Abstract:our recently developed ensilication approach can physically stabilize proteins in silica without use of a pre-formed particle matrix. Stabilisation is done by tailor fitting individual proteins with a silica coat using a modified sol-gel process. Biopharmaceuticals, e.g. liquid-formulated vaccines with adjuvants, frequently have poor thermal stability; heating and/or freezing impairs their potency. As a result, there is an increase in the prevalence of vaccine-preventable diseases in low-income countries even … Show more
“…An earlier study into the mechanism of ensilication showed a diffusion limited (controlled) cluster aggregation (DLCA) type process. 10 This means the more silica added to the reaction, the faster the ensilication will occur because of the abundance of polymeric silica. 33,34 This was observed by FE-SEM (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…6,7 Ultimately, this study aims to further support the future application of ensilication as a novel approach to biopharmaceutical stabilisation. [8][9][10] In order to remain biologically active, the majority of protein-based biopharmaceuticals must be stored and distributed between 2-8 C in what is referred to as the 'cold chain'. Changes in temperature outside this range lead to inactivation due to cold chain failures that can oen be observed in low-income countries.…”
Ensilication is a novel method of protein thermal stabilisation using silica. It uses a modified sol–gel process which tailor fits a protective silica shell around the protein to enable room temperature storage of biopharmaceuticals.
“…An earlier study into the mechanism of ensilication showed a diffusion limited (controlled) cluster aggregation (DLCA) type process. 10 This means the more silica added to the reaction, the faster the ensilication will occur because of the abundance of polymeric silica. 33,34 This was observed by FE-SEM (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…6,7 Ultimately, this study aims to further support the future application of ensilication as a novel approach to biopharmaceutical stabilisation. [8][9][10] In order to remain biologically active, the majority of protein-based biopharmaceuticals must be stored and distributed between 2-8 C in what is referred to as the 'cold chain'. Changes in temperature outside this range lead to inactivation due to cold chain failures that can oen be observed in low-income countries.…”
Ensilication is a novel method of protein thermal stabilisation using silica. It uses a modified sol–gel process which tailor fits a protective silica shell around the protein to enable room temperature storage of biopharmaceuticals.
Time-resolved small-angle X-ray scattering (SAXS) was used to probe the assembly of cellulose nanocrystals (CNC) and montmorillonite (MNT) over a wide concentration range in aqueous levitating droplets. Analysis of the...
“…An ensilicated vaccine retained its immunogenicity after heat treatment or storage at room temperature for three years. 1 The technique uses silica coat to stabilize the tetanus toxoid protein in the diphtheria-tetanus-pertussis vaccine. The vaccine was immunogenic in mice after the vials were shipped within the UK by ordinary post and after heating to 100°C.…”
Section: Ensilicated Vaccines Might Improve the Cold-chain Problemmentioning
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