Entamoeba histolytica thioredoxin reductase: Molecular and functional characterization of its atypical properties

Structural characterization of Giardia duodenalis thioredoxin reductase ( g TrxR) and computational analysis of its interaction with NBDHEX

“…As a final comment, we have shown [6] that, in presence of NADPH, recombinant gTrxR can reduces DTNB with a catalytic efficiency of 3.2x10 3 M -1 s -1 , ten times higher than that reported for the TrxR of the closely related enteric parasite Entamoeba hystolitica ( 2.3x10 2 M -1 s -1 ) [59]. Thus, it M A N U S C R I P T…”

“…The volume of the sites as found by SiteMap is for Site #1 384.07 Å 3 , for Site #2 659 59. Å 3 , for Site #3 230.21 Å 3 , for Site #4 218.14 Å 3 , for Site #5 52.48 Å 3 , and for Site #6 83.01 Å 3 .…”

Functional thioredoxin reductase from pathogenic and free-living Leptospira spp.

“…3). These results indicate that LinTrxR and LbiTrxRs have similar structural properties than other members of L-TrxR family [19,35].…”

“…Fractions containing pure protein were pooled, concentrated and frozen with 20% (v/v) glycerol at -80 °C. LinAhpC, Trx from Prunus persica (PpeTrxh) and Trx from Entamoeba histolytica (EhiTrx8) were purified as previously described [18,19].…”

“…TrxR activity was measured by monitoring the reduction of DTNB at 405 nm [21] in a reaction mixture comprising 100 mM potassium phosphate, pH 7.0, 2 mM EDTA, were prepared according the methodology described by Arias et al [19].…”