Enrichment of psychrophilic and acidophilic sulfate-reducing bacterial consortia – a solution toward acid mine drainage treatment in cold regions

Dev, Subhabrata; Galey, Miranda; Chun, Chan Lan; Novotny, Chad; Ghosh, Tathagata; Aggarwal, Srijan

doi:10.1039/d1em00256b

Cited by 8 publications

(3 citation statements)

References 79 publications

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“…The bottles were filled with a 10 mM acetic acid buffer adjusted to pH 4.7 with 1 M HCl or 0.5 M NaOH. This pH value was chosen to mimic the effluent of a BSR reactor, which tends to be higher than the BSR feed due to oxidation of organic matter to acetate and carbonate during sulfate reduction (thus, better modeling conditions for subsequent sulfide immobilization). ,, The bottles were filled to the point of no headspace and crimp-capped to prevent the evolution of H 2 S(g) and CO 2 (g). Reactors were stirred at 500 rpm and allowed to equilibrate for 7 days prior to sulfide exposure.…”

Section: Methodsmentioning

confidence: 99%

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)

Bingham

McCormick

Penn

2023

ACS Earth Space Chem.

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Sulfate-rich wastewater poses ecological hazards to freshwater ecosystems, and sulfate is highly regulated in many Minnesota lakes. Biological sulfate reduction results in the reduction of sulfate to sulfide, and this process is used to remediate acid mine drainage. Theoretically, the aqueous sulfide can be immobilized into a solid-phase material and removed from the aqueous system. This study focuses on sulfide immobilization using iron-bearing waste minerals. Specifically, the extent of reaction of siderite (FeCO 3 ), an abundant ferrous mineral in some mining wastes, with sulfide was studied. Mildly acidic batch reactors containing powdered siderite were consecutively injected with a sodium sulfide solution. Solid reaction products were identified and characterized using powder X-ray diffraction, scanning and transmission electron microscopy, and energy-dispersive Xray spectroscopy. Mackinawite (FeS) appeared to be the most abundant product, with greigite (Fe 3 S 4 ) also detected. Results reveal that the immobilization capacity of sulfide by siderite is limited by the concentration of the Fe 2+ (aq) presented in the system immediately before the initial sulfide exposure as the Fe 2+ (aq) levels are not replenished after sulfidation. These results improve our understanding of the sulfidation of siderite and provide insight to improve the viability of using siderite-containing mining waste rock in a sulfate remediation technology.

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Section: Methodsmentioning

confidence: 99%

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)

Bingham

McCormick

Penn

2023

ACS Earth Space Chem.

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“…Microorganisms are able to survive at high temperatures of about 122°C in thermal environments and at low temperatures of −20°C in the perennially frozen atmosphere [1]. Psychotropics and psychrophilic microbes inhabit almost all of the planet's cold regions, including polar and nonpolar [2,3]. Oceans occupy almost 70% of the Earth's surface, making it a primary environment with a temperature of roughly 4°C [4].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a cold‐active, detergent‐stable protease from Serratia sp. TGS1

et al. 2023

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Psychrophiles are cold‐adapted microorganisms living in cold regions and are known to generate cold‐active enzymes such as proteases, lipases, and peptidases. These types of enzymes are a major part of the market of the food and textile sector. This study aimed to isolate and characterize the cold‐active and detergent‐stable, extracellular protease from psychotrophic bacteria Serratia sp. TGS1 (OQ654005). Protease was purified by gel permeation chromatography using Sephadex G‐75. The specific activity of the purified protease was 250 U/mg at 15°C, with a purification fold of 5.68 and a percentage yield of 60%. The cold active protease was stable within a temperature range of 5–30°C and a pH range of 6–10. Ca+2 and Mg+2 enhanced its activity while chelators like ethylenediaminetetraacetic acid inhibited cold active protease, showing it as metalloprotease in nature. The enzyme was sensitive to Cu+2, Zn+2, and Hg+2, and the proteolytic activity decreased upon treatment with heavy metals. The molecular weight of the protease was estimated to be 47 kDa using sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis. Proteins within a specific range of molecular weight possess desirable properties for industrial enzyme use. By working on a specific range, the researchers intended to examine an enzyme to examine its specific characteristics. The purified protease showed high stability to detergents like SDS, Tween 20, Tween 60, and Triton X. The maximum velocity Vmax and Km values were 59.90 mg/min/mL and 1.53 mg/mL, respectively. The obtained protease exhibited an interesting activity at a broad range of pH (6–10) and stability at low temperatures (5–30°C) and detergents. Such enzymatic features of versatile and potent cold‐active enzymes enhance their industrial applications to meet food, dairy, and laundry requirements.

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“…Sulfide is one of the most critical environmental problems in the mining industry [13][14][15]. Sulfur-oxidizing bacteria (SOB) can also increase AMD production resulting in environmental problems [16][17][18]. The control of such disease, once it has developed at a mine, can be difficult and expensive.…”

Section: Introductionmentioning

confidence: 99%

Numerical Evaluation of Two-dimensional Multi-layer Cover System to Regulate Acid Mine Drainage of Tailing Dams

Marandi

Ghiasi

Badv

2023

IJE

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In the mining industry, cover systems for tailings are an effective means of reducing acid mine drainage. An example of this type of system is the multi-layer cover system that is used in arid climates where the annual evaporation rate is greater than the annual rainfall. For users and designers of mine tailings dams, the current research is aimed at identifying the optimal cover system and engineering of the mine waste disposal site, as well as investigating effective geotechnical parameters in controlling the oxygen gas entering the mine waste disposal site. In this research, after careful examination of scientific literature and data collection, it is first validated using numerical modeling, Finite Element Method (FEM) based on the VADOSE/W software, and then modeling is done based on the collected data. One-dimensional modeling has often been used in studies on the evaluation of cover systems, but in this research, twodimensional modeling has been used to analyze the behavior of coating systems. The key to the successful operation of cover systems is maintaining the storage layer at a saturation level of about 85% throughout the year. The two cover systems, "storage and release", "optimized storage and release," are ineffective in maintaining the storage layer at about 85% saturation. However, the capillary barrier cover system has worked successfully and maintained the degree of saturation of the storage layer at about 80%. Due to the use of low-sulfide waste material as an oxygen-consuming layer, the performance of all three cover systems was acceptable. However, it is worth noting that the capillarity barrier cover system was able to immediately cut off the diffusion of oxygen due to the high degree of saturation of the storage layer, while in the other two cover systems, this decrease in diffusion and oxygen concentration was gradual. Therefore, the capillary barrier cover system is suggested as the most optimal system according to the weather conditions and the type of waste materials.

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Enrichment of psychrophilic and acidophilic sulfate-reducing bacterial consortia – a solution toward acid mine drainage treatment in cold regions

Abstract: Failure of sulfate-reducing bacteria (SRB)-mediated treatment of acid mine drainage (AMD) in cold regions due to inhibition of bacteria by acidic pH and low temperature can be overcome by enriching...

Cited by 8 publications

References 79 publications

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)

Isolation and characterization of a cold‐active, detergent‐stable protease from Serratia sp. TGS1

Numerical Evaluation of Two-dimensional Multi-layer Cover System to Regulate Acid Mine Drainage of Tailing Dams

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Enrichment of psychrophilic and acidophilic sulfate-reducing bacterial consortia – a solution toward acid mine drainage treatment in cold regions

Abstract: Failure of sulfate-reducing bacteria (SRB)-mediated treatment of acid mine drainage (AMD) in cold regions due to inhibition of bacteria by acidic pH and low temperature can be overcome by enriching...

Cited by 8 publications

References 79 publications

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO3)

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO3)

Isolation and characterization of a cold‐active, detergent‐stable protease from Serratia sp. TGS1

Numerical Evaluation of Two-dimensional Multi-layer Cover System to Regulate Acid Mine Drainage of Tailing Dams

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Product

Resources

About

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)

Sulfide Immobilization: Investigating the Impact of a Protective Sulfide-Bearing Layer Formed by Siderite (FeCO₃)