Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses

Liberatore¹,

Tomita²,

Vieira³

et al. 2007

Braz. J. Microbiol.

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The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings) was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2%) adhered to HeLa cells, 21 (77.8%) of which presented the aggregative adherence pattern (AA) that characterize the Enteroaggregative E. coli pathotype (EAEC). Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence genes of the EAEC pathotype (aggR, aap, irp2, and pic). This is the first description of the AA phenotype amongst E. coli strains from sick horses. Such strains should be further evaluated regarding their potential role in the pathogenesis of diverse equine diseases and as reservoirs of human infections.

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Enteroaggregative Escherichia coli from humans and animals differ in major phenotypical traits and virulence genes

Cited by 68 publications

References 34 publications

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

Ruminants are not a reservoir of enteroaggregative Escherichia coli

Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses

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