Enrichment of normal progenitors in counter‐flow centrifugal elutriation (CCE) fractions of fresh chronic myeloid leukemia leukapheresis products

Dłubek, Dorota; Dybko, Jarosław; Wysoczańska, Barbara; Laba, Anna; Klimczak, Aleksandra; Kryczek, Ilona; Konopka, L; Lange, Andrzej

doi:10.1034/j.1600-0609.2002.01682.x

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…Gradient centrifugation and (immuno)-magnetic activated cell sorting (MACS) are most commonly used [4]. Another technique, continuous counter-flow elutriation, separates cells into multiple fractions [5][6][7][8]. In principle, a constant centrifugal force that separates the cells by density counters a continuously increasing media flow streaming through the sediment dispersing the cells by size.…”

Section: Introductionmentioning

confidence: 99%

Isolation of monocytes from whole blood-derived buffy coats by continuous counter-flow elutriation

2006

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Monocytes (MOs) are the most commonly used precursors for the generation of dendritic cells (DCs) in vitro. Continuous counter-flow elutriation represents a promising tool to isolate MOs from white blood cell (WBC) products. Thirty whole blood-derived, AB0-identical buffy coats (BCs) were pooled using sterile technique (n = 5 experiments). For red blood cell (RBC) and polymorphonuclear cell (PMN) depletion, the BC pools were processed in a Cobe Spectra device (Gambro BCT) using the bone marrow program. Subsequently, continuous counter-flow elutriation in an Elutra device (Gambro BCT) was performed to enrich and purify MOs. BC pool volume averaged 1,260 +/- 14 ml containing 7.7 +/- 1.1 x 10(9) MOs. During 107 +/- 7 min, Cobe Spectra operation, the BC pools were processed for several times, and approximately 9,749 +/- 605 ml volume passed the device. Product volume and MO yield averaged 160 +/- 16 ml, and 4.3 +/- 1.3 x 10(9) cells, respectively. Elutra operation was performed within 59 +/- 0 min and yielded 2.5 +/- 0.9 x 10(9) MOs with a purity of 60 +/- 12%. Compared with the Cobe Spectra product cell count, MO recovery by Elutra averaged 59 +/- 10%. Elutriation of MOs from pooled BCs using Elutra exhibited comparatively low recovery and purity rates. This shortcoming may be due to the nature of the source material. Optimization of the elutriation procedure is necessary to improve MO enrichment from BCs.

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Section: Introductionmentioning

confidence: 99%

Isolation of monocytes from whole blood-derived buffy coats by continuous counter-flow elutriation

2006

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“…For clinical applications, CCE has been used to enrich monocytes from a large volume of peripheral blood mononuclear cells [32]. Recently, there has been interest in CCE as a method to separate lymphocyte subpopulations from monocytes and granulocytes [33], as well as to enrich tumor cell [34] and progenitor cell populations [35]. Primitive progenitors within mouse BM have been isolated using CCE from the earliest elutriated fractions on the basis of size and shown to contribute to lymphohematopoietic reconstitution [36], as well as multilineage engraftment to epithelial tissues in recipient mice [37].…”

Section: Introductionmentioning

confidence: 99%

Identification and Isolation of Small CD44-Negative Mesenchymal Stem/Progenitor Cells From Human Bone Marrow Using Elutriation and Polychromatic Flow Cytometry

Hall

Jiang

Leary

et al. 2013

Stem Cells Translational Medicine

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ABSTRACT؉ cells that lack CD44, an antigen that is highly expressed on cultureexpanded MSCs. In culture, these MSPCs adhere to plastic, rapidly proliferate, and acquire CD44 expression. They form colony forming units-fibroblast and are able to differentiate into osteoblasts, chondrocytes, and adipocytes under defined in vitro conditions. Their acquired expression of CD44 can be partially downregulated by treatment with recombinant human granulocyte-colony stimulating factor, a response not found in BM-MSCs derived from conventional plastic adherence methods. These observations indicate that MSPCs within human BM are rare, small CD45 − CD73 ؉ CD90 ؉ CD105 ؉ cells that lack expression of CD44. These MSPCs give rise to MSCs that have phenotypic and functional properties that are distinct from those of BM-MSCs purified by plastic adherence. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:567-578

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“…For pragmatic outcomes, biosensor-based technologies for cancer clinical testing must incorporate welldefined sample collection procedures and enrichment systems to conversely recover different biomarkers from tumor cells and it becomes necessary to separate few targeted cancer cells from a large volume of normal cells. Various enrichment methods to select targeted cancer cells have been developed which includes density gradient centrifugation [41,42] providing mean tumor cell recovery rates of ∼86% , counter-flow centrifugal elutriation (CCE) [43], immuno magnetic enrichment of cancer cells (more specific) [44,45,46,47,48 49,50] that allows 2300-fold [51] or 8139-fold enrichment. Flow cytometry is a process that separates cancer cells using several biomarkers simultaneously [51,52,53,54].…”

Section: Sample Preparation and Cancer Cell Enrichmentmentioning

confidence: 99%

Biomarkers: A Comprehensive Review and Its Role in Cancer Treatment

Patil¹,

Vyas²,

Singh³

2016

International Journal of Innovative Research in Electronics and

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The paper gives an overview regarding biomarkers their rudimentary characteristics and the abridgment between biomarkers and cancer treatment. The different type of biomarkers and their utility in different environments and conditions gives us cutting edge in terms of cancer treatment. Biomarkers have indeed brought a revolution in the field of medical diagnosis. They have proven to be harbingers of change in the clinical industry. They have a dual functionality and may be used alone or in combination to monitor the health or disease state of an individual. Various clinical assessment techniques have been carried out using biomarkers. The paper gives brief review about biomarkers and also studies various developments that have been done in the recent years.

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Enrichment of normal progenitors in counter‐flow centrifugal elutriation (CCE) fractions of fresh chronic myeloid leukemia leukapheresis products

Abstract: In the present study we have shown that CCE may be used effectively to obtain nLP fractions enriched in normal hematopoietic progenitors.

Cited by 10 publications

References 21 publications

Isolation of monocytes from whole blood-derived buffy coats by continuous counter-flow elutriation

Isolation of monocytes from whole blood-derived buffy coats by continuous counter-flow elutriation

Identification and Isolation of Small CD44-Negative Mesenchymal Stem/Progenitor Cells From Human Bone Marrow Using Elutriation and Polychromatic Flow Cytometry

Biomarkers: A Comprehensive Review and Its Role in Cancer Treatment

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