Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody

Ortiz‐Ortiz, Librado; Ximénez, Cecilia; Mendoza, Felipe; Michalak, Colette; Melendro, Emma I.; Oliva, A

doi:10.1016/0014-4894(86)90195-5

Cited by 9 publications

(8 citation statements)

References 10 publications

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“…The ELISA method used in this work showed that the monoclonal anti-E. histolytica could detect the presence of amoebic antigen in fecal samples and was sufficiently sensitive to detect 0.1 pg of this antigen or ten or more E. histolytica trophozoites. As stated in Materials and Methods, this monoclonal antibody has been shown to have the necessary specificity to distinguish between E. histolytica and other Entamoeba species (8). This specificity was also demonstrated here by the lack of reactivity with the other parasites, especially Entamoeba coli, with which Entamoeba histolytica is often confused.…”

Section: Discussionsupporting

confidence: 69%

“…That this monoclonal antibody is specific to strains of E. histolyticu has been demonstrated (8). In an ELISA, this monoclonal antibody was shown to react with soluble antigen from E. histolyticu strains HM1 :IMSS, HM3:IMSS, HM38:IMSS, and HK9:NIH but not from E. moshkovskii, E, invadens, or E. histolyticu-related Laredo strain.…”

Section: Preparation Of Fecal Samplesmentioning

confidence: 93%

“…In this work, we report the development of a diagnostic test for intestinal amebiasis by means of an ELISA in which a well-defined monoclonal antibody, specific for a membrane antigen of E. histolytica (8) was used.…”

Section: Introductionmentioning

confidence: 99%

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Diagnosis of entamoeba histolytica in feces by ELISA

Oliva

Hérión

Capin

et al. 1987

Clinical Laboratory Analysis

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A diagnostic test for intestinal amebiasis fnfamoeba coli, with which Entamoeba was developed by using an ELISA that in-hisfolyfica is often confused. In addition, corporated a well-defined monoclonal an-this test detected not only E. hisfolytica tibody specific for a membrane antigen of trophozoites but also cysts. This technique Entamoeba hisfolytica. The ELISA was will be of great value in the rapid and acfound to be sensitive and quite specific for curate diagnosis of E. histolyfica in human f. hisfolyfica, since there were no cross fecal material. reactions with other parasites, especially

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Section: Discussionsupporting

confidence: 69%

Section: Preparation Of Fecal Samplesmentioning

confidence: 93%

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Diagnosis of entamoeba histolytica in feces by ELISA

Oliva

Hérión

Capin

et al. 1987

Clinical Laboratory Analysis

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“…The method is based on an enzyme-linked immunosorbent assay (ELISA) technique in which we use a monoclonal antibody (C29) directed against one of the E. histolytica surface molecules (Ortiz-Ortiz et al 1986). Optimal conditions for amebic adhesion to monolayers of MDCK cells in 96-well culture plates were the following: incubation of 5´10 4 trophozoites/microwell in 200 ll of 2.0 mM PBS (pH 5.7) by coincubation for 15 min at 37°C with gentle shaking (75 rpm).…”

Section: Study Of Amebic Adhesion To Monolayers Of Mdck Cellsmentioning

confidence: 99%

Effect of zinc on Entamoeba histolytica pathogenicity

Robledo

Carrero

Ortiz‐Ortiz

1999

Parasitology Research

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The present study analyzes the effects of zinc on Entamoeba histolytica activity and on its pathogenicity. Metal activity was evaluated in vitro with regard to the parasite's viability, replication, and adhesion to epithelial cells and in vivo with regard to its pathogenicity. The results obtained in vitro show that zinc at 1.0 mM concentration does not affect amebic viability; however, it does decrease amebic replication and adhesion (P < 0.001). In vivo studies performed on a model of experimental liver abscess in the hamster indicate that the intraperitoneal administration of a single dose of zinc at 48 h after the intrahepatic inoculation of amebic trophozoites significantly inhibits (P < 0.001) abscess development. The results indicate that zinc alters the functionality of the ameba in vitro as reflected by a decrease in replication and adhesion and in vivo as manifested by inhibition of amebic pathogenicity.

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“…A well-defined monoclonal antibody, specific for E. histolytica (12), was employed for the ELISA technique. In brief, each fecal sample was homogenized in 0.01 M phosphate buffer containing 0.15 M NaCl (PBS), pH 7.4, which contained sodium dodecyl sulfate (0.05%), and added on wells of polystyrene previously coated with 10 pg of polyclonal anti-E. histolytica (100 pl) in 0.1 M carbonate buffer, pH 9.6.…”

Section: Elsa Techniquementioning

confidence: 99%

Evaluation of the ELISA test for detection of entamoeba histolytica in feces

Merino

Glender

Muro³

et al. 1990

Clinical Laboratory Analysis

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The clinical utility of an ELISA test with monoclonal antibodies to detect antigen of Entamoeba histolytica in feces was evaluated in 150 patients with gastrointestinal symptoms. Each subject was examined by rectosigmoidoscopy with rectal smear and/or a triple stool search for ova-bacteria-parasite (OBP); in addition, one stool sample was collected for the ELISA test. All the tests were independent and double blind. E. histolytica was detected by OBP and/or rectosigmoidoscopy in 66 patients; 61 patients had other parasites; and in 23, no parasites were identified. Of all patients, 116 were positive for the ELISA test. Of these, E. histolytica was identified in 52. In 47, other parasites were identified and in 17, no parasites were found. The ELISA test with a monoclonal antibody against E. histolytica antigen showed higher sensitivity than the standard diagnostic methods: the ability to detect the presence of E. histolytica antigen regardless of the destruction of the parasite or of the error due to misidentification of the parasite resulting from faulty preparation of the samples.

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Entamoeba histolytica: Specific antigen recognized by a monoclonal antibody

Cited by 9 publications

References 10 publications

Diagnosis of entamoeba histolytica in feces by ELISA

Diagnosis of entamoeba histolytica in feces by ELISA

Effect of zinc on Entamoeba histolytica pathogenicity

Evaluation of the ELISA test for detection of entamoeba histolytica in feces

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