Entactin and laminin gamma1-chain gene expression in the early chick embryo

Zagris, Nikolas; Chung, Albert E.; Stavridis, Vassilis

doi:10.1387/ijdb.041812nz

Cited by 7 publications

(4 citation statements)

References 19 publications

(23 reference statements)

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“…All these basement membrane components remain present on the dermomyotome (Fig. 2B) after the sclerotomal cells de-epithelialise and disperse (Bajanca et al, 2004;Krotoski et al, 1986;Zagris et al, 2000) and fibronectin remains closely associated with this basement membrane (Cachaço et al, 2005;Ostrovsky et al, 1988).…”

Section: Somitogenesis and The Establishment Of The Dermomyotomementioning

confidence: 99%

The extracellular matrix dimension of skeletal muscle development

Þorsteinsdóttir

Deries

Cachaço

et al. 2011

Developmental Biology

135

116

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Cells anchor to substrates by binding to extracellular matrix (ECM). In addition to this anchoring function however, cell-ECM binding is a mechanism for cells to sense their surroundings and to communicate and coordinate behaviour amongst themselves. Several ECM molecules and their receptors play essential roles in muscle development and maintenance. Defects in these proteins are responsible for some of the most severe muscle dystrophies at every stage of life from neonates to adults. However, recent studies have also revealed a role of cell-ECM interactions at much earlier stages of development as skeletal muscle forms. Here we review which ECM molecules are present during the early phases of myogenesis, how myogenic cells interact with the ECM that surrounds them and the potential consequences of those interactions. We conclude that cell-ECM interactions play significant roles during all stages of skeletal muscle development in the embryo and suggest that this "extracellular matrix dimension" should be added to our conceptual network of factors contributing to skeletal myogenesis.

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Section: Somitogenesis and The Establishment Of The Dermomyotomementioning

confidence: 99%

The extracellular matrix dimension of skeletal muscle development

Þorsteinsdóttir

Deries

Cachaço

et al. 2011

Developmental Biology

135

116

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“…Later in development, perlecan immunofluorescence was intense in the neuroepithelium of the diencephalon, myelencephalon and the neural tube in embryos at the 12 somite stage (HH10-11) and was then decreased in the neuroepithelium, but the intensity of the immunofluorescent signal was shifted to the basement membranes of the encephalic vesicles and the neural tube in the embryos by stage HH17 (29 somites). This reflects the expression of perlecan by the neuroepithelium; other basement membrane components expressed by the neuroepithelium are laminin and entactin/nidogen [Zagris et al, 2005]. In the embryos in culture treated with anti-perlecan neutralizing antibodies ( fig.…”

Section: Discussionmentioning

confidence: 97%

Spatial and Temporal Expression of Perlecan in the Early Chick Embryo

Soulintzi

Zagris²

2007

Cells Tissues Organs

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Perlecan is a major heparan sulfate proteoglycan that binds growth factors and interacts with various extracellular matrix proteins and cell surface molecules. The expression and spatiotemporal distribution of perlecan was studied by RT-PCR, immunoprecipitation and immunofluorescence in the chick embryo from stages X (morula) to HH17 (29 somites). Combined RT-PCR and immunohistochemistry demonstrated the expression of perlecan as early as stage X and its presence may be fundamental to the first basement membrane assembly on the epiblast ventral surface at stage XIII (blastula). Perlecan fluorescence was intense in the cells ingressing through the primitive streak and was strong lining the epiblast ventral surface lateral to the streak at stage HH3–4 (gastrula). At stage HH5–6 (neurula), perlecan fluorescence was low in the neuroepithelium and stronger in the apical surface of the neural plate. At stage HH10–11 (12 somites), perlecan fluorescence was intense in the neuroepithelium and was then essentially nondetectable in the neuroepithelium, and the intensity had shifted to the basement membranes of encephalic vesicles by stage HH17. Perlecan immunofluorescence was intense in neural crest cells, strong in pharyngeal arches, intense in thymus and lung rudiments, intense in aortic arches and in dorsal aorta, strong in lens and retina and intense in intraretinal space and in optic stalk, strong in the dorsal mesocardium, myocardium and endocardium, strong in dermomyotome, low in sclerotome in somites, intense in mesonephric duct and tubule rudiments, intense in the lining of the gut luminal surface. Inhibition of the function of perlecan by blocking antibodies showed that perlecan is crucial for maintaining basement membrane integrity which mediates the epithelialization, adhesive separation and maintenance of neuroepithelium in brain, somite epithelialization, and tissue architecture during morphogenesis of the heart tube, dorsal aorta and gut. An intriguing possibility is that perlecan, as a signaling molecule that modulates the activity of growth factors and cytokines, participates in the signaling pathways that guide gastrulation movements and neural crest cell migration, proliferation and survival, cardiac cell proliferation and paraxial mesoderm (somitic) cell proliferation and segmentation.

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“…With regard to EMT, most studies focus on laminin1 (α1β1γ1), which is present in the embryo from the peri-implantation period forward (99). Type 1 EMT during gastrulation and palate fusion is marked by loss of laminin1 (100). Both type 1 and type 2 EMT are associated with downregulation of laminin1 in vitro and disruption and loss of laminin1 in vivo (88,101).…”

Section: Minor Criteriamentioning

confidence: 99%

Biomarkers for epithelial-mesenchymal transitions

Zeisberg¹,

Neilson²

2009

J. Clin. Invest.

1,993

1,895

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Somatic cells that change from one mature phenotype to another exhibit the property of plasticity. It is increasingly clear that epithelial and endothelial cells enjoy some of this plasticity, which is easily demonstrated by studying the process of epithelial-mesenchymal transition (EMT). Published reports from the literature typically rely on ad hoc criteria for determining EMT events; consequently, there is some uncertainty as to whether the same process occurs under different experimental conditions. As we discuss in this Personal Perspective, we believe that context and various changes in plasticity biomarkers can help identify at least three types of EMT and that using a collection of criteria for EMT increases the likelihood that everyone is studying the same phenomenon -namely, the transition of epithelial and endothelial cells to a motile phenotype.

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Entactin and laminin gamma1-chain gene expression in the early chick embryo

Cited by 7 publications

References 19 publications

The extracellular matrix dimension of skeletal muscle development

The extracellular matrix dimension of skeletal muscle development

Spatial and Temporal Expression of Perlecan in the Early Chick Embryo

Biomarkers for epithelial-mesenchymal transitions

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