Entamoeba histolytica up-regulates the Cdc48-like protein, an AAA family member, during the activation of trophozoites with collagen type I and calcium

León-Ávila, Gloria; Hernández, Manuel Hernández; Camacho-Nuez, Minerva; Luna-Arias, Juan Pedro; Salazar, Ma Isabel; Pérez, A; Muñoz, Marı́a de Lourdes

doi:10.1016/j.molbiopara.2005.10.021

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…Another gene more expressed in CSP-R is the Cdc48-like protein (fragment BH18.36). This gene is also more expressed in E. histolytica stimulated by type I collagen and calcium ATPase activity presenting and participating in the processes of endo- and exocytosis [27]. E. histolytica is known for its high content of vesicles containing aggressive molecules such as lysozyme, pore-forming peptides, and proteinases.…”

Section: Discussionmentioning

confidence: 99%

Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues

Fernandes

Freitas

Martins

et al. 2014

The Scientific World Journal

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Entamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. Due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene transcripts between virulent and nonvirulent cultures. In this study we used the differential display to select gene segments related to invasiveness of amoeba. One Brazilian strain of E. histolytica in two conditions, able or not to cause lesions in experimental animals, was used. RNA from this strain, was used to study the differential expression of genes. 29 specific gene fragments differentially expressed in the virulent strain were selected. By real-time PCR, six of these genes had confirmed their differential expression in the virulent culture. These genes may have important roles in triggering invasive amoebiasis and may be related to adaptation of trophozoites to difficulties encountered during colonization of the intestinal epithelium and liver tissue. Future studies with these genes may elucidate its actual role in tissue invasion by E. histolytica generating new pathways for diagnosis and treatment of amoebiasis.

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Section: Discussionmentioning

confidence: 99%

Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues

Fernandes

Freitas

Martins

et al. 2014

The Scientific World Journal

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“…In fact, trophozoites isolated from infected animals present changes in gene expression with respect to trophozoites cultured in the laboratory [ 9 , 10 ]. It has been also shown that trophozoite interaction with human collagen type I and Ca 2+ triggers transcription of virulence related molecules as the amoebapore C, the cysteine proteinase 5 and a cdc48-like protein [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Structural and functional analysis of the Entamoeba histolytica EhrabB gene promoter

Romero-Díaz

Gómez²,

López-Reyes

et al. 2007

BMC Mol Biol

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A putative calcium-ATPase of the secretory pathway family may regulate calcium/manganese levels in the Golgi apparatus of Entamoeba histolytica

et al. 2018

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Calcium regulates many cellular processes in protozoa, including growth, differentiation, programmed cell death, exocytosis, endocytosis, phagocytosis, fusion of the endosomes of distinct stages with phagosomes, fusion of phagosomes with lysosomes, and recycling the membrane. In Entamoeba histolytica, the protozoa responsible for human amoebiasis, calcium ions are essential for signaling pathways that lead to growth and development. In addition, calcium is crucial in the modulation of gene expression in this microorganism. However, there is scant information about the proteins responsible for regulating calcium levels in this parasite. In this work, we characterized a protein of E. histolytica that shows a close phylogenetic relationship with Ca pumps that belong to the family of secretory pathway calcium ATPases (SPCA), which for several organisms are located in the Golgi apparatus. The amoeba protein analyzed herein has several amino acid residues that are characteristic of SPCA members. By an immunofluorescent technique using specific antibodies and immunoelectron microscopy, the protein was detected on the membrane of some cytoplasmic vacuoles. Moreover, this putative calcium-ATPase was located in vacuoles stained with NBD C6-ceramide, a Golgi marker. Overall, the current findings support the hypothesis that the presently analyzed protein corresponds to the SPCA of E. histolytica.

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Entamoeba histolytica up-regulates the Cdc48-like protein, an AAA family member, during the activation of trophozoites with collagen type I and calcium

Cited by 6 publications

References 54 publications

Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues

Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues

Structural and functional analysis of the Entamoeba histolytica EhrabB gene promoter

A putative calcium-ATPase of the secretory pathway family may regulate calcium/manganese levels in the Golgi apparatus of Entamoeba histolytica

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