Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils

Li, Qin Qin; Wang, En Tao; Chang, Yue; Zhang, Yun Zeng; Zhang, Yan Ming; Sui, Xin; Chen, Wen Feng

doi:10.1099/ijs.0.025049-0

Cited by 57 publications

(27 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study indicate that 59 out of 61 isolates from the three agro-ecological regions were fast growers, while only 2 from region II cultivated soils were slow-growers. Based on this observation and the knowledge that soybean are also nodulated by Ensifer fredii and Rhizobium tropici which are fast growers (Li et al, 2011;Kaur et al, 2012), we speculate that the 59 isolates are in fact Ensifer fredii and/or Rhizobium tropici rather than Bradyrhizobium. Without molecular characterization, this cannot be stated with certainty but beckons further characterization of the isolates using DNA extraction and sequencing tools.…”

Section: Glucose-c Utilization Gram Stain Reaction Growth Rate Mucmentioning

confidence: 99%

“…The main soybean-nodulating rhizobia are Bradyrhizobium japonicum, Bradyrhizobium elkanii, Sinorhizobium xinjiangense, Mesorhizobium tianshanense, Ensifer fredii, and Rhizobium tropici (Shiro et al, 2013). Of these, Bradyrhizobium japonicum and Bradyrhizobium elkanii are both slow growing while Ensifer fredii, and Rhizobium tropici are fast growing (Li et al, 2011). decaying root residues improve soil fertility.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and Biochemical Characterization of Soybean Nodulating Rhizobia Indigenous to Zambia

Kapembwa

Mweetwa

Ngulube³

et al. 2016

SAR

View full text Add to dashboard Cite

Soybean [Glycine max (L.) Merrill] is known for nitrogen fixation by rhizobia present in the soil with which it establishes an efficient symbiosis. In Zambia, current rhizobial inoculants used in soybeans production are based on non-indigenous strains; this creates a need to isolate local strains that can be used for the development of local inoculants for soybeans in Zambian soils. This paper reports the isolation and characterization of rhizobial isolates from virgin and cultivated soils of the three agro-ecological regions of Zambia. Rhizobia were isolated using the Trap Method and characterized using selected morphological and biochemical markers. A total of 61 isolates were isolated on Yeast Extract Mannitol (YEM) agar medium. Isolates varied in colony form, color, margin and texture. From the 61 isolates from the three regions, 87 % were circular, 8 % irregular and 5 % punctuate in form with 100 % convex elevation. The isolates had 88% entire, 10% undulate and 2 % lobate colony margins with different colors -56 % cream, 24 % white, 11 % yellow, 5 % transparent and 3 % pink. Transparent colonies were peculiar to Region I and III while pink colonies were peculiar to Region III. All isolates produced mucous, were gram negative and rod shaped, a characteristic of rhizobial cells. None of the isolates could tolerate extremes of pH (4 and 9) in growth medium but grew well at pH 6.8. All isolates utilized glucose as a source of carbon. Based on the Bromothymol Blue (BTB) assay, 59 isolates were fast growing while two isolates from cultivated soils of region II were slow growing. The fast growing 59 isolates showed an acidic reaction changing the medium from green to yellow, while the others showed an alkaline reaction. Based on the results, the 59 fast-growers could be Ensifer fredii or/and Rhizobium tropici rather than Bradyrhizobium. However, further tests to confirm these findings using ketolactose, genetic characterization and inclusion of reference strains, are still needed and are being recommended here.

show abstract

Section: Glucose-c Utilization Gram Stain Reaction Growth Rate Mucmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological and Biochemical Characterization of Soybean Nodulating Rhizobia Indigenous to Zambia

Kapembwa

Mweetwa

Ngulube³

et al. 2016

SAR

View full text Add to dashboard Cite

show abstract

“…While many studies in the past have focussed on plant species of economic importance, including soybean, common bean, cowpea, chickpea and red clover (Delorme et al, 2003;Laranjo et al, 2004;Kuklinsky-Sobral et al, 2005;Duodu et al, 2007;Hung et al, 2007;Laranjo et al, 2008;Ogutcu et al, 2008;Appunu et al, 2009;Chagas et al, 2010;Li et al, 2010;Pule-Meulenberg et al, 2010), native legume species have generally received less attention. In Belgium, legumes are restricted to the Faboideae subfamily and contain 102 plant species in 30 different genera (Lambinon et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

A large diversity of non-rhizobial endophytes found in legume root nodules in Flanders (Belgium)

Meyer

Beuf

Vekeman

et al. 2015

Soil Biology and Biochemistry

117

View full text Add to dashboard Cite

Statistical analysis grouped the investigated plant species into six clusters according to the presence of particular NRE. However, no correlations could be found within these six clusters towards plant tribes or ecoregions the plants had been sampled from. Cluster analysis of the ecoregions according to the presence of NRE, revealed correlations between bacterial genera and those areas. However, groups present in the ecoregions did not correlate with the groups present in the different plant clusters. When combining our previous study on rhizobial diversity recovered from the same sampling campaign (De Meyer et al., 2011) with the current study, 84.1% of the isolates belonged to the traditional rhizobia groups and only 15.9% were NRE. The Loamy ecoregion yielded the lowest number of culturable NRE (8.04%) and the Campine ecoregion the highest number (24.19%). The present study highlights the frequent presence of these NRE in root nodules. The occurrence of certain rhizobia was correlated with the presence of particular NRE, suggesting their presence may not be accidental, however their functions remain unclear at this point.

show abstract

“…Ensifer kummerowiae, Ensifer medicae, Ensifer meliloti, Ensifer mexicanus, Ensifer morelense, Ensifer numidicus, Ensifer psoraleae, Ensifer saheli, Ensifer sesbaniae, Ensifer sojae and Ensifer terangae (Casida Jr, 1982;Li et al, 2011;Lloret et al, 2007;Martens et al, 2008;Merabet et al, 2010;Wang et al, 2013;Yan et al, 2016;Young, 2003). In order to clarify the accurate taxonomic position of this novel Ensifer group, we performed the present study to compare it with the defined Ensifer species.…”

mentioning

confidence: 99%

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Yan

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

A group of Sesbania cannabina rhizobia belonging to four recA genotypes of a novel group was further characterized in comparison with the related Ensifer species. They showed 98.2 to 99.9 % similarities among themselves and 92.9 to 93.3 % similarities with the most related strain Ensifer sojae CCBAU 05684 T in multilocus sequence analysis of recA, atpD and glnII. The genome average nucleotide identity values between representative strain YIC4027 T and the type strains of its closely related species were 81.6 to 88.9 %. Identical symbiotic gene (nodA, nodC and nifH) sequences highly similar with those in other Sesbania-nodulating strains (Rhizobium sp. SIN-1, Neorhizobium huautlense S02 T , Ensifer saheli ORS609 T and Rhizobium sp. IRBG74) were detected. The representative strain YIC4027 T could form effective nodules on its original host Sesbania cannabina, but not on Sophora flavescens, Trifolium repens, Glycine max, Glycyrrhiza uralensis, Phaseolus vulgaris or Medicago sativa. The use of lactulose as sole carbon source, possession of C 13 : 0 2-OH, C 13 : 1 at 12-13, C 15 : 1 iso !9c, C 17 : 0 anteiso and C 18 : 0 iso and absence of C 14 : 0 anteiso, C 15 : 0 anteiso and C 18 : 0 3-OH in fatty acids distinguished the strain YIC4027 T from the type strains of its closely related species. Based on all the analyses mentioned above, we propose a novel species Ensifer alkalisoli sp. nov. and designate YIC4027 T (=HAMBI 3655 T =LMG 29286 T ) as the type strain. The genome size of YIC4027 T is 5.97 Mbp, comprising 5588 predicted genes, and the DNA G+C content is 62.2 mol%.

show abstract

Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils

Cited by 57 publications

References 40 publications

Morphological and Biochemical Characterization of Soybean Nodulating Rhizobia Indigenous to Zambia

Morphological and Biochemical Characterization of Soybean Nodulating Rhizobia Indigenous to Zambia

A large diversity of non-rhizobial endophytes found in legume root nodules in Flanders (Belgium)

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Contact Info

Product

Resources

About