Enteral yeast-selenium supplementation in preterm infants

G, Bogye; Alfthan, Georg; Machay, T; Zubovics, László

doi:10.1136/fn.78.3.f225

Cited by 13 publications

(10 citation statements)

References 4 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is well accepted that Se bioavailability studies need to assess absorption and retention in animals/humans but also need to measure the selenoprotein functionality such as GPx activity [13,14,18,19]. The enzymatic properties of gastrointestinal GPx (GPx2) are nearly indistinguishable from cytosolic GPx (GPx1), their physical properties are similar, and the production of both is dependent on the supply of Se [28].…”

Section: Discussionmentioning

confidence: 99%

“…The compounds available for use as Se supplements include the inorganic forms, sodium selenite and sodium selenate, and the organic forms, SeMet and Se-enriched yeast [11]. Although the metabolism of both organic and inorganic Se forms shows certain similarities, not all of these forms are metabolized alike, and humans have been found to absorb and retain Se better from SeMet than from the inorganic Se salts [12][13][14]. Unlike the organic forms of Se, in which Se is in the reduced state (selenide: Se 2− ), the inorganic salts contain Se in oxidized forms (selenite: Se 4+ ; selenate: Se 6+ ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Selenium-deficient Caco-2 Cell Model for Assessing Differential Incorporation of Chemical or Food Selenium into Glutathione Peroxidase

Zeng

Botnen

Johnson

2008

Biol Trace Elem Res

View full text Add to dashboard Cite

Assessing the ability of a selenium (Se) sample to induce cellular glutathione peroxidase (GPx) activity in Se-deficient animals is the most commonly used method to determine Se bioavailability. Our goal is to establish a Se-deficient cell culture model with differential incorporation of Se chemical forms into GPx, which may complement the in vivo studies. In the present study, we developed a Se-deficient Caco-2 cell model with a serum gradual reduction method. It is well recognized that selenomethionine (SeMet) is the major nutritional source of Se; therefore, SeMet, selenite, or methylselenocysteine (SeMSC) was added to cell culture media with different concentrations and treatment time points. We found that selenite and SeMSC induced GPx more rapidly than SeMet. However, SeMet was better retained as it is incorporated into proteins in place of methionine; compared with 8-, 24-, or 48-h treatment, 72-h Se treatment was a more sensitive time point to measure the potential of GPx induction in all tested concentrations. Based on induction of GPx activity, the cellular bioavailability of Se from an extract of selenobroccoli after a simulated gastrointestinal digestion was comparable with that of SeMSC and SeMet. These in vitro data are, for the first time, consistent with previous published data regarding selenite and SeMet bioavailability in animal models and Se chemical speciation studies with broccoli. Thus, Se-deficient Caco-2 cell model with differential incorporation of chemical or food forms of Se into GPx provides a new tool to study the cellular mechanisms of Se bioavailability.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Selenium-deficient Caco-2 Cell Model for Assessing Differential Incorporation of Chemical or Food Selenium into Glutathione Peroxidase

Zeng

Botnen

Johnson

2008

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…Intravenous selenium administration improves the outcomes for preterm infants born in selenium-deficient areas [69,70]. Placebo controlled clinical trials also increase the fertility of men living in low selenium areas leading to successful conception [71].…”

Section: Clinical Studies With Dietary Seleniummentioning

confidence: 99%

Dietary selenium and selenoprotein function

2012

View full text Add to dashboard Cite

SummarySelenium is a trace mineral and an essential nutrient in the human diet. Selenium is found in soil and water and consequently enters the food chain through the root ways of plants and aquatic organisms. Some areas of the world are low in soil selenium resulting in a selenium deficient population and the appearance of an associated heart disease and bone disorders that can be corrected with dietary selenium. Indeed the requirement for dietary selenium was established by these observations and while selenium deficiency is rare in the West, patients requiring long-term intravenous feedings have also show heart disease associated with a deficiency of selenium in the feeding fluids. Subsequently, it has been established that dietary selenium can improve a wide range of human health conditions even in areas with soil replete in selenium.

show abstract

“…Upon absorption of these compounds, the highervalence forms are reduced to the selenide with state using reducing equivalents from reduced glutathione and NADPH, while the organic forms such as SeMet and SeCys release Se in the selenide state as a result of catabolism [3,4]. Humans absorb and retain Se better from SeMet than from most of other Se chemical forms; however, SeMet is less able to replete selenoprotein expression [7,8].…”

Section: Introductionmentioning

confidence: 98%

Chemical Form of Selenium Affects Its Uptake, Transport, and Glutathione Peroxidase Activity in the Human Intestinal Caco-2 Cell Model

Zeng

Jackson

Cheng

et al. 2010

Biol Trace Elem Res

View full text Add to dashboard Cite

Determining the effect of selenium (Se) chemical form on uptake, transport, and glutathione peroxidase activity in human intestinal cells is critical to assess Se bioavailability at nutritional doses. In this study, we found that two sources of L-selenomethionine (SeMet) and Se-enriched yeast each increased intracellular Se content more effectively than selenite or methylselenocysteine (SeMSC) in the human intestinal Caco-2 cell model. Interestingly, SeMSC, SeMet, and digested Se-enriched yeast were transported at comparable efficacy from the apical to basolateral sides, each being about 3-fold that of selenite. In addition, these forms of Se, whether before or after traversing from apical side to basolateral side, did not change the potential to support glutathione peroxidase (GPx) activity. Although selenoprotein P has been postulated to be a key Se transport protein, its intracellular expression did not differ when selenite, SeMSC, SeMet, or digested Se-enriched yeast was added to serum-contained media. Taken together, our data show, for the first time, that the chemical form of Se at nutritional doses can affect the absorptive (apical to basolateral side) efficacy and retention of Se by intestinal cells; but that, these effects are not directly correlated to the potential to support GPx activity.

show abstract

Enteral yeast-selenium supplementation in preterm infants

Cited by 13 publications

References 4 publications

A Selenium-deficient Caco-2 Cell Model for Assessing Differential Incorporation of Chemical or Food Selenium into Glutathione Peroxidase

A Selenium-deficient Caco-2 Cell Model for Assessing Differential Incorporation of Chemical or Food Selenium into Glutathione Peroxidase

Dietary selenium and selenoprotein function

Chemical Form of Selenium Affects Its Uptake, Transport, and Glutathione Peroxidase Activity in the Human Intestinal Caco-2 Cell Model

Contact Info

Product

Resources

About