Ensiling as biological pretreatment of grass (Festulolium Hykor): The effect of composition, dry matter, and inocula on cellulose convertibility

Abstract

. (2013). Ensiling as biological pretreatment of grass (Festulolium Hykor): The effect of composition, dry matter, and inocula on cellulose convertibility. Biomass & Bioenergy, 58, 303-312. DOI: 10.1016/j.biombioe.2013 Our reference: JBB 3333 Dear Author, Please check your proof carefully and mark all corrections at the appropriate place in the proof (e.g., by using on-screen annotation in the PDF file) or compile them in a separate list. Note: if you opt to annotate the file with software other than Adobe Reader then please also highlight the appropriate place in the PDF file. To ensure fast publication of your paper please return your corrections within 48 hours.For correction or revision of any artwork, please consult http://www.elsevier.com/artworkinstructions.Any queries or remarks that have arisen during the processing of your manuscript are listed below and highlighted by flags in the proof. Location in articleQuery / Remark: Click on the Q link to find the query's location in text Please insert your reply or correction at the corresponding line in the proof Q1 As Refs.[3] and [28] were identical, the latter has been removed from the reference list and subsequent references have been renumbered. Q2Please confirm that given names and surnames have been identified correctly.Please check this box or indicate your approval if you have no corrections to make to the PDF file , Thank you for your assistance. HighlightsEnsiling is studied as a pretreatment for cellulolytic conversion of grass.Ensiling is shown to improve enzymatic cellulose convertibility of grass. Low dry matter improved the ensiling process for organic acids production. High levels of lactic acid after ensiling improved enzymatic cellulose conversion. Grass composition prior to ensiling affected ensiling and cellulose convertibility. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 b i o m a s s a n d b i o e n e r g y x x x ( 2 0 1 3 ) 1 production, but the low dry matter in grass at harvest calls for new pretreatment strategies for cellulosic conversion. In this study, ensiling was tested as a biological pretreatment method of the high yielding grass variety Festulolium Hykor. The biomass was harvested in four cuts over a growing season. Three important factors of ensiling: biomass composition, dry matter (DM) at ensiling, and inoculation of lactic acid bacteria, were assessed in relation to subsequent enzymatic cellulose hydrolysis. The organic acid profile after ensiling was dependant on the composition of the grass and the DM, rather than on the inocula.High levels of organic acids, notably lactic acid, produced during ensiling improved enzymatic cellulose convertibility in the grass biomass. Ensiling of less mature grass gave higher convertibility. Low DM at ensiling (<25%) resulted in the highest cellulose convertibilities, which ranged from 32 to 70% of the available cellulose in the four cuts after ensiling. The study confirms...

Biogas production from catch crops: Increased yield by combined harvest of catch crops and straw and preservation by ensiling

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Ensiling and hydrothermal pretreatment of grass: consequences for enzymatic biomass conversion and total monosaccharide yields

“…In our previous study of the same grass species, ensiling directly improved the glucose yield from 7.8 to 11.4 (w/w)%. In the previous study it was concluded that the pretreatment effect was significantly influenced by the DM and biomass composition, which was distinguished by the relative maturity of the grass [18]. The data obtained in the present study supports that the particular grass cut has a significant influence on the effect of ensiling.The enzymatic hydrolysis experiments were performed repeatedly with different sample preparations.…”

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential

“…Most of the lignin concentrations were lower than the ones reported in literature, however a good variation in lignin content between the different substrates was still achieved. The lignin content of the ensilaged maize was very low (0.8 g/100 g) due to the ensilation process, which can be seen as a biological and prolonged acid pretreatment (Ambye-Jensen et al, 2013).…”