Enrichment of glycopeptides using environmentally friendly wood materials

Abstract

Glycoproteomics is one of the main routes to study protein post-translational modifications, and enrichment is one of the important steps. Many hydrophilic materials have been used for glycopeptide enrichment, however...

Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G

“…The rapid digestion and MS detection could be used in the future for fast quantification of OPN with the help of an internal standard. Moreover, ascorbic acid-modified TE microchips (TEA microchips) offered a hydrophilic surface in the monolith and were investigated for IgG glycopeptide enrichment, based on the hydrophilic interaction between glycopeptides and the adsorption material [13,46,47]. After optimization with IgG standard samples, TEA microchips were further assessed with human serum sample with antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and sample from healthy humans.…”

Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography

“…Protein glycosylation, one of the major post-translational modifications, plays a crucial role in the development of some chronic or infectious diseases, such as Alzheimer's disease and various autoimmune and inflammatory diseases [ [43] , [44] , [45] ]. Therefore, it is of great interest to monitor variations in protein glycosylation using highly sensitive analytical methods, such as MALDI-TOF MS peptide profiling [ 46 , 47 ]. However, the MS signal of glycopeptides was suppressed in the presence of high-abundance non-glycopeptides.…”

Recent development in hydrophilic interaction liquid chromatography stationary materials for glycopeptide analysis

“…The delignified balsa wood exhibited good hydrophilic diffusivity and glycopeptide enrichment properties. 75 Kapok fiber, another natural fiber derived from the kapok tree, has the ability to specifically and efficiently enrich sialoglycopeptides. Syringyl lignin and guaiacyl lignin of kapok fiber have been shown to facilitate the separation of sialylglycopeptides from complex peptide mixtures.…”