Enrichment of Methylosinus-dominant consortia from mangroves for polyhydroxybutyrate (PHB) production

Cardoso, Letícia Oliveira Bispo; Karolski, Bruno; Gracioso, Louise Hase; Borrego, Bruna Bacaro; Nascimento, Cláudio Augusto Oller do; Perpétuo, Elen Aquino

doi:10.1016/j.jece.2022.108490

Cited by 7 publications

(7 citation statements)

References 51 publications

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“…The relative abundance of Type II methanotrophs in AS-only cultures remained below 10%, suggesting that Type II methanotrophs require extended adaptation periods for their enrichment. From the perspective of methylotrophs, Methylophilus, a non-methanotrophic methylotroph capable of utilizing methanol, was frequently observed in methanotrophic enrichments in previous studies [33,[45][46][47][48][49]. Methanotrophic enrichments coexisting with methylotrophs and heterotrophs can eliminate toxic metabolites (e.g., methanol and formate) from methanotrophs' methane oxidation.…”

Section: Microbial Community Analysismentioning

confidence: 97%

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Eam,

Ko,

Lee

et al. 2024

Microb Cell Fact

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Background Wastewater treatment plants contribute approximately 6% of anthropogenic methane emissions. Methanotrophs, capable of converting methane into polyhydroxybutyrate (PHB), offer a promising solution for utilizing methane as a carbon source, using activated sludge as a seed culture for PHB production. However, maintaining and enriching PHB-accumulating methanotrophic communities poses challenges. Results This study investigated the potential of Methylosinus trichosporium OB3b to bioaugment PHB-accumulating methanotrophic consortium within activated sludge to enhance PHB production. Waste-activated sludges with varying ratios of M. trichosporium OB3b (1:0, 1:1, 1:4, and 0:1) were cultivated. The results revealed substantial growth and methane consumption in waste-activated sludge with M. trichosporium OB3b-amended cultures, particularly in a 1:1 ratio. Enhanced PHB accumulation, reaching 37.1% in the same ratio culture, indicates the dominance of Type II methanotrophs. Quantification of methanotrophs by digital polymerase chain reaction showed gradual increases in Type II methanotrophs, correlating with increased PHB production. However, while initial bioaugmentation of M. trichosporium OB3b was observed, its presence decreased in subsequent cycles, indicating the dominance of other Type II methanotrophs. Microbial community analysis highlighted the successful enrichment of Type II methanotrophs-dominated cultures due to the addition of M. trichosporium OB3b, outcompeting Type I methanotrophs. Methylocystis and Methylophilus spp. were the most abundant in M. trichosporium OB3b-amended cultures. Conclusions Bioaugmentation strategies, leveraging M. trichosporium OB3b could significantly enhance PHB production and foster the enrichment of PHB-accumulating methanotrophs in activated sludge. These findings contribute to integrating PHB production in wastewater treatment plants, providing a sustainable solution for resource recovery. Graphical abstract

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Section: Microbial Community Analysismentioning

confidence: 97%

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Eam,

Ko,

Lee

et al. 2024

Microb Cell Fact

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“…The PHA family contains more than 150 structures; among them, polyhydroxybutyrate (PHB) is the most commonly known 157 . Polyhydroxybutyrate is very attractive because it presents properties close to those offered by polypropylene and may replace them in different applications 151 . Despite its strong potential, the large‐scale production process of PHB is limited by the costs involved 158 …”

Section: Biotechnological Potential Of Photoautotrophic Microorganism...mentioning

confidence: 99%

“…As Cardoso et al 151 reported, every plastic ever produced still exists today, as it can take one hundred to one thousand years to complete decomposition. 152,153 Furthermore, plastic waste management is shown to be ineffective.…”

Section: Biopolymersmentioning

confidence: 99%

“…157 Polyhydroxybutyrate is very attractive because it presents properties close to those offered by polypropylene and may replace them in different applications. 151 Despite its strong potential, the large-scale production process of PHB is limited by the costs involved. 158 The main cost is associated with the carbon source used to stimulate the accumulation of this biopolymer.…”

Section: Biopolymersmentioning

confidence: 99%

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Photoautotrophic microorganisms from mangroves: a review of the ecological role and bioproducts of commercial interest

Borrego

Melo

Gracioso

et al. 2023

Biofuels Bioprod Bioref

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Photoautotrophic microorganisms, such as microalgae and cyanobacteria, are known to perform an essential role in ecological functions. Besides, they are considered cell factories, producing various bioproducts of commercial interest. In mangroves, these microorganisms are primarily responsible for the ecosystem's high productivity. Moreover, the unique natural characteristics of mangroves, coupled with the intense pressure from anthropic activities that these ecosystems typically experience, make mangrove photoautotrophic microbiota biotechnologically attractive. In this work, the ecological role and biotechnological potential of photoautotrophic mangrove microorganisms worldwide were evaluated, highlighting, their ecosystem services and bioproducts with environmental and commercial appeal, as well as their strategic and technological application through patent analysis.

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“…Despite the attractive sustainability potential, commercial production of polyhydroxyalkanoates is limited by high production costs [13]. The market price of PHAs ranges from US$ 2.4 to 5.5/kg while that of petroleum-based plastics may be as low as US$ 1.2/kg [14].…”

Section: Introductionmentioning

confidence: 99%

Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

Muigano,

Anami,

Onguso

et al. 2024

International Journal of Polymer Science

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible polymers that are produced by microorganisms as storage materials under limited nutrition and excess carbon. These PHAs have been found to be ideal for replacing synthetic plastics for use in packaging and biomedical applications. In this study, an alkaliphilic and moderately halophilic bacterium Halomonas alkalicola Ext was isolated from Lake Simbi Nyaima in western Kenya and investigated for PHA production. Sudan Black B and Nile Red A staining showed that bacterium had distinct ability for accumulation of PHAs. To optimize PHA production, the bacterium was grown in submerged fermentation under varying culture conditions and different sources and concentrations of carbon and nitrogen. With one-factor-at-a-time (OFTA) approach, optimal PHA yields were obtained after 72 hours at a pH of 10.0, temperature of 35°C, and 2.5% (w/v) NaCl. The bacterium yielded the highest biomass, and PHA amounts on 2% galactose and 0.1% ammonium sulfate as sources of carbon and nitrogen, respectively. A record PHA yield of 0.071 g g-1 with a titer of 1.419±0.09 g/L was achieved from 3.397 g/L of biomass, equivalent to 41.8% PHA content. Using response surface methodology, PHA titer was increased by 1.5% to 1.44 g/L, while PHA content was improved 1.1-fold to 45.57%. Polymer analysis revealed that the extracted PHA was a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) (3−HB:3−HV=92:8) with two copolymer subunits of 3-hydroxyvaryrate (3-HB) and 3-hydroxybutyrate (3-HV). Halomonas alkalicola Ext attained efficient galactose conversion into PHBV under high salinity and alkalinity conditions.

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Enrichment of Methylosinus-dominant consortia from mangroves for polyhydroxybutyrate (PHB) production

Cited by 7 publications

References 51 publications

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge

Photoautotrophic microorganisms from mangroves: a review of the ecological role and bioproducts of commercial interest

Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

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