Entamoeba histolytica:Isoprenylation of p21rasand p21rapin Vitro

Shen, Pei-Shen; Sanford, Jack C.; Samuelson, John

doi:10.1006/expr.1996.0008

Cited by 10 publications

(7 citation statements)

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“…Protein prenylation is a general pathway in eukaryotic cells and has been described for several protozoan parasites (12,19,26,34,44), including P. falciparum (5). In addition, our group has characterized farnesylated and geranylgeranylated proteins in P. falciparum and showed that prenylation can be inhibited by terpenes (30).…”

mentioning

confidence: 91%

In VitroandIn VivoAntiplasmodial Activities of Risedronate and Its Interference with Protein Prenylation in Plasmodium falciparum

Jordão

Saito

Miguel

et al. 2011

Antimicrob Agents Chemother

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The increasing resistance of malarial parasites to almost all available drugs calls for the identification of new compounds and the detection of novel targets. Here, we establish the antimalarial activities of risedronate, one of the most potent bisphosphonates clinically used to treat bone resorption diseases, against blood stages of Plasmodium falciparum (50% inhibitory concentration [IC 50 ] of 20.3 ؎ 1.0 M). We also suggest a mechanism of action for risedronate against the intraerythrocytic stage of P. falciparum and show that protein prenylation seems to be modulated directly by this drug. Risedronate inhibits the transfer of the farnesyl pyrophosphate group to parasite proteins, an effect not observed for the transfer of geranylgeranyl pyrophosphate. Our in vivo experiments further demonstrate that risedronate leads to an 88.9% inhibition of the rodent parasite Plasmodium berghei in mice on the seventh day of treatment; however, risedronate treatment did not result in a general increase of survival rates.

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confidence: 91%

In VitroandIn VivoAntiplasmodial Activities of Risedronate and Its Interference with Protein Prenylation in Plasmodium falciparum

Jordão

Saito

Miguel

et al. 2011

Antimicrob Agents Chemother

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“…Several of the proteins that undergo these modifications have been identified and many participate in important cell regulatory functions, particularly signal transduction pathways (Zhang & Casey 1996). Protein prenylation is a general phenomenon in eukaryotic cells and has been described for several protozoan parasites (Lujan et al 1995, Field et al 1996, Shen et al 1996, Ibrahim et al 2001, including P. falciparum (Chakrabarti et al 2002).…”

Section: Mep Pathway In P Falciparum -Various Attemptsmentioning

confidence: 99%

Isoprenoid biosynthesis in the erythrocytic stages of Plasmodium falciparum

Jordão

Kimura

Katzin

2011

Mem. Inst. Oswaldo Cruz

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“…Several of the proteins that undergo these modifications have been identified and may participate in important cell regulatory functions, particularly signal transduction pathways (Zhang & Casey 1996). Protein prenylation is a general phenomenon in eukaryotic cells and has also been described for several protozoan parasites (Lujan et al, 1995;Field et al, 1996;Shen et al, 1996;Ibrahim et al, 2001) including P. falciparum (Chakrabarti et al, 2002). In order to investigate the presence of isoprenylated proteins in P. falciparum, the labeled intermediates [1-(n)-3 H]FPP and [1-(n)-3 H]GGPP were used.…”

Section: Posttranslational Modificationmentioning

confidence: 99%