Enteric Dysbiosis is Associated with Sepsis in Patients

Liu, Zhan‐Guo; Li, Na; Fang, Heng; Guo, Yuexun; Gong, Shenhai; Niu, Mengwei; Chang, Ping; Chen, Peng

doi:10.2139/ssrn.3279177

Cited by 14 publications

(14 citation statements)

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“…The intestinal tract is the location of secondary infection in sepsis patients. Liu's study confirmed that sepsis is associated with disruption of gut microbial compositional [13]. Generally, the intestinal microbiota is inevitably affected by antibiotic treatment.…”

Section: Discussionmentioning

confidence: 84%

“…Intestinal dysbacteriosis is being studied increasingly in the pathogenesis of immune-related diseases such as tumorigenesis, metabolic diseases, and autoimmune disease. Liu et al [13] reported that enteric dysbiosis was associated with sepsis. Li et al reported that, after seven days of fecal transplantation, the small intestine CD4+, CD8+, B220+ cells, colon CD4+ and part CD8+ cells were restored from abnormalities induced by long-term use of antibiotics [14].…”

Section: Introductionmentioning

confidence: 99%

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Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

Zhao¹,

Chen²,

Wu³

et al. 2020

Preprint

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Background Our understanding of sepsis-associated immune impairment is incomplete. The objective of this retrospective study was to investigate correlations of sepsis clinical manifestations with peripheral blood lymphocyte subpopulations in lymphocyte immunity. Methods Twenty individuals without sepsis and eighteen with sepsis were enrolled. Lymphocyte phenotypes (CD3+, CD4+, CD8+, CD3-CD16+CD56+, CD19+, CD4+CD25+CD127+, CD8+CD28-, and CD8+CD28+) were assessed by flow cytometry. Fresh fecal bacteria cue proportion was measured to determine intestinal dysbacteriosis. Results Compared with the non-sepsis group, the sepsis patients had clearly lower proportions of CD3+, CD4+ and CD8+CD28+ cells and substantially higher proportions of CD19+ cells (p<0.05). Among 38 patients with infection, CD4+ cells and CD8+CD28+ cells in a survivor group had significantly higher presence compared with patients who had died (p<0.05), The subgroup analysis results showed that CD4+ cells in the survivor subgroups were higher than those in the deceased subgroups (p<0.05). CD8+CD28+ cells in the non-sepsis survivor subgroup were higher than those in the deceased subgroups (p<0.05).. Bivariate correlation analysis showed that the intestinal dysbacteriosis was significantly correlated with the severity of sepsis and its prognosis (r2=0.2788, p=0.001, r2=0.1764, p=0.009, respectively). CD4+, CD19+, and CD8+CD28+ cells were significantly correlated with intestinal dysbacteriosis (r2=0.1024, p=0.049, r2=0.1063, p=0.046, r2=0.1909, p=0.006, respectively). Conclusions In conclusion, the lymphocyte populations of CD3+, CD4+, CD8+CD28+ and CD19+ cells were accessible for predicting the severity and mortality of sepsis patients. In addition, intestinal dysbacteriosis had a significant impact on the immune system of sepsis patients as revealed by peripheral blood lymphocyte population.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

Zhao¹,

Chen²,

Wu³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The syndrome can be induced by a wide variety of microbes and, by definition, involves a maladaptive response to a pathogen 5 . The gastrointestinal (GI) tract has long been hypothesized to play an integral role in the pathophysiology of sepsis, by both driving and perpetuating multiple organ dysfunction 8,9 . The original concept of gut-derived sepsis proposed that the altered inflammatory milieu induced by overwhelming infection leads to intestinal hyperpermeability, allowing luminal contents, including intact microbes, and microbial products, to escape their natural environment where they can cause either local or distant injury 1,5,6,10 .…”

Section: The World Healthmentioning

confidence: 99%

Fecal microbiota transplantation reconstructs the gut microbiota of septic mice and protects the intestinal mucosal barrier

Gai

Wang

et al. 2020

Preprint

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The gastrointestinal (GI) tract has long been hypothesized to play an integral role in the pathophysiology of sepsis, and gut microbiota (GM) dysbiosis may be the key factor. Previous studies has confirmed that microbiome is markedly altered in critical illness. We aimed to confirm the existence of gut microbiota imbalance in the early stage of sepsis, observe the effect of fecal microbiota transplantation (FMT) on sepsis, and explore whether FMT can reconstruct the GM of septic mice and restore its protective function on the intestinal mucosal barrier. Through the study of flora, mucus layer, tight junction, immune barrier, and short-chain fatty acid changes in septic mice and fecal microbiota transplanted mice , we found that GM imbalance exists early in sepsis. FMT can improve morbidity and effectively reduce mortality in septic mice. After the fecal bacteria were transplanted, the abundance and diversity of the gut flora were restored, and the microbial characteristics of the donors changed. FMT can effectively reduce epithelial cell apoptosis, improve the composition of the mucus layer, upregulate the expression of tight junction proteins, and reduce intestinal permeability and the inflammatory response, thus protecting the intestinal barrier function. After FMT, Lachnospiraceae contributes the most to intestinal protection through enhancement of the L-lysine fermentation pathway, resulting in the production of acetate and butanoate, and may be the key bacteria for short-chain fatty acid metabolism and FMT success.

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“…In view of the well‐known interactions between the microbiota and the immune system, the implications during sepsis are probably major, although understanding remains especially challenging. By modulation of the immune system, gut microbiota can contribute to systemic inflammation 49 both by induction of cytokine response and by means of releasing certain metabolites to the systemic circulation 49 …”

Section: Microbiome In Sepsismentioning

confidence: 99%

The clinical relevance of the microbiome when managing paediatric infectious diseases—Narrative review

et al. 2020

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In recent years, the field of infectious diseases has been hit by the overwhelming amount of information generated while the human microbiome is being disentangled. Based on the interaction between the microbiota and the immune system, the implications regarding infectious diseases are probably major and remain a challenge. Aims This review was conceived as a comprehensive tool to provide an overview of the available evidence regarding the influence of the microbiome on infectious diseases in children. Methods We present the main findings aroused from microbiome research in prevention, diagnosis and treatment of infectious disease under a paediatric perspective, to inform clinicians of the potential relevance of microbiome‐related knowledge for translation to clinical practice. Results and Conclusion The evidence shown in this review highlights the numerous research gaps ahead and supports the need to move forward to integrating the so‐called microbiome thinking into our routine clinical practice.

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Enteric Dysbiosis is Associated with Sepsis in Patients

Cited by 14 publications

References 0 publications

Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

Fecal microbiota transplantation reconstructs the gut microbiota of septic mice and protects the intestinal mucosal barrier

The clinical relevance of the microbiome when managing paediatric infectious diseases—Narrative review

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