Enrichment of human platelet phospholipids with linoleic acid diminishes thromboxane release

Immediate and long range effects of the uptake of increased amounts of arachidonic acid

“…Approximately 18 g/d of linoleic acid, the parent polyunsaturated fatty acid, are ingested with a common Western diet. In high doses, linoleic acid displays a variety of effects, e.g., lowering of plasma lipids [64] and prevention of cardiovascular disease [2,43,49]. In Western societies, prospective trials showed that a low linoleic acid content in adipose tissue was a strong and independent risk factor for cardiovascular disease [60,61].…”

Arachidonate metabolism in vascular disorders.

“…Recently, a diacylglycerol lipase inhibitor (RHC80267) was described (38 (20:4 A5,8,11,14), and therefore depend on uptake of arachidonate from plasma. Neither platelets (39) nor endothelial cells (40) can convert linoleate to arachidonate. Since arachidonate levels in plasma are low (-1% of free fatty acid), these cells must have an efficient system for uptake and esterification.…”

Polyunsaturated fatty acids, hyperlipidemia, and thrombosis.

“…146 " 148 When the incubation medium was enriched with linoleic acid, the phospholipids in endothelial cells and platelets increased their linoleic acid content from 5% to 25%, but arachidonic acid levels fell by 25% to 40%. This reduction may be due in part to a A6 desaturase deficiency in endothelial cells that prevents conversion of C18:2 to C204 by interfering with the desaturation of C18:2 to C18:3.…”

“…Whether these changes in arachidonic acid content will regularly translate into equivalent changes in thromboxane or prostacyclin release remains to be determined. 148 …”